High quality controls play an integral role in the successful development and performance of high throughput screens. Thermo Fisher Scientific provides a complete collection of validated positive and negative controls to help you optimize delivery conditions, maximize editing efficiency and establish hit selection criteria. These controls can help you build assays with a better signal to noise ratio and give you more confidence in the hits that emerge from your screens.

Please see the tabs below for more information about controls that are available for the LentiArray CRISPR Libraries, TrueGuide Synthetic gRNA and for assay design recommendations.

Type of control Description Recommended use
Negative controls  Negative controls are non-targeting gRNA sequences that don’t recognize any sequence in the human genome. The negative controls are available in multiple package sizes for use in both assay development and as on plate controls when running your screens.
  • Non-targeting gRNA sequences designed to avoid recognizing any sequence in the human genome
  • Available in multiple package sizes for use in both assay development and as on-plate controls when running your screens
Positive controls

Positive controls are validated gRNA sequences that have demonstrated high level editing efficiency; up to 90% editing efficiency or greater in several cell types.  These controls are intended to be used during assay development to determine the conditions that provide maximum editing efficiency in your cell models. 

Individual gRNAs against specific genes are available to serve as biological controls for both assay development and as on plate positive controls when running your screen.  

Individual LentiArray and TrueGuide gRNAs are available.

  • Validated gRNA sequences that have demonstrated high level editing efficiency with up to 90% editing efficiency or greater in several cell types
  • Intended for use during assay development to determine the conditions that provide maximum editing efficiency in your cell models
  • Individual gRNAs against specific genes are available to serve as biological controls for both assay development and as on-plate positive controls when performing your screen
Delivery controls Specific to the LentiArray CRISPR libraries, Thermo Fisher Scientific offers a set of delivery controls. These control lentiviruses are available as either negative or positive controls and also express GFP. The inclusion of the GFP marker provides a visual readout to aid in the rapid optimization of viral delivery conditions.
  • Delivery controls developed specifically for use with the LentiArray CRISPR libraries  
  • Available as either negative or positive controls that also express GFP.  
  • The GFP marker provides a visual readout to aid in the rapid optimization of viral delivery conditions

LentiArray CRISPR Library Controls

Multiple controls are available to support assay development and screening with the LentiArray CRISPR libraries. LentiArray CRISPR Library Controls are delivered as high-titer ready to use lentivirus.

Optimize your assay design with GFP control particles

HT1080 cells infected with the viral particles and control particles expressing GFP provide a visual readout of transduction efficiency and help to determine MOI for the cell line being used in the screen (Figure 1). This illustrates how GFP control particles can be used for optimizing your assay design.

GFP controls particles

Figure 1.  HT1080 cells infected with the viral particles and control particles with GFP. 

User Guides

Type of control Description Recommended use
Negative controls  Negative controls are non-targeting gRNA sequences that don’t recognize any sequence in the human genome. The negative controls are available in multiple package sizes for use in both assay development and as on plate controls when running your screens.
  • Non-targeting gRNA sequences designed to avoid recognizing any sequence in the human genome
  • Available in multiple package sizes for use in both assay development and as on-plate controls when running your screens
Positive controls

Positive controls are validated gRNA sequences that have demonstrated high level editing efficiency; up to 90% editing efficiency or greater in several cell types.  These controls are intended to be used during assay development to determine the conditions that provide maximum editing efficiency in your cell models. 

Individual gRNAs against specific genes are available to serve as biological controls for both assay development and as on plate positive controls when running your screen.  

Individual LentiArray and TrueGuide gRNAs are available.

  • Validated gRNA sequences that have demonstrated high level editing efficiency with up to 90% editing efficiency or greater in several cell types
  • Intended for use during assay development to determine the conditions that provide maximum editing efficiency in your cell models
  • Individual gRNAs against specific genes are available to serve as biological controls for both assay development and as on-plate positive controls when performing your screen
Delivery controls Specific to the LentiArray CRISPR libraries, Thermo Fisher Scientific offers a set of delivery controls. These control lentiviruses are available as either negative or positive controls and also express GFP. The inclusion of the GFP marker provides a visual readout to aid in the rapid optimization of viral delivery conditions.
  • Delivery controls developed specifically for use with the LentiArray CRISPR libraries  
  • Available as either negative or positive controls that also express GFP.  
  • The GFP marker provides a visual readout to aid in the rapid optimization of viral delivery conditions

LentiArray CRISPR Library Controls

Multiple controls are available to support assay development and screening with the LentiArray CRISPR libraries. LentiArray CRISPR Library Controls are delivered as high-titer ready to use lentivirus.

Optimize your assay design with GFP control particles

HT1080 cells infected with the viral particles and control particles expressing GFP provide a visual readout of transduction efficiency and help to determine MOI for the cell line being used in the screen (Figure 1). This illustrates how GFP control particles can be used for optimizing your assay design.

GFP controls particles

Figure 1.  HT1080 cells infected with the viral particles and control particles with GFP. 

User Guides

Resources

  • Learning Center
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  • CRISPR Validated Protocols
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  • Webinar Series
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