Lipofectamine® RNAiMAX Transfection Reagent.
Here we demonstrate strong mRNA knockdown in several lines using Silencer® Select siRNA in combination with Lipofectamine® RNAiMAX as the transfection agent.
Transfection of GAPDH with Lipofectamine® RNAiMAX into various cell types.
|% Knockdown relative to neg control|
|HASMC (human aortic smooth muscle)||98.1|
|HEKa (human epidermal keratinocytes, adult)||98.7|
|HRE (human renal epithelium)||98.5|
|NTERA-2 (cells-embryonal carcinoma)||98.7|
|HUVEC (human umbilical vein endothelium)||98.3|
|ADSC (adipose-derived stem cells)||97.6|
|BJ (normal human fibroblast)||96.0|
Figure 1. Silencer® Select siRNAs delivered by reverse transfection using Lipofectamine® RNAiMax reagent induce strong GAPDH knockdown in multiple cell types.
Silencer® Select siRNAs for GAPDH were transfected at 30 nM using 0.15–0.3 µL of Lipofectamine® RNAiMAX reagent per well in 96-well plates, following reverse transfection for the various cell types. More than 95% knockdown was obtained for all cell types.