The next generation in
punch system technology

The Card Processing Automation 200 (CPA200™) Semi-Automated Puncher is designed to process single-source DNA samples on filter paper in forensic and paternity laboratories.

The CPA200™ instrument combines ease of use with full-sample traceability and integrity to improve downstream results in low- and medium-throughput laboratories.

This instrument represents the next generation of semi-automated punch systems, and is specifically designed to meet the needs of forensic and paternity laboratories routinely processing single-source samples collected on filter paper. The CPA200™ Semi-Automated Puncher is a benchtop instrument tailored to the needs of low- and medium-throughput laboratories interested in improving efficiency while reducing the risk of errors and maximizing chain of custody.

The CPA200™ instrument offers:

  • High punching accuracy to help ensure the disk is delivered in the correct well, and to minimize cross-contamination
  • Flexibility to process a variety of card types
  • Traceability of samples through barcode reading and camera control
  • Integration into laboratory standard operating procedures (SOPs) through laboratory information management systems (LIMS) and customizable exportable reports
Benefit
Punching accuracy
  • Ionizer to remove static
  • Vacuum system to remove debris from punching tool
  • Cleaning punch functionality
  • Laser targeting system the help the user select the best area for punching
System flexibility
  • Works with off-the-shelf and custom cards
  • Protocol optimized for the NUCLEIC-CARD™ DNA Collection System with dedicated cleaning area to improve efficiency and help reduce time to results
Full traceability
  • Plate and card barcode reading
  • Camera control of each well before and after punching
  • Real-time updates on plate status
  • Customizable reports
Specifications and features*

Supported card formats

All card formats are supported.
The CPA200™ instrument works with sample collection systems routinely used in forensic laboratories, including FTA® Cards (Whatman) and Buccal DNA Collector devices (Bode), and with both indicating cards (saliva samples) and nonindicating cards (blood samples).

Possibility to reprocess cards that have already been punched.
Optimized protocol

Using NUCLEIC-CARD™ framed card with cleaning area:
1-spot format: 51 mm x 51 mm

2-spot format: 85 mm x 53 mm

Available punch sizes

The CPA200™ instrument has the following standard punch configurations:
1.2 mm*
2 mm
3 mm
4 mm

*All CPA 200™ instruments come with 1.2 mm punch sizes by default.

Sample punching position

Framed cards: the user selects the punching position using the external laser pointer. The system will record the coordinates and automatically punch in the selected position.

All other cards: The user selects the punching position using the internal laser pointer aimed at the punching site.

Cleaning procedure

Optimized protocol using NUCLEIC-CARD™ framed  card with cleaning area: the first cleaning punch is performed automatically; possibility to repeat for the same cleaning area using the external laser pointer.

All other cards: cleaning procedure can be performed using a cleaning card or on a blank area of the processing card using the external or the internal laser pointer.
Capacity 2 plates (192 cards)

Camera features and imaging

Model: IDS Direct XS
Resolution: 2,592 (h) x 1,944 (v)
Sample presence control: Automatic comparison between the well picture before punching (well normally empty) and well picture after punching (well normally full).

Image storage: All acquired images are saved into a specific directory on the PC desktop.
Physical dimensions 610 mm (L) x 545 mm (W) x 675 mm (H); weight: 72 kg

Hardware and software

Model: ASEM HT2000-TF/S1: LCD TFT 10.4” 4:3, 10.4” touch-screen, Intel® Atom™ dual core D2550, 1,86 GHz, 1 GB RAM. Operating system: Ubuntu® Linux.
Peripherals: 10.4’’ touch screen, keyboard, mouse, USB (2)
Communication: USB

Network: 2 x Ethernet (1 Gb/s)
Exportable files The instrument can export files in formats suitable for the 3130 and 3500 Genetic Analyzer sequencers, and can export reports as PDF files.
Main power requirements 400 W (peak) electrical power; 208–240 VAC main supply voltage; frequency 50/60 Hz
Pollution degree 2
Overvoltage category 2

* Refer to CPA200™ instrument technical documents for additional information on specifications and validated protocols.

Specifications and features2

Supported card formats

The CPA300™ instrument works with framed NUCLEIC-CARD™ (COPAN) and FTA® Cards (Whatman) sample collection systems routinely used in forensic laboratories, and both indicating cards (saliva samples) and non-indicating cards (blood samples).
The CPA300™ instrument can reprocess already punched cards.

Optimized protocol

Using the framed NUCLEIC-CARD™ with cleaning area:
1-spot format: 51 mm x 51 mm

2-spot format: 85 mm x 53 mm

Available punch sizes

The CPA300™ instrument has the following standard punch configurations:
1.2 mm*
2 mm
3 mm
4 mm

*All CPA 300™ instruments come with the 1.2 mm punch size by default.

Sample detection

Automatic sample detection (saliva or blood)

Cleaning procedure

Optimized protocol using the NUCLEIC-CARD™ with integrated cleaning area.
Multiple cleaning punches on the same card.

Capacity Fully-automated: 3 plates (288 cards)

Camera features and imaging

Model: Allied Vision Technologies Manta
Resolution: 2452 (h) x 2056 (v)
Sample presence control: Automatic comparison between the well picture before punching (well normally empty) and after punching (well normally full). Verification of the empty/full well before proceeding with the next punching.

Image storage: All acquired images are saved to a specific directory on the PC desktop.
Physical dimensions Including PC: 1,700 mm (L) x 760 mm (W) x 1,005 mm (H)
Operational box: 1,160 mm (L) x 760 mm (W) x 1,005 mm (H)
Weight: 270 kg

PC Hardware and software

The computer is an industrial PC embedded inside the instrument: only the 19"
touch screen, mouse, and keyboard are visible to the user. The computer has a dual
core 2.2 GHz CPU, 4GB RAM and a 32 GB solid state drive. It runs on the Linux® Ubuntu® operating system.
Peripherals: 19'' touch screen/keyboard/mouse/USB (2).
Communication: USB/Data transfer:
Network: Ethernet (1 Gb/s)

LIMS Integration The instrument can be connected to LIMS in order to import and export files
Exportable files
The instrument can export files in formats suitable for the 3130 and 3500 Genetic Analyzer sequencers, and can export reports as PDF files.
Main power requirements 1200W (peak) electrical power; 110-240VAC main supply voltage; 50/60 Hz frequency
Pollution degree 2
Overvoltage category 2
Pipetting Unit 1 pipetting channel
Tip sizes: 50µL, 200µL, 1000µL
Tip station: dedicated area for 3 rack tips
Reagent station: 4 slots with optimized temperature settings

2 Refer to CPA300™ technical documents for additional information on specifications and validated protocols. A version of the CPA300™ without the sealing unit is available.