Reliability you can set your watch to

Move your research forward by integrating a comprehensive multi-component automated workcell by Invitrogen Flow Cytometry.

Workcell components


Maximize operating capacity with a multicomponent automated workcell

Move your research forward by integrating a comprehensive multicomponent automated workcell by Invitrogen Flow Cytometry. Explore the suite of instruments that maximize operating capacity, mitigate human operator error and enable rich, reproducible data.


Flow cytometer

Figure 1. The Attune NxT Flow Cytometer is the instrument paired with the Orbitor RS Microplate Mover for the Invitrogen flow cytometry robotics solution.

The flow cytometer for the robotics workstation is the Invitrogen Attune NxT Flow Cytometer (Figure 1). The Attune NxT Flow Cytometer is a high-performance instrument, engineered to be capable of meeting the rigid demands of a high-throughput environment. Reliability is central to any automation operation, and every component of the Attune NxT Flow Cytometer—from technology and fluidics to optics and software—was developed with features to help address these challenges and help meet the high demands of automated workflows.

Some of the common challenges facing researchers doing high-throughput flow cytometry include:
  • Clogging
  • Time commitment
  • Data inaccuracy

Fluidics

Complete trust of an instrument’s reliability is earned. Robotic capability is inconsequential if paired with an instrument prone to clogging and other malfunctions. The Attune Nxt Flow Cytometer operates with a non-peristaltic, continuous flow pump.

Advantages include:
  • Precise counts–uncompromised volumetric cell counts for all samples in a known volume (gated or total events)
  • Stable flow–low fluid pulsation offers less variation in a particle’s velocity within the flow stream and increased certainty of where the particle will be after traversing a distance within the flow cell
  • Engineering to actively resist clogging–syringe-driven system pushes the sample with a force of up to 75 psi and is drastically less susceptible to clogs caused by backpressure. 

Learn more about the Attune NxT Flow Cytometer

Technology

The Attune flow cytometer uses traditional hydrodynamic focusing for low sample rates 12.5–25 μL/min and acoustic-assisted hydrodynamic focusing (Figure 2) for high sample rates (up to 1,000 μL/min).

Advantages include:
  • Uniform laser illumination–precisely aligned particle stream is maintained regardless of the sample input rate
  • High speed with low coincidence rate–acquire data at up to 35,000 events/second with 34 parameters, based on a 10% coincidence rate per Poisson statistics; maximum electronic speed is 65,000 events per second with all parameters

Figure 2. In this video, Sr. Lead Engineer, Mike Ward describes the thinking behind acoustic focusing and the ten-fold benefits of this capability. 

Learn more about traditional hydrodynamic focusing vs. acoustic-assisted hydrodynamic focusing

Optics

The Attune NxT flow cytometer’s optical design with defined, fixed alignment of up to four (Figure 3) spatially separated lasers offers consistency in data over time, superior performance, and first-class reliability.

Other advantages include:
  • Interchangeable filters–minimized reagent crosstalk and maximized signal
  • Laser simmer mode–ready at all times, but only actively ‘on’ when acquiring samples; end result: 10x increase in laser lifetime
  • Stable alignment–pre-aligned and welded fiber to laser beam–shaping optics (BSO) interface

Table 1. Attune NxT Flow Cytometer configuration options

Lasers Laser configuration
(Cat. No.)
Lasers included, No. of detection channels Total detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
(A24864)
Available as upgrade†
4
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
(A28995)
Available as upgrade†
3
 
4
Available as upgrade† 9
Blue/yellow
(A24861)
Available as upgrade†
3
4
  Available as upgrade† 9
Blue/violet
(A24862)
4
4
Available as upgrade† Available as upgrade† Available as upgrade† 10
Blue/red
(A24863)
Available as upgrade†
4
Available as upgrade† Available as upgrade†
3
9
3 Blue/green/red
(A28997)
Available as upgrade†
3
 
4
3
12
Blue/green/violet
(A28999)
4
3
 
4
Available as upgrade† 13
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/violet/yellow (A24859)
4
3
4
 
Available as upgrade† 13
Blue/red/violet
(A24860)
4
4
Available as upgrade† Available as upgrade†
3
13
4 Blue/red/violet/green
(A29001)
4
3
 
4
3
16
Blue/red/yellow/violet
(A24858)
4
3
4
 
3
16
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser UpgradeRed Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

Fluidics

Complete trust of an instrument’s reliability is earned. Robotic capability is inconsequential if paired with an instrument prone to clogging and other malfunctions. The Attune Nxt Flow Cytometer operates with a non-peristaltic, continuous flow pump.

Advantages include:
  • Precise counts–uncompromised volumetric cell counts for all samples in a known volume (gated or total events)
  • Stable flow–low fluid pulsation offers less variation in a particle’s velocity within the flow stream and increased certainty of where the particle will be after traversing a distance within the flow cell
  • Engineering to actively resist clogging–syringe-driven system pushes the sample with a force of up to 75 psi and is drastically less susceptible to clogs caused by backpressure. 

Learn more about the Attune NxT Flow Cytometer

Technology

The Attune flow cytometer uses traditional hydrodynamic focusing for low sample rates 12.5–25 μL/min and acoustic-assisted hydrodynamic focusing (Figure 2) for high sample rates (up to 1,000 μL/min).

Advantages include:
  • Uniform laser illumination–precisely aligned particle stream is maintained regardless of the sample input rate
  • High speed with low coincidence rate–acquire data at up to 35,000 events/second with 34 parameters, based on a 10% coincidence rate per Poisson statistics; maximum electronic speed is 65,000 events per second with all parameters

Figure 2. In this video, Sr. Lead Engineer, Mike Ward describes the thinking behind acoustic focusing and the ten-fold benefits of this capability. 

