Biotin-Binding

Capture biotinylated targets

We offer a variety of streptavidin- and NeutrAvidin-coated plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. 

The figure above demonstrates coupling of a biotinylated protein to the covalently bound streptadivin. After a prewash, simply add the biotinylated target molecule in an appropriate buffer. In a short incubation step, the biotinylated molecule will bind to the streptavidin molecule.


Biotin-binding plate types

Nunc streptavidin

  • Nunc Immobilizer streptavidin—The streptavidin protein molecules are covalently bound to the surface via a spacer arm to reduce leaching and enhance precision. The surface is also modified to minimize non-specific binding. As a result, a high S/N ratio is produced, for increased sensitivity. A biotin binding capacity of 20 pmol per well produces excellent analytical results.

Pierce streptavidin

  • Pierce Streptavidin Coated Plates—pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays
  • Pierce Streptavidin Coated High Capacity Plates—designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates
  • Pierce Streptavidin Coated High Sensitivity Plates—biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte

Pierce NeutrAvidin

NeutrAvidin protein is a deglycosylated form of avidin, which has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates off-target binding to the RGD binding domain of adhesion receptors present in a variety of cells.

  • Pierce NeutrAvidin Protein Coated Plates—pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays
  • Pierce NeutrAvidin Coated High Capacity Plates—designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates
  • Pierce NeutrAvidin Coated High Sensitivity Plates—biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte

Related categories

Biotinylation reagents and kits

Biotinylation reagents are available for targeting specific functional groups or residues, including primary amines, sulfhydryls, carboxyls, and carbohydrates. Photoreactive biotin compounds that react nonspecifically upon exposure to ultraviolet (UV) light are also available and expand the scope of the molecules that may be biotinylated. 


FAQs

A selection of FAQs is listed below. For more technical information and FAQs, please visit our Solid Phase Guide ›

When working with the Immobilizer streptavidin plates, instant coupling of biotinylated biomolecules will occur. The Immobilizer streptavidin plate is especially designed and optimized for detection of biotinylated oligonucleotides, peptides, and proteins.

We recommend using transparent polystyrene plates and strips for colorimetric assays, white polystyrene plates for bio and chemiluminescence assays, and black polystyrene plates for fluorescence assays.

Coupling of the target molecule to the streptavidin proceeds very fast. Results show that incubation with gentle agitation for two hours at room temperature ensures that all available active sites are saturated.