Dynabeads® Oligo(dT)25 can be applied directly to PCR (or any other enzymatic reactions) as the beads do not inhibit or reduce enzymatic activity. This is because the iron is safely sealed off inside the Dynabeads® by an inert polymer shell.
Adding the Dynabeads®-mRNA complex directly to the RT-PCR offers benefits: Elution, and thereby dilution, of the mRNA is avoided. With mRNA hybridized to the beads, the bead-bound oligo-dT act as primers for first strand cDNA synthesis, producing a bead-bound cDNA library. Construction of such a solid-phase cDNA library is of special benefit when mRNA is isolated from very small samples. Solid-phase cDNA libraries have been generated from a single cell using this technology. In addition, the solid-phase cDNA library can be regenerated and reused again and again, for almost unlimited number of times.