This table illustrates the reactivity of our Z´-LYTE Peptide Substrates using a broad range of kinases we have tested at Invitrogen. For each kinase in the left hand column, we list the Z´-LYTE substrate(s) that are recommended in the Z´-LYTE assay. We have substrates representing two classes of kinases, Tyrosine Kinase substrates and Serine/Theonine Kinase substrates. The number in the table refers to the substrate number of the Z’-LYTE Detection Assay Kit within each class of kinases.

View detailed assay conditions for each kinase used by our SelectScreen services team by visiting this page, and navigating to the Technology Overview section.

Tyr Kinase Z'-LYTE Assay Kit Assay Protocol
ABL1 Z'LYTE Kit-Tyr 02 Peptide Protocol
ABL1 E255K Z'LYTE Kit-Tyr 02 Peptide Protocol
ABL1 G250E Z'LYTE Kit-Tyr 02 Peptide Protocol
ABL1 T315I Z'LYTE Kit-Tyr 02 Peptide Protocol
ABL1 Y253F Z'LYTE Kit-Tyr 02 Peptide Protocol
ABL2 (ARG) Z'LYTE Kit-Tyr 02 Peptide Protocol
AXL Z'LYTE Kit-Tyr 06 Peptide Protocol
BLK Z'LYTE Kit-Tyr 01 Peptide Protocol
BMX Z'LYTE Kit-Tyr 01 Peptide Protocol
BTK Z'LYTE Kit-Tyr 01 Peptide Protocol
CSF1R (FMS) Z'LYTE Kit-Tyr 01 Peptide Protocol
CSK Z'LYTE Kit-Tyr 02 Peptide Protocol
EGFR (ERBB1) Z'LYTE Kit-Tyr 04 Peptide Protocol
EGFR (ERBB1) L858R Z'LYTE Kit-Tyr 04 Peptide Protocol
EGFR (ERBB1) L861Q Z'LYTE Kit-Tyr 04 Peptide Protocol
EGFR T790M Z'LYTE Kit-Tyr 04 Peptide Protocol
EGFR T790M L858R Z'LYTE Kit-Tyr 04 Peptide Protocol
EPHA1 Z'LYTE Kit-Tyr 02 Peptide Protocol
EPHA2 Z'LYTE Kit-Tyr 01 Peptide Protocol
EPHA4 Z'LYTE Kit-Tyr 02 Peptide Protocol
EPHA5 Z'LYTE Kit-Tyr 01 Peptide Protocol
EPHA8 Z'LYTE Kit-Tyr 02 Peptide Protocol
EPHB1 Z'LYTE Kit-Tyr 02 Peptide Protocol
EPHB2 Z'LYTE Kit-Tyr 02 Peptide Protocol
EPHB3 Z'LYTE Kit-Tyr 02 Peptide Protocol
EPHB4 Z'LYTE Kit-Tyr 01 Peptide Protocol
ERBB2 (HER2) Z'LYTE Kit-Tyr 06 Peptide Protocol
ERBB4 (HER4) Z'LYTE Kit-Tyr 01 Peptide Protocol
FER Z'LYTE Kit-Tyr 05 Peptide Protocol
FES (FPS) Z'LYTE Kit-Tyr 01 Peptide Protocol
FGFR1 Z'LYTE Kit-Tyr 04 Peptide Protocol
FGFR2 Z'LYTE Kit-Tyr 04 Peptide Protocol
FGFR3 Z'LYTE Kit-Tyr 04 Peptide Protocol
FGFR3 K650E Z'LYTE Kit-Tyr 04 Peptide Protocol
FGFR4 Z'LYTE Kit-Tyr 04 Peptide Protocol
FGR Z'LYTE Kit-Tyr 02 Peptide Protocol
FLT1 (VEGFR1) Z'LYTE Kit-Tyr 04 Peptide Protocol
FLT3 Z'LYTE Kit-Tyr 02 Peptide Protocol
FLT3 D835Y Z'LYTE Kit-Tyr 02 Peptide Protocol
FLT4 (VEGFR3) Z'LYTE Kit-Tyr 04 Peptide Protocol
FRK (PTK5) Z'LYTE Kit-Tyr 01 Peptide Protocol
FYN Z'LYTE Kit-Tyr 02 Peptide Protocol
HCK Z'LYTE Kit-Tyr 02 Peptide Protocol
IGF1R Z'LYTE Kit-Tyr 01 Peptide Protocol
INSR Z'LYTE Kit-Tyr 01 Peptide Protocol
INSRR (IRR) Z'LYTE Kit-Tyr 04 Peptide Protocol
ITK Z'LYTE Kit-Tyr 01 Peptide Protocol
JAK1 Z'LYTE Kit-Tyr 06 Peptide Protocol
JAK2 Z'LYTE Kit-Tyr 06 Peptide Protocol
JAK2 JH1 JH2 Z'LYTE Kit-Tyr 06 Peptide Protocol
JAK2 JH1 JH2 V617F Z'LYTE Kit-Tyr 06 Peptide Protocol
JAK3 Z'LYTE Kit-Tyr 06 Peptide Protocol
KDR (VEGFR2) Z'LYTE Kit-Tyr 01 Peptide Protocol
KIT Z'LYTE Kit-Tyr 06 Peptide Protocol
KIT T670I Z'LYTE Kit-Tyr 06 Peptide Protocol
LCK Z'LYTE Kit-