The addition of primary amine groups to oligonucleotides are utilized to attach a variety of modifiers (eg. NHS ester fluors) or the attachment to a solid surface. Choose from either 5′-Aminohexyl Linker or TFA-amino linker to add a primary amine to the 5' position of your oligonucleotide sequence. Or for the addition of a phosphate to the 5' position, choose phosphorylating amidite. All three can be found below in the Linkers drop down menu. Spacer phosphoramidites can be utilized to change the oligonucleotide conformation and/or reduce steric hinderance for applications such as aptamers and tagging/labeling assays.
Linker and Spacer Phosphoramidites
- Utilize C3 spacer amidite to incorporate a three carbon spacer or add sequentially to create longer spacer arms.
- Select C18 HEG amidite to reduce possible steric hinderance effects and/or protection from exonucleases.
- An abasic oligo can be formed using the dSpacer amidite.
Click images to enlarge
|dSpacer-CEP||C3-Spacer-CEP||HEG (Spacer 18) phosphoramidite|
|Molecular weight||602.7 g/mol||578.7 g/mol||784.9 g/mol|
|HPLC purity||≥ 95%||≥ 98.0%||≥ 95.0%|
|31P NMR purity||≥ 95%||≥ 98.0%||≥ 98%|
|Material Source||non-animal source (no TSE/BSE risk)|
|Shipping / Storage condition||Dry ice / –20 °C|
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Process and supply chain control
- Processes are monitored from material procurement through final QC and product release under updated ISO9001:2015 standards, providing batch-to-batch reproducibility
- A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
- Purity of each spacer-modified phosphoramidite lot is assessed by HPLC and 31P NMR analyses
- Rigorously controlled manufacturing processes minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide and result in the accumulation of by-products
- Common oligo synthesizer pack sizes are available for immediate delivery
- Custom and bulk packaging are also available. Contact us to learn more.
- Custom manufacturing services are available to assist in the development or scale-up of phosphoramidite compounds
- Specialty phosphoramidites can be manufactured with base or sugar (2′, 3′ or 5′ positions) modifications
Not finding what you are looking for? We also offer DNA phosphoramidites, RNA phosphoramidites, specialty phosphoramidites, dye-labeled phosphoramidites, 2′-modifications, and succinates and CPG support synthesis supports. Contact us to learn more.
Other primers, probes, and nucleotides
For Research Use Only. Not for use in diagnostic procedures.