Tracking cell division using CellTrace Blue reagent. Human peripheral blood mononuclear cells (PBMCs) were stained with 10 μM Invitrogen CellTrace Blue reagent. The blue peaks represent successive generations of cells stimulated with Gibco Dynabeads Human T-Expander CD3/CD28 and cultured for 5 days. The light blue peak represents cells that were cultured with no stimulus. Data were collected on a BD LSR II Flow Cytometer with 355 nm excitation and a 450/50 nm bandpass emission filter.
Our newest cell and molecular biology products and technologies
On this page:
- Using CFSE? Have a UV laser? Try our UV-excitable CellTrace Blue cell proliferation reagent
- Super Bright antibody conjugates: Superb alternatives to Brilliant Violet conjugates for flow cytometry
- No-Weigh packaging now available for protein modification reagents
- More Alexa Fluor dye–conjugated organelle-specific antibodies
- Now available: ProLong Glass Antifade Mountant
- Pierce Rapid Gold BCA Protein Assay provides highly accurate results in 5 minutes
Using CFSE? Have a UV laser? Try our UV-excitable CellTrace Blue cell proliferation reagent
The Invitrogen CellTrace Blue Cell Proliferation Kit is an alternative to CFSE for tracing multiple generations by dye dilution using flow cytometry. The CellTrace Blue cell proliferation reagent can be used in the same applications as CFSE and other CellTrace reagents; however, it was specifically designed for excitation by a UV laser (355 nm or 375 nm). The CellTrace Blue reagent can easily be multiplexed with reagents and antibodies that are excited by other commonly used lasers. With the development of the CellTrace Blue dye, we now offer a full portfolio of CellTrace cell proliferation reagents, including the widely used Invitrogen™ CellTrace™ Violet reagent; see the Journal Club article "An evaluation of spectrally distinct succinimidyl ester dyes for tracking cell proliferation by flow and image cytometry." From UV to red laser excitation, we have a CellTrace reagent for your existing flow panels.
See the CellTrace selection guide
Super Bright antibody conjugates: Superb alternatives to Brilliant Violet conjugates for flow cytometry
Invitrogen eBioscience Super Bright antibody conjugates are now available in an even greater variety of markers, clones, and formats. With over 400 products covering more than 100 different clones (including both human and mouse antigens), you have even more options for your violet laser. Discriminate dim cell populations more easily using Super Bright 436, Super Bright 600, Super Bright 645, or Super Bright 702 antibody conjugates for flow cytometry. As compared with Brilliant Violet conjugates, the Super Bright conjugates display similar or better brightness and some have been shown to require less compensation. Discover your options and find a Super Bright antibody conjugate that fits your flow cytometry panel.
Better resolution achieved with Super Bright antibody conjugates vs. APC antibody conjugates. Human peripheral blood mononuclear cells (PBMCs) were stained with PE anti–human CD20 antibody and anti–human CD11c antibody conjugated either to Super Bright 702 dye (left) or to APC (right). CD11c+ cells stained with Super Bright antibodies demonstrate better resolution than those stained with APC antibodies.
No-Weigh packaging now available for protein modification reagents
Thermo Scientific No-Weigh products are specialty protein modification reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents contamination and loss of reagent reactivity over time by eliminating the repetitive opening and closing of the vial. It also eliminates the hassle of weighing small amounts of reagent; a readyto- use solution can be made quickly and conveniently. The No-Weigh packaging format is available in convenient fill sizes using PCR strip tubes or easy-to-handle screw cap vials for popular protein reagents, including reducing agents, crosslinkers, and PEG- and biotin-labeling products.
Find out more about our No-Weigh products
No-Weigh packaging. Thermo Scientific No-Weigh packaging is now available for widely used protein modification reagents such as reducing agents, crosslinkers, and labeling products. An example of the pre-aliquoted screw cap vials is shown here.
More Alexa Fluor dye–conjugated organelle-specific antibodies
Thermo Fisher Scientific has launched additional organelle-specific fluorescent antibodies for use in colocalization and other immunofluorescent experiments. We now offer over 30 different organelle-specific antibodies preconjugated to highly fluorescent Invitrogen™ Alexa Fluor™ dyes. These organelle-specific antibodies are high-affinity monoclonal and polyclonal antibodies that recognize cellular targets normally restricted to specific organelles, including mitochondria, lysosomes, endoplasmic reticulum, nucleus, nucleolus, and more. No secondary detection reagents are required so multiplex experiments are greatly simplified. See all of the organelle-specific antibodies at thermofisher.com/organelleabs.
See all of the organelle-specific antibodies
Multiplexed staining of cell junctions, mitochondria, and nucleoli. Fixed, permeabilized, and blocked Caco-2 cells were labeled with Invitrogen Alexa Fluor 647 anti–ZO-1 (red), Alexa Fluor 488 anti–ATP synthase β (orange), Alexa Fluor 488 anti-nucleophosmin (green), and Hoechst 33342 (blue). Cells were imaged on an Invitrogen EVOS FL Auto Imaging System at 60x magnification.
Now available: ProLong Glass Antifade Mountant
The Invitrogen ProLong Glass Hard-Set Antifade Mountant is a readyto- use mountant with a high refractive index (1.52 after curing) that results in up to 75% more axial resolution and superior lateral resolution when compared with low refractive index mountants. This mountant delivers excellent signal-to-background ratios and photobleaching protection for organic fluorophores and fluorescent proteins, making it ideal for producing sharp, bright, high-quality images of any cell or tissue sample up to 100 μm in thickness with almost any fluorescence microscope.
Find out more about our antifade mountants
Photobleaching protection by ProLong Glass mountant. HeLa cells were labeled with anti-tubulin antibody, detected with fluorescein (FITC) secondary antibody, and mounted in (A) Invitrogen ProLong Glass Hard-Set Antifade Mountant or (B) 50% glycerol in PBS. The samples were then illuminated for 60 sec using a 100 W Hg-arc lamp and imaged using a 20x air objective and a 12-bit monochrome camera.
Pierce Rapid Gold BCA Protein Assay provides highly accurate results in 5 minutes
The Thermo Scientific Pierce Rapid Gold BCA Protein Assay Kit is a high-precision, two-component, detergent-compatible assay for measuring total protein concentration with copper-chelating technology (using absorbance at 480 nm). This assay provides the same accuracy and detergent compatibility as the traditional BCA assay but in only 5 minutes and with a room-temperature incubation that eliminates the need to expose samples to elevated temperatures. The Pierce Rapid Gold BCA assay can be adapted for use with microplate readers and spectrophotometers.
Find out more about our Pierce Rapid Gold BCA Protein Assay Kit
The Pierce Rapid Gold BCA Protein Assay provides the accuracy of the BCA assay with the speed of the Bradford assay. Standard curves for the Thermo Scientific Pierce Rapid Gold BCA Protein Assay, Pierce BCA Protein Assay, and Bio-Rad Bradford Protein Assay were produced using purified BSA in 0.9% saline. All assays were conducted according to the manufacturer’s protocol in a microplate format.
For Research Use Only. Not for use in diagnostic procedures.