QuantStudio™ 3 and QuantStudio™ 5 Real-Time PCR Systems Support—Troubleshooting
Having difficulties with your experiment?
We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios.
View the relevant questions below:
If you are using a master mix that has ROX™ dye provided in a separate tube, use a low ROX™ dye concentration (50 nM, final well volume).
No. For multi-plate analysis of gene expression data, please use the Applied Biosystems™ qPCR Analysis Module (RQ application) with your Thermo Fisher Cloud account.
No. You can use the QuantStudio™ Design and Analysis Desktop Software, or for multi-plate analysis of genotyping data, please use the Applied Biosystems™ qPCR Analysis Module (GT application) with your Thermo Fisher Cloud account.
Check if the Cloud Monitor is on. Go to Settings --> Maintenance and Service --> Monitoring. Turn CloudSuite Monitor ‘ON’. This needs to be set to ‘ON’, otherwise the instrument will not look out to connect to your account even if you are logged in.
For Research Use Only. Not for use in diagnostic procedures.