Superior reproducible performance with Novex Tris-Glycine Gels

Invitrogen Novex Tris-Glycine Gels are robust gels for a wide range of sample types and molecular weights. They offer sharp straight bands and easier-to-load, larger capacity wells

Novex Tris-Glycine Mini Gel, WedgeWell format

The unique wedge-shaped well in every Invitrogen Novex Tris-Glycine Mini Gel provides higher loading capacity, so you can load up to twice as much protein sample in every well. 

Well size Recommended loading volume Maximum loading volume Maximum protein load
10-well 40 µL 60 µL 0.5 µg/band
12-well 30 µL 45 µL 0.4 µg/band
15-well 20 µL 35 µL 0.25 µg/band
17-well 15 µL 30 µL 0.2 µg/band

Detect proteins even in dilute samples or measure expression of low-abundance proteins. With more room to load your sample, you no longer have to worry about sample spillover and cross-well contamination on your western blots. Gel loading is also easier with no need to use special gel-loading pipette tips.

Increased sample volume capacity of Novex Tris-Glycine Gels
Increased sample volume capacity of Novex Tris-Glycine Gels, WedgeWell format. (A) Increasing volumes (20 μL–60 μL) of a fluorescent protein ladder were loaded in every other lane of an Invitrogen Novex Tris-Glycine 10-well Gel. (B) Increasing volumes (20 μL–60 μL) of the same fluorescent protein ladder were loaded in every other lane of supplier B gel. In gel B, sample spillover is observed as signal in lanes adjacent to the 50-μL and 60-μL load lanes.

Novex Tris-Glycine Gel band quality

Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.

Band quality with tris-glycine gels

Band quality with tris-glycine gels. Protein ladders, purified proteins, and E. coli lysate were loaded on an Invitrogen Novex Tris-Glycine Gel, 4–20% gradient (A) and a supplier 4–20% gradient gel (B). Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A). Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate (10-µL sample volume); lane 4: 6 µg BSA (10-µL sample volume); lane 7: 6 µg hIgG (10-µL sample volume); lane 8: 20 µg E. coli lysate (20-µL sample volume).
 

Novex Tris-Glycine Gels deliver sharp straight bands
Novex Tris-Glycine Gels deliver sharp straight bands. Protein ladders and A431 cell lysate were loaded on a Novex Tris-Glycine Gel, 4–20% gradient and transferred to nitrocellulose using the Invitrogen iBlot 2 Gel Transfer Device. Lane 1: Invitrogen iBright Prestained Protein Ladder; Lane 2: Invitrogen MagicMark XP Western Protein Standard; Lanes 3–7: A431 cell lysate, 15 µg, 5 µg, 1.67 µg, 0.55 µg, 0.19 µg.

With Novex Tris-Glycine Gels, you can achieve greater protein integrity as compared to other commercially available precast gels.

Novex Tris-Glycine Gels offer increased protein integrity
Novex Tris-Glycine Gels offer increased protein integrity. Protein ladder, purified proteins, and E. coli lysate were loaded on a 4–20% gradient Novex Tris-Glycine Gel (A) and a supplier 4–20% gradient gel (B). Gel (B) displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in gel (A). Gel (A) also displays better lysate protein band sharpness and resolution than gel (B). Lanes 1, 10: 5 µL Mark12 Unstained Standard; lane 2: 10 µg E. coli lysate (10-µL sample volume); lane 3: 6 µg catalase (10-µL sample volume); lane 4: 6 µg carbonic anhydrase (10-µL sample volume); lane 5: 6 µg lysozyme (10-µL sample volume); lane 6: 6 µg hIgM (10-µL sample volume); lane 7: 6 µg BSA (10-µL sample volume); lane 8: 6 µg beta-galactosidase (10-µL sample volume); lane 9: 20 µg E. coli lysate (20-µL sample volume).
Migration chart for Tris-Glycine Gels, WedgeWell format
Migration chart for Tris-Glycine Plus Gels

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