Learn more about traditional hydrodynamic focusing vs. acoustic-assisted hydrodynamic focusing

Optics

The Attune NxT flow cytometer’s optical design with defined, fixed alignment of up to four (Figure 3) spatially separated lasers offers consistency in data over time, superior performance, and first-class reliability.

Other advantages include:
  • Interchangeable filters–minimized reagent crosstalk and maximized signal
  • Laser simmer mode–ready at all times, but only actively ‘on’ when acquiring samples; end result: 10x increase in laser lifetime
  • Stable alignment–pre-aligned and welded fiber to laser beam–shaping optics (BSO) interface

Table 1. Attune NxT Flow Cytometer configuration options

Lasers Laser configuration
(Cat. No.)
Lasers included, No. of detection channels Total detection channels*
Violet, 405 nm Blue, 488 nm Yellow, 561 nm Green,
532 nm
Red, 637 nm
1 Blue
(A24864)
Available as upgrade†
4
Available as upgrade† Available as upgrade† Available as upgrade† 6
2 Blue/green
(A28995)
Available as upgrade†
3
 
4
Available as upgrade† 9
Blue/yellow
(A24861)
Available as upgrade†
3
4
  Available as upgrade† 9
Blue/violet
(A24862)
4
4
Available as upgrade† Available as upgrade† Available as upgrade† 10
Blue/red
(A24863)
Available as upgrade†
4
Available as upgrade† Available as upgrade†
3
9
3 Blue/green/red
(A28997)
Available as upgrade†
3
 
4
3
12
Blue/green/violet
(A28999)
4
3
 
4
Available as upgrade† 13
Blue/red/yellow (A28993) Available as upgrade† 3 4   3 12
Blue/violet/yellow (A24859)
4
3
4
 
Available as upgrade† 13
Blue/red/violet
(A24860)
4
4
Available as upgrade† Available as upgrade†
3
13
4 Blue/red/violet/green
(A29001)
4
3
 
4
3
16
Blue/red/yellow/violet
(A24858)
4
3
4
 
3
16
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel.
† Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser UpgradeRed Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options.

Plate sampler

The Invitrogen Attune Autosampler is a high-throughput plate sampler option for the Attune NxT flow cytometer that interfaces with the Orbitor RS robotic arm. This instrument adds microplate capacity to the Attune NxT flow cytometer and by doing so, increases walkaway autonomy, improves experimental workflow, and systematizes the data gathering process.

  • Broad compatibility—compatible with many different plate formats, including 96-well, 384-well, and deep-well plates
  • Intelligent probe—intelligent probe design minimizes clogging and carryover (0.5%) and helps prevent damage to the instrument
  • Auto cleaning—performs automated cleaning when the instrument is shutting down
  • Consistent data—designed to provide minimal variation and reduced probability for sample-switching errors
  • Mixing by aspiration—mixing sample by aspiration instead of shaking helps ensure homogeneity of the sample and cell viability maintenance
  • Visualization software—heat map view allows rapid screening and confirmation of samples while the instrument is acquiring data

Learn more about the Attune NxT Autosampler


Robotic Arm

The Orbitor RS Microplate Mover is a high-speed robotic mover designed for integrated lab automation, offering automatic retrieval of a plurality of microplates in association with a variety of applications, including flow cytometry. Linking one or multiple Attune NxT flow cytometers with one or more Orbitor RS movers will help keep your research moving forward and allow you to scale up to the volume of samples needed for statistical significance and rich data from which to validate research and discovery.

Labs optimized with robotic handling will benefit from the performance, software design, engineering, safety of the Orbitor RS Microplate Mover.

Performance of the Orbitor RS Microplate Mover

The Orbitor RS Microplate Mover is the centerpiece of automation operations.  Incorporating the Orbitor RS into the automation workflow helps increase efficiency and flexibility. The Orbitor RS keeps pace with the Attune NxT Flow Cytometer, an instrument already 10x faster than conventional flow cytometers. The time from storage to instrument is 4 seconds.

Working environment

Especially beneficial in shared lab environments, the Orbitor RS Microplate Mover is one of the quietest movers in its class.

Continuous runtime

Continuously run up to 19.5 hours unattended without fluid resupply. External fluids can be refilled without interrupting operation for extended runtime.

Unattended Mode

The Orbitor RS helps increase operator autonomy with Unattended Mode, which manages the system’s operation during extended unattended operation. This mode has advance error set-up with user-defined escalation. Events can be considered by system error beacon: “ignore” “warning” or “error” (Figure 3). Set email and text notifications to introduce autonomy in your routine and peace of mind during overnight runs.

Figure 3. System error beacon for advanced error notification with “ignore” “warning” or “error” event distinctions.

Straightforward, feature-rich software of the Orbitor RS Microplate Mover

The robotic automation system is managed centrally through Thermo Scientific Momentum Laboratory Automation Workflow Software (Figure 4). Define, execute, and monitor complex processes by the Orbitor RS and Attune NxT flow cytometer in a straightforward, visual environment with many user-defined and custom features.

Home screen

Figure 4. Home screen user interface of the Thermo Scientific Momentum Laboratory Automation Workflow Software.

The Attune NxT software is designed for compatibility with Momentum software (Figure 5).  Automation buttons on the ribbon in Attune NxT software control automation settings. When automation is enabled, Momentum software connects the Orbitor RS to the Attune NxT Flow Cytometer.

Home screen

Figure 5. (A) Home screen of the user-interface of the Attune NxT software. (B) Automation icons on the ribbon of the Attune NxT software.

Safety of the Orbitor RS Microplate Mover

Collision detection and recovery

The Orbitor RS robot was designed with active and passive safety technology features:
  • No jabbing or protruding fingers—an overhead grip features a rubber bumper
  • Self-homing mover–the mover will home itself and open up communication with instrument upon system start-up
  • Advanced error handling—provides recovery options
  • Rapid response—the instrument responds with split-second reaction time upon minimal contact
  • Manual override mechanism—emergency stop button (Figure 6) manually halts operation; user-initiated stops and last known location logged in the system

Figure 6. Emergency stop device hardwired into the Orbitor RS to enable manual operation override. Standard electronic override stopping button ceases all robotic motion is split-second when pressed. 

