Qdot 565 probe is an excellent orange-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~565 nm, Qdot 565 probe can be excited using a 350, 488, or 525 nm laser line or standard TRITC filter. Optimum imaging results with brighter signals are achieved with a Qdot 565 filter or light cube.
Made from nanocrystals of semiconductor material, Qdot probes are broadly compatible with standard fluorescence techniques, but there are some novel aspects to their use that can be optimized to obtain the best possible results. In general they have an extremely broad excitation range and narrow emission with a large Stokes shift. Optimal brightness is generally achieved using a specific Qdot filter.