In this webinar, sample preparation, LC and MS approaches to address in vivo biotherapeutics will be presented, as well as tissue and plasma analysis methods for biotherapeutics.
LC/MS-related techniques represent promising alternatives to LBA for quantitative investigation of biotherapeutics and biomarkers in pharmaceutical and clinical applications. However, it often remains challenging to achieve sufficient sensitivity, throughput and robustness. Recently developed strategies will be introduced to address these needs, including technical advances in sample preparation, antibody-free enrichment, a trapping micro-LC/MS and new mass spectrometric approaches, followed by several applications. New methods for discovery and quantification of in vivo complexes and fragments of biotherapeutics formed in plasma and tissues will also be detailed.