Truncation Without Frameshifts
In a truncation library, the wild type sequence can be shortened from both ends in single amino acid/single codon steps.
GeneArt™ Truncation Libraries consist of a pool of DNA fragments of different length that all contain a certain core region of the gene of interest. Unlike other methods, our process preserves the open reading frame, avoiding out-of- frame mutations.
Truncation libraries can for example be used to:
- Optimize solubility
- Evaluate minimal functional-protein size
- Identify functional domains
- Screen for inhibitors
- Map epitopes