Engineering of the Orbitor RS Microplate Mover

Barcode reading

For sample traceability, plate data storage, and barcode transmission, the Orbitor RS mover is designed to synchronize barcodes. The Orbitor RS mover has an on-board bar code reader in the grip (Figure 7).

Grip
  • Grip force settings based on plate type and weight with sample
  • Grip detection
  • Integrated plate sensor
  • Bi-directional wrist for portrait or landscape clutching
Diverse plate compatibility
  • A pre-populated list of plate types
  • Additional plate types may be added for a variety of applications
  • Lidded plates to and from storage, and un-lid and re-lid plates
Hotels and stacks
  • Storage platforms configured with an arrangement of plates and hotels (Figure 8)
  • Random-access microplate hotels
  • Sequential-access storage stacks
  • Landscape and portrait plate orientation

Figure 7. Plate gripper with integrated barcode reader. 

Figure 8. Two hotels and two stacks positioned to store and receive well plates. Extended platforms are available for expanded plate capacity.

Performance of the Orbitor RS Microplate Mover

The Orbitor RS Microplate Mover is the centerpiece of automation operations.  Incorporating the Orbitor RS into the automation workflow helps increase efficiency and flexibility. The Orbitor RS keeps pace with the Attune NxT Flow Cytometer, an instrument already 10x faster than conventional flow cytometers. The time from storage to instrument is 4 seconds.

Working environment

Especially beneficial in shared lab environments, the Orbitor RS Microplate Mover is one of the quietest movers in its class.

Continuous runtime

Continuously run up to 19.5 hours unattended without fluid resupply. External fluids can be refilled without interrupting operation for extended runtime.

Unattended Mode

The Orbitor RS helps increase operator autonomy with Unattended Mode, which manages the system’s operation during extended unattended operation. This mode has advance error set-up with user-defined escalation. Events can be considered by system error beacon: “ignore” “warning” or “error” (Figure 3). Set email and text notifications to introduce autonomy in your routine and peace of mind during overnight runs.

Figure 3. System error beacon for advanced error notification with “ignore” “warning” or “error” event distinctions.

Straightforward, feature-rich software of the Orbitor RS Microplate Mover

The robotic automation system is managed centrally through Thermo Scientific Momentum Laboratory Automation Workflow Software (Figure 4). Define, execute, and monitor complex processes by the Orbitor RS and Attune NxT flow cytometer in a straightforward, visual environment with many user-defined and custom features.

Home screen

Figure 4. Home screen user interface of the Thermo Scientific Momentum Laboratory Automation Workflow Software.

The Attune NxT software is designed for compatibility with Momentum software (Figure 5).  Automation buttons on the ribbon in Attune NxT software control automation settings. When automation is enabled, Momentum software connects the Orbitor RS to the Attune NxT Flow Cytometer.

Home screen

Figure 5. (A) Home screen of the user-interface of the Attune NxT software. (B) Automation icons on the ribbon of the Attune NxT software.

Safety of the Orbitor RS Microplate Mover

Collision detection and recovery

The Orbitor RS robot was designed with active and passive safety technology features:
  • No jabbing or protruding fingers—an overhead grip features a rubber bumper
  • Self-homing mover–the mover will home itself and open up communication with instrument upon system start-up
  • Advanced error handling—provides recovery options
  • Rapid response—the instrument responds with split-second reaction time upon minimal contact
  • Manual override mechanism—emergency stop button (Figure 6) manually halts operation; user-initiated stops and last known location logged in the system

Figure 6. Emergency stop device hardwired into the Orbitor RS to enable manual operation override. Standard electronic override stopping button ceases all robotic motion is split-second when pressed. 

Engineering of the Orbitor RS Microplate Mover

Barcode reading

For sample traceability, plate data storage, and barcode transmission, the Orbitor RS mover is designed to synchronize barcodes. The Orbitor RS mover has an on-board bar code reader in the grip (Figure 7).

Grip
  • Grip force settings based on plate type and weight with sample
  • Grip detection
  • Integrated plate sensor
  • Bi-directional wrist for portrait or landscape clutching
Diverse plate compatibility
  • A pre-populated list of plate types
  • Additional plate types may be added for a variety of applications
  • Lidded plates to and from storage, and un-lid and re-lid plates
Hotels and stacks
  • Storage platforms configured with an arrangement of plates and hotels (Figure 8)
  • Random-access microplate hotels
  • Sequential-access storage stacks
  • Landscape and portrait plate orientation

Figure 7. Plate gripper with integrated barcode reader. 

Figure 8. Two hotels and two stacks positioned to store and receive well plates. Extended platforms are available for expanded plate capacity.

Temperature control

Integrating stable temperature storage into a robotic automation system is possible with an optional automation-friendly temperature-controlled storage device (Figure 9). The Thermo Scientific SmartStor benchtop microplate storage device operates within temperatures ranging from 4 to 40°C and offers a plate capacity of 20 standard microplates or 9 deep-well blocks, and self-scanning internal inventory.

Figure 9. The Attune NxT Flow Cytometer operating with the Thermo Scientific Orbitor RS Microplate Mover at the 2017 Society for Laboratory Automation and Screening conference. Plates in configuration are housed at a defined, maintained temperature inside the Thermo Scientific SmartStor microplate storage device.

Expanded fluidics

Figure 10. The Attune NxT External Fluid Supply increases fluid capacity.

 

Configuring a workflow that includes the Invitrogen Attune NxT External Fluid Supply (EFS) reduces the frequency of the fluid management tasks of replenishing and emptying and increases the period of continual operation.

The EFS enables the Attune NxT flow cytometer to run for up to 19 hours continuously, which is supported effortlessly by the continuous plate handling of the Orbitor RS Microplate Mover (Figure 10). The EFS uses a cube of Attune Focusing Fluid and a Nalgene waste bottle (Figure 11). The EFS is ideal for use with the Attune NxT Autosampler and enables the collection of approximately 28 96-well or 7 384-well plates without changing the fluid tanks.

This device was designed to facilitate the use of a larger buffer and waste containers. The EFS connects to the Attune NxT flow cytometer via umbilical cords (Figure 12). The system works by monitoring and supplying the internal fluid tanks on the Attune NxT instrument so that users don’t have to interrupt instrument operation to change out the fluid tanks or empty the waste.

The Attune NxT External Fluid Supply (EFS) device
Figure 11. The Attune NxT External Fluid Supply (EFS) device, an optional accessory to the Attune NxT flow cytometer, is equipped with a 10L cube of focusing fluid and a Nalgene waste bottle.
The Attune NxT External Fluid Supply (EFS) device

Figure 12. Umbilical tubing connects from the External Fluid Supply to the Attune NxT flow cytometer fluidics chamber.


Anchor your automation with proven reliability in operation and innovative mechanical integrity

Leverage the robotic automation integration workstation with a suite of Invitrogen and Thermo Scientific instruments for outstanding performance, walkaway reliability, accurate monitoring, and productive uptime. Optimize for particular specifications, workload, and objectives of multi-instrument, high-volume labs with custom upgrades.

  • Rich data—this degree of automation enables measurably more consistency
  • Coordinated operations—boost efficiency and trim production cycle intervals
  • Sustained reliability—mechanically engineered to achieve long lasting high-performance.

True walk-away automation is not achieved solely with the integration of robotics and defined points of user intervention but rather when every instrument in the workcell is reliable. A system’s speed is inconsequential if the system is not also robust in its operation. Choose expertly engineered instruments for your automation workcell.

Technical specifications: Thermo Scientific Orbitor RS Microplate Mover

Axis/Joint
  • (Height): 575 mm (Range); 750 mm/sec (Velocity); 2250 mm/sec2 (Acceleration)
  • Radius (Reach): ±406 mm (Range); 200 mm/sec (Velocity); 3600 mm/sec2 (Acceleration)
  • Theta (Rotation): Infinite (Range); 225 deg/sec (Velocity); 675 deg/sec2 (Acceleration)
Grip
  • Plate type:  Handles most SBS conforming plates
  • Maximum microplate weight: 250 g including sample
  • Orientation:  Landscape or Portrait
  • Lids: Placement and removal
  • Plate detection: Yes
  • Barcode reading: Integrated reader in grip; compatible with freestanding reader
  • Force and speed: User-adjustable
  • Fingers: Variable plate/container designs
Operation
  • Unattended Mode: Yes, with text and email error and completion notification settings
  • Overnight Operation settings: Yes
  • Error Event Escalation settings: User-definedignore, warning, or error
  • Admin settings: Assignable roles (Administrator, Operator, Expert)
  • Computers required: (A) Co-located on one unit with Momentum software executing local control of Attune NxT software; or (B) Installed on two separate computers with Momentum software executing remote control of Attune NxT software
  • Regulatory: Research Use Only
Safety
  • Collision Detection and Recovery: Self-homing mover
  • Manual Override: Hardwired emergency stop button
Physical Characteristics
  • Power requirements: 110-230 V auto switching
  • Operating environment: 4–40°C, non-condensing
  • Safety compliances: CE, CSA
  • Weight: 25 kg (55 lb)
  • Motion Control: Closed Loop Servo, Brushless AC Motor
  • Operator control: Directly from PC, requires Windows XP or Windows Vista operating systems
Software
  • Interface format: Dashboard
  • Runtime views: Detailed
  • Mid-run sample loading/unloading: Available
  • Worklist: Automatic execution enabled; import and barcode value matching
  • Process flow: User-set time and logical constraints
  • Shared lab: Multi-user login management
  • Process validation and version control: Built in
  • Compliance: Full xml audit log CFR21-11 & CLIA compliant
  • Distributed motion: Multi-mover systems supported
  • Workflow designer: Drag and drop
  • Sample tracking: By barcodes and sample ID
  • Container definition: Built-in library
  • Simulation mode: Realtime, Accelerated
  • Progress Visualization: Plate tracing and visual progress of projects
  • Priority: Active adjustable with dynamic scheduling
Requisite Environmental Conditions
  • Maximum operating temperature:  Ambient +10° to +40°C (50° to 104°F)
  • Storage temperature: 20° to 75°C (–4° to 167°F)
  • Ambient humidity: 10% to 80% RH non-condensing
  • Occupancy: Indoor use only
Power
  • Electrical requirements: 100–240 VAC, 50/60 Hz, nominal
  • Receptacle: Requires a NEMA 5-15 female receptacle
  • Consumption: 80 W maximum
Data Management
  • Software: Thermo Scientific Momentum Laboratory Automation Workflow Software
  • Application programming interface: Invitrogen Attune NxT Software 
  • Flow cytometer: Invitrogen Attune NxT Flow Cytometer 
  • CPU: 2 GHz or faster 32-bit (X86) or 64-bit (X64)
  • RAM: 2 GB (32-bit) or 3 GB (64-bit)
  • USB ports: 2
  • License key: Yes, USB
  • Operating System: Windows 7 Professional
Elevator, Reach and Axes

Clearance (H x W x D)

  • Relative positioning: Inline or opposed configuration. Orbitor mover installs adjacent to the plate nest side of the flow cytometer
  • Overall space required for Orbitor installation: 29” x 36” x 36”
  • Without arms extended: 14”
  • With arm extended: 24”
  • With storage: 24”
Microplate Storage
  • Random access: 8 plates per hotel
  • Sequential access: 40 plates per stack
  • Maximum standard hotel capacity: 72 plates
  • Maximum standard stack capacity: 360 plates
  • Expanded capacity: Custom spines available
  • Temperature control: SmartStor storage device available
Mounting

Supporting surface area

  • Bench: Flat and sufficiently stable to support robot at 50 lbs and storage at 20 lbs minimum
  • Mounting: Required 4x M6 Machine screws or 4x #12 wood screws

  • Technical Specifications: Thermo Scientific SmartStor Microplate Storage Device

    Temperature Range
    • Controlled range: 4 to 40°C
    Inventory
    • Standard microplate: 20
    • Deep well blocks: 9
    • Internal inventory monitoring: Scanner-determined inventory updates

    Technical Specifications: Attune NxT External Fluid Supply (EFS)

    Focusing Fluid
    • Focusing fluid container: 10 L cubetainer, single use
    • Focusing fluid connection: Cap with sensor and fluid intake
    • Umbilical 1: For focusing fluid cubetainer
    Waste
    • Umbilical 2: 6 ft connection
    • Maximum waste volume: up to 18 L (including 2 L of bleach for biosafety requirements)
    • Containment: 20 L reusable Nalgene standard container (non-autoclavable) with modified lid containing vent and sensor.
    • Full notification: Yes, alarm
    Physical Characteristics
    • H/D/W counter footprint: 10 in (25.4 cm) x 16.5 in (41.9 cm) x 25 in (63.5 cm)
    • H/D/W with containers: 22 in (55.9 cm) x 16.5 in (41.9 cm) x 25 in (63.5 cm)
    • Dry weight: Approximately 30 lb/13.6 kg
    • Weight with 10 L fluid: Approximately 62 lb/28.1 kg
    • Electrical input: From Attune NxT flow cytometer
    Operating Range
    • Operating range: 15°–32°C
    • Humidity range: 10–90% non-condensing

    Technical Specifications: Attune NxT Flow Cytometer

    Optics
    • Laser power (as shown in table below)
    Laser Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
    Violet 405 50 100
    Blue 488 50 100
    Green 532 100 140
    Yellow 561 50 100
    Red 637 100 140
    * Amount of measured usable laser power after light has gone through the beam optics and shaping filters.
    ** Vendor-specified theoretical maximum.
    • Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection
    • Laser profile: 10 x 50 μm flat-top laser providing robust alignment
    • Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters
    • Laser separation: 150 μm
    • Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance required
    • Onboard thermoelectric cooler: No warm-up delay; fiber isn’t affected by on/off
    • Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it “on” when acquiring samples; reports hours of usage
    • Flat top specified at the flow cell: Coefficient of variation (CV) <3% over width of flat top
    • Upgradable: Convenient field changes
    Fluids
    • Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
    • Sample analysis volume: 20 μL–4 mL
    • Custom sample flow rates: 12.5–1,000 μL/min
    • Sample delivery: Positive-displacement syringe pump for volumetric analysis
    • Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
    • Fluid-level sensing: Active
    • Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
    • Fluid storage: All fluids stored within instrument
    • Extended fluidics option: Configuration for 10 L fluid
    • Nominal fluid consumption: 1.8 L/day
    • Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
    Performance
    • Fluorescence sensitivity: ≤80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤30 MESF for PE, ≤70 MESF for APC
    • Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
    • Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
    • Maximum electronic speed: 65,000 events/sec with all parameters
    • Carryover: Single-tube format: <1%
    • Forward and side scatter sensitivity: Able to discriminate platelets from noise
    • Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
    • Forward scatter: Photodiode detector with 488/10 nm bandpass filter
    • Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
    • Fluorescent detectors: 14 individual detectors
    • Electronic pulse: Measured area, height, and width pulse for all detectors
    • Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
    • Minimum particle size: 0.2 μm on side scatter using Bangs Laboratories, Inc.™ Submicron Bead Calibration Kit (Cat. No. 832)

     Download full instrument specifications

    Learn more about the Attune NxT Flow Cytometer


    Technical Specifications: Attune NxT Autosampler

    Performance
    • High-throughput mode acquisition time: <42 min for 96-well plate, <180 min for 384-well plate (using one rinse and one mix, and full analysis of a 40 μL sample)
    • Carryover: <0.5% in plate loader format (standard mode, 2 wash cycles); multiple-rinse capability for ultralow carryover
    • Sample mixing: Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume
    • Mixing method: Each well mixed via full aspiration (no shaking)
    • Wash cycle: User-defined number of wash cycles, dependent on plate-processing protocol and time to acquire plates
    • Minimum dead volume (single draw): 30 μL for 12.5–200 μL/min, 50 μL for 50–1,000 μL/min
    • Sample window: Protectively coated window allows visibility to well progress while preventing exposure to ambient light during acquisition
    • Auto-calibration: Regular, 30-day interval, system-initiated function
    Fluidics
    • Plate and tube compatibility: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
    • Compatible plate types: 96 deep-well (flat, round, and V-bottom), 96-well standard depth (flat, round, and V-bottom), 384-well standard depth (flat, round, and V-bottom), 384 deep-well (flat, round, and V-bottom)
    • Cleaning cycles: Automated daily and monthly cleaning protocols
    • Fluidics requirements: 800 mL total of onboard fluid tanks, capable of running four 96-well plates
    • Extended fluidics option: Optional external fluid tank with 10 L fluid capacity

    Learn more about the Attune NxT Autosampler

    Momentum software

    Home screen

    The Orbitor RS and Attune NxT software are connected through Momentum Laboratory Automation Workflow Software. When automation is enabled, Momentum software is responsible for managing the process. Momentum software can be installed on the same computer as Attune NxT software, or Momentum software can be installed on a separate computer and control the Attune NxT software remotely.

    Achieve new levels of power, performance and ease with Thermo Scientific Momentum software—standardized real-time, data-driven decision making that eliminates customized data handling.

    User experience

    Momentum software helps enable flow cytometry workcells to accurately deliver the plate sampling necessary to your applications.

    • Define, execute and monitor complex processes
    • Add plates, choose pre-validated automated workflows and monitor the progress of ongoing work units in a powerful yet straightforward visual environment
    • Specify the design, configuration and operation using the open topology editor

    Worklists

    Import worklists and match barcode value to specific templates.

    Progress visualization

    Trace plates and visualize progress of projects relative to others in priority on the Momentum software dashboard.

    Multiuser, multiprocess scheduling

    Actively prioritize and reprioritize runs in high-volume labs with dynamic scheduling.

    User settings

    Customizable variables is SW settings can be set to align with lab operations. For example, monitor a specific fluid, modify a default setting, or create batch scope that is most relevant.

    Admin settings

    Assign user roles through the software:

    • An admin has all rights and manages user set up
    • Operators can run, but not modify
    • Experts assign operators and create new experiments and processes

    Unique software licensing model

    • All instrument drivers on one license key
    • Optional stay-current licensing package for updates

    Features

    • Real-time decision making
    • Error monitoring, recovery and control
    • Dynamic scheduling and rescheduling
    • Provides full audit trails and detailed error logging
    • Instrument pooling
    • Instrument hot-swapping
    • Ability to simulate and time a process run
    • Manual operation steps can be added to the workflow
    • Ability to unify multiple islands of automation treating them as part of a larger connected system

    Advanced functionality

    • Optional dashboard for user controls and monitoring
    • Ability to schedule variable-based decision steps
    • Add plates and new methods to a live running system without pausing
    • Design and edit process while system is running
    • Run multiple methods at the same time
    • File Watcher: system monitors for file-based worklists and executes flagged runs automatically

    User experience

    • Intuitive graphical drag and drop process editor

    Audit trails

    • Changes made to system (methods, parameters, user access levels etc.)
    • All runs started
    • All data collected
    • All instrument operations
    • All system events
    • All user events
    • All errors generated
    • All errors from which the system automatically recovered 

    Capable, well-educated and experienced Service and Support

    Invitrogen and Thermo Scientific instruments are supported by an extensive, experienced global team offering solutions to ensure that your automation systems are optimized for accuracy, productivity, and performance.

    Our premier service and support network options include:

    Integration and Relocation services

    Our Field Service Engineers are available for setting up or relocating a multicomponent workcell or adding additional instruments to the configuration.  

    Service and Support

    Field Service Engineers dispatch to service calls and manage all instrument installs. An extended service option also available. Preventative maintenance is another valuable aspect of your investment.

    Technical Support

    A tech support line is available with rolling time zone routing. Speak with an engineer or technician on the phone to answer questions and troubleshoot.

    Software application support

    Take advantage of the nimble adaptability of Momentum Laboratory Automation Workflow Software to configure operational settings that help enable your automated workcell to meet your needs. Our technical specialists are able to collaborate with you to establish and modify software applications and upgrades. 

    Application specialists

    Globally, dozens of PhD scientists build the ranks of our teams for supporting, developing and partnering with you to expand the complexity of your cell-based assays, determine the best instrumentation configuration for your applications and help you just get to the science.

    Training workshops

    Post-install, labs receive a 4-hour familiarization workshop to learn about operating the Attune NxT flow cytometer in concert with the Orbitor RS Microplate Mover, the External Fluid Supply and the SmartStor microplate storage device. Supplementary sessions are available for expanded operation and application optimization.

    Technical specifications: Thermo Scientific Orbitor RS Microplate Mover

    Axis/Joint
    • (Height): 575 mm (Range); 750 mm/sec (Velocity); 2250 mm/sec2 (Acceleration)
    • Radius (Reach): ±406 mm (Range); 200 mm/sec (Velocity); 3600 mm/sec2 (Acceleration)
    • Theta (Rotation): Infinite (Range); 225 deg/sec (Velocity); 675 deg/sec2 (Acceleration)
    Grip
    • Plate type:  Handles most SBS conforming plates
    • Maximum microplate weight: 250 g including sample
    • Orientation:  Landscape or Portrait
    • Lids: Placement and removal
    • Plate detection: Yes
    • Barcode reading: Integrated reader in grip; compatible with freestanding reader
    • Force and speed: User-adjustable
    • Fingers: Variable plate/container designs
    Operation
    • Unattended Mode: Yes, with text and email error and completion notification settings
    • Overnight Operation settings: Yes
    • Error Event Escalation settings: User-definedignore, warning, or error
    • Admin settings: Assignable roles (Administrator, Operator, Expert)
    • Computers required: (A) Co-located on one unit with Momentum software executing local control of Attune NxT software; or (B) Installed on two separate computers with Momentum software executing remote control of Attune NxT software
    • Regulatory: Research Use Only
    Safety
    • Collision Detection and Recovery: Self-homing mover
    • Manual Override: Hardwired emergency stop button
    Physical Characteristics
    • Power requirements: 110-230 V auto switching
    • Operating environment: 4–40°C, non-condensing
    • Safety compliances: CE, CSA
    • Weight: 25 kg (55 lb)
    • Motion Control: Closed Loop Servo, Brushless AC Motor
    • Operator control: Directly from PC, requires Windows XP or Windows Vista operating systems
    Software
    • Interface format: Dashboard
    • Runtime views: Detailed
    • Mid-run sample loading/unloading: Available
    • Worklist: Automatic execution enabled; import and barcode value matching
    • Process flow: User-set time and logical constraints
    • Shared lab: Multi-user login management
    • Process validation and version control: Built in
    • Compliance: Full xml audit log CFR21-11 & CLIA compliant
    • Distributed motion: Multi-mover systems supported
    • Workflow designer: Drag and drop
    • Sample tracking: By barcodes and sample ID
    • Container definition: Built-in library
    • Simulation mode: Realtime, Accelerated
    • Progress Visualization: Plate tracing and visual progress of projects
    • Priority: Active adjustable with dynamic scheduling
    Requisite Environmental Conditions
    • Maximum operating temperature:  Ambient +10° to +40°C (50° to 104°F)
    • Storage temperature: 20° to 75°C (–4° to 167°F)
    • Ambient humidity: 10% to 80% RH non-condensing
    • Occupancy: Indoor use only
    Power
    • Electrical requirements: 100–240 VAC, 50/60 Hz, nominal
    • Receptacle: Requires a NEMA 5-15 female receptacle
    • Consumption: 80 W maximum
    Data Management
    • Software: Thermo Scientific Momentum Laboratory Automation Workflow Software
    • Application programming interface: Invitrogen Attune NxT Software 
    • Flow cytometer: Invitrogen Attune NxT Flow Cytometer 
    • CPU: 2 GHz or faster 32-bit (X86) or 64-bit (X64)
    • RAM: 2 GB (32-bit) or 3 GB (64-bit)
    • USB ports: 2
    • License key: Yes, USB
    • Operating System: Windows 7 Professional
    Elevator, Reach and Axes

    Clearance (H x W x D)

    • Relative positioning: Inline or opposed configuration. Orbitor mover installs adjacent to the plate nest side of the flow cytometer
    • Overall space required for Orbitor installation: 29” x 36” x 36”
    • Without arms extended: 14”
    • With arm extended: 24”
    • With storage: 24”
    Microplate Storage
    • Random access: 8 plates per hotel
    • Sequential access: 40 plates per stack
    • Maximum standard hotel capacity: 72 plates
    • Maximum standard stack capacity: 360 plates
    • Expanded capacity: Custom spines available
    • Temperature control: SmartStor storage device available
    Mounting

    Supporting surface area

  • Bench: Flat and sufficiently stable to support robot at 50 lbs and storage at 20 lbs minimum
  • Mounting: Required 4x M6 Machine screws or 4x #12 wood screws

  • Technical Specifications: Thermo Scientific SmartStor Microplate Storage Device

    Temperature Range
    • Controlled range: 4 to 40°C
    Inventory
    • Standard microplate: 20
    • Deep well blocks: 9
    • Internal inventory monitoring: Scanner-determined inventory updates

    Technical Specifications: Attune NxT External Fluid Supply (EFS)

    Focusing Fluid
    • Focusing fluid container: 10 L cubetainer, single use
    • Focusing fluid connection: Cap with sensor and fluid intake
    • Umbilical 1: For focusing fluid cubetainer
    Waste
    • Umbilical 2: 6 ft connection
    • Maximum waste volume: up to 18 L (including 2 L of bleach for biosafety requirements)
    • Containment: 20 L reusable Nalgene standard container (non-autoclavable) with modified lid containing vent and sensor.
    • Full notification: Yes, alarm
    Physical Characteristics
    • H/D/W counter footprint: 10 in (25.4 cm) x 16.5 in (41.9 cm) x 25 in (63.5 cm)
    • H/D/W with containers: 22 in (55.9 cm) x 16.5 in (41.9 cm) x 25 in (63.5 cm)
    • Dry weight: Approximately 30 lb/13.6 kg
    • Weight with 10 L fluid: Approximately 62 lb/28.1 kg
    • Electrical input: From Attune NxT flow cytometer
    Operating Range
    • Operating range: 15°–32°C
    • Humidity range: 10–90% non-condensing

    Technical Specifications: Attune NxT Flow Cytometer

    Optics
    • Laser power (as shown in table below)
    Laser Wavelength (nm) Beam-shaping optics (BSO)* (mW) Diode power** (mW)
    Violet 405 50 100
    Blue 488 50 100
    Green 532 100 140
    Yellow 561 50 100
    Red 637 100 140
    * Amount of measured usable laser power after light has gone through the beam optics and shaping filters.
    ** Vendor-specified theoretical maximum.
    • Laser excitation: Optimized excitation for minimized stray laser-line noise and losses to reflection
    • Laser profile: 10 x 50 μm flat-top laser providing robust alignment
    • Emission filters: Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters
    • Laser separation: 150 μm
    • Optical alignment: Fixed alignment with prealigned welded fiber; no user maintenance required
    • Onboard thermoelectric cooler: No warm-up delay; fiber isn’t affected by on/off
    • Simmer mode: Instant on/off reduces usage and/or aging by 10x; only keep it “on” when acquiring samples; reports hours of usage
    • Flat top specified at the flow cell: Coefficient of variation (CV) <3% over width of flat top
    • Upgradable: Convenient field changes
    Fluids
    • Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm
    • Sample analysis volume: 20 μL–4 mL
    • Custom sample flow rates: 12.5–1,000 μL/min
    • Sample delivery: Positive-displacement syringe pump for volumetric analysis
    • Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
    • Fluid-level sensing: Active
    • Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
    • Fluid storage: All fluids stored within instrument
    • Extended fluidics option: Configuration for 10 L fluid
    • Nominal fluid consumption: 1.8 L/day
    • Automated maintenance cycles: ≤15 min startup and shutdown—deep clean, sanitize, and debubble modes
    Performance
    • Fluorescence sensitivity: ≤80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤30 MESF for PE, ≤70 MESF for APC
    • Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN)
    • Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics
    • Maximum electronic speed: 65,000 events/sec with all parameters
    • Carryover: Single-tube format: <1%
    • Forward and side scatter sensitivity: Able to discriminate platelets from noise
    • Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood
    • Forward scatter: Photodiode detector with 488/10 nm bandpass filter
    • Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter
    • Fluorescent detectors: 14 individual detectors
    • Electronic pulse: Measured area, height, and width pulse for all detectors
    • Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise
    • Minimum particle size: 0.2 μm on side scatter using Bangs Laboratories, Inc.™ Submicron Bead Calibration Kit (Cat. No. 832)

     Download full instrument specifications

    Learn more about the Attune NxT Flow Cytometer


    Technical Specifications: Attune NxT Autosampler

    Performance
    • High-throughput mode acquisition time: <42 min for 96-well plate, <180 min for 384-well plate (using one rinse and one mix, and full analysis of a 40 μL sample)
    • Carryover: <0.5% in plate loader format (standard mode, 2 wash cycles); multiple-rinse capability for ultralow carryover
    • Sample mixing: Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume
    • Mixing method: Each well mixed via full aspiration (no shaking)
    • Wash cycle: User-defined number of wash cycles, dependent on plate-processing protocol and time to acquire plates
    • Minimum dead volume (single draw): 30 μL for 12.5–200 μL/min, 50 μL for 50–1,000 μL/min
    • Sample window: Protectively coated window allows visibility to well progress while preventing exposure to ambient light during acquisition
    • Auto-calibration: Regular, 30-day interval, system-initiated function
    Fluidics
    • Plate and tube compatibility: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes
    • Compatible plate types: 96 deep-well (flat, round, and V-bottom), 96-well standard depth (flat, round, and V-bottom), 384-well standard depth (flat, round, and V-bottom), 384 deep-well (flat, round, and V-bottom)
    • Cleaning cycles: Automated daily and monthly cleaning protocols
    • Fluidics requirements: 800 mL total of onboard fluid tanks, capable of running four 96-well plates
    • Extended fluidics option: Optional external fluid tank with 10 L fluid capacity

    Learn more about the Attune NxT Autosampler

    Momentum software

    Home screen

    The Orbitor RS and Attune NxT software are connected through Momentum Laboratory Automation Workflow Software. When automation is enabled, Momentum software is responsible for managing the process. Momentum software can be installed on the same computer as Attune NxT software, or Momentum software can be installed on a separate computer and control the Attune NxT software remotely.

    Achieve new levels of power, performance and ease with Thermo Scientific Momentum software—standardized real-time, data-driven decision making that eliminates customized data handling.

    User experience

    Momentum software helps enable flow cytometry workcells to accurately deliver the plate sampling necessary to your applications.

    • Define, execute and monitor complex processes
    • Add plates, choose pre-validated automated workflows and monitor the progress of ongoing work units in a powerful yet straightforward visual environment
    • Specify the design, configuration and operation using the open topology editor

    Worklists

    Import worklists and match barcode value to specific templates.

    Progress visualization

    Trace plates and visualize progress of projects relative to others in priority on the Momentum software dashboard.

    Multiuser, multiprocess scheduling

    Actively prioritize and reprioritize runs in high-volume labs with dynamic scheduling.

    User settings

    Customizable variables is SW settings can be set to align with lab operations. For example, monitor a specific fluid, modify a default setting, or create batch scope that is most relevant.

    Admin settings

    Assign user roles through the software:

    • An admin has all rights and manages user set up
    • Operators can run, but not modify
    • Experts assign operators and create new experiments and processes

    Unique software licensing model

    • All instrument drivers on one license key
    • Optional stay-current licensing package for updates

    Features

    • Real-time decision making
    • Error monitoring, recovery and control
    • Dynamic scheduling and rescheduling
    • Provides full audit trails and detailed error logging
    • Instrument pooling
    • Instrument hot-swapping
    • Ability to simulate and time a process run
    • Manual operation steps can be added to the workflow
    • Ability to unify multiple islands of automation treating them as part of a larger connected system

    Advanced functionality

    • Optional dashboard for user controls and monitoring
    • Ability to schedule variable-based decision steps
    • Add plates and new methods to a live running system without pausing
    • Design and edit process while system is running
    • Run multiple methods at the same time
    • File Watcher: system monitors for file-based worklists and executes flagged runs automatically

    User experience

    • Intuitive graphical drag and drop process editor

    Audit trails

    • Changes made to system (methods, parameters, user access levels etc.)
    • All runs started
    • All data collected
    • All instrument operations
    • All system events
    • All user events
    • All errors generated
    • All errors from which the system automatically recovered 

    Capable, well-educated and experienced Service and Support

    Invitrogen and Thermo Scientific instruments are supported by an extensive, experienced global team offering solutions to ensure that your automation systems are optimized for accuracy, productivity, and performance.

    Our premier service and support network options include:

    Integration and Relocation services

    Our Field Service Engineers are available for setting up or relocating a multicomponent workcell or adding additional instruments to the configuration.  

    Service and Support

    Field Service Engineers dispatch to service calls and manage all instrument installs. An extended service option also available. Preventative maintenance is another valuable aspect of your investment.

    Technical Support

    A tech support line is available with rolling time zone routing. Speak with an engineer or technician on the phone to answer questions and troubleshoot.

    Software application support

    Take advantage of the nimble adaptability of Momentum Laboratory Automation Workflow Software to configure operational settings that help enable your automated workcell to meet your needs. Our technical specialists are able to collaborate with you to establish and modify software applications and upgrades. 

    Application specialists

    Globally, dozens of PhD scientists build the ranks of our teams for supporting, developing and partnering with you to expand the complexity of your cell-based assays, determine the best instrumentation configuration for your applications and help you just get to the science.

    Training workshops

    Post-install, labs receive a 4-hour familiarization workshop to learn about operating the Attune NxT flow cytometer in concert with the Orbitor RS Microplate Mover, the External Fluid Supply and the SmartStor microplate storage device. Supplementary sessions are available for expanded operation and application optimization.