ReadyProbes® Cell Viability Imaging Kit (Blue/Green) Protocol
Two-color nuclear staining assay for cell viability
This kit can be used to quickly and easily determine the viability of cells. NucBlue® Live reagent stains the nuclei of all cells, while NucGreen® Dead reagent stains only the nuclei of dead cells.
This protocol can be used for:
- Identifying live and dead cells using a fluorescence microscope
This protocol should not be used for:
- Flow cytometry
|1. Culture cells in appropriate medium and vessel for microscopy
|2. Add 2 drops each NucBlue® Live and NucGreen® Dead reagents per milliliter of medium to label cells
|3. Incubate 5–30 minutes|
|4. Image cells|
Spectral information and storage
|NucBlue® Live||NucGreen® Dead|
|Excitation/Emission||360/460 nm||504/523 nm|
|Standard filter set||DAPI||FITC/GFP|
|EVOS® Light Cube||DAPI||GFP|
|Storage conditions||Room temperature||Room temperature|
- In some cases, more or fewer drops may be needed to achieve optimal staining intensity
- Image quality may be improved by replacing media with Live Cell Imaging Solution (Cat. No. A14291DJ)
Jurkat cells stained with ReadyProbes® (Blue/Green) Cell Viability Imaging Kit.
For Research Use Only. Not for use in diagnostic procedures.