Oncology Research with Laser Capture Microdissection (LCM)

The heterogeneous nature of tumor biopsies can make it difficult to generate useful genomic data from whole tissue samples. Laser capture microdissection (LCM) is a powerful technique that has become instrumental to researchers interested in comparing the molecular profiles of tumor tissue to surrounding non-tumor cells, thereby unlocking hidden molecular signatures in individual patient tumor samples and leading the way for tailored treatment and personalized medicine. The gentle IR laser of the ArcturusXT™ LCM system preserves tissue morphology and genetic content of captured and neighboring cells, providing researchers with an effective tool to easily verify captured material while simultaneously preserving biomolecule integrity.

Sample workflows
LCM to qRT-PCR workflow

Histogene® LCM Frozen Section Staining Kit

ArcturusXT™ LCM System

CapSure® HS LCM Caps

Single Cell-to CT™ qRT-PCR Kit

TaqMan® OpenArray® Human Cancer Panel

QuantStudio® 12K Flex Real-Time PCR System

Histogene® LCM Frozen Section Staining Kit

ArcturusXT™ LCM System

CapSure® HS LCM Caps

Single Cell-to CT™ qRT-PCR Kit

TaqMan® OpenArray® Human Cancer Panel

QuantStudio® 12K Flex Real-Time PCR System

Next-generation sequencing starting with FFPE tissue samples

ArcturusXT™ LCM System

PicoPure® DNA Extraction Kit

ViiA™ 7 Real-Time PCR System

Ion PGM™ System

Ion AmpliSeq™ Cancer Hotspot Panel

Torrent Suite™ Software

ArcturusXT™ LCM System

PicoPure® DNA Extraction Kit

ViiA™ 7 Real-Time PCR System

Ion PGM™ System

Ion AmpliSeq™ Cancer Hotspot Panel

Torrent Suite™ Software

   

Video: Dr. Chip Petricoin

Learn how Dr. Chip Petricoin at the Center for Applied Proteomics and Molecular Medicine at George Mason University discovered, using the ArcturusXT™ LCM System, that pure tumor samples were affected by the unknown and unknowable amounts of stroma and other cells.

Literature

  Application Note: Gene Expression Profiling of a Single Cell Using Laser Capture Microdissection and OpenArray® Technology

  Application Note: An Approach for Analyzing Genetic Heterogeneity in Retrospective Tumor Samples Using Laser Capture Microdissection, Real-Time PCR, and Next-Generation Sequencing

  Combining highly multiplexed PCR with semiconductor-based sequencing for rapid cancer genotyping.
J Mol Diagn. 2013 Mar;15(2):171-6.
Beadling C, Neff TL, Heinrich MC, Rhodes K, Thornton M, Leamon J, Andersen M, Corless CL.

  Deletion hotspots in AMACR promoter CpG island are cis-regulatory elements controlling the gene expression in the colon.
PLoS Genet. 2009 Jan;5(1):e1000334.
Zhang X, Leav I, Revelo MP, Deka R, Medvedovic M, Jiang Z, Ho SM.

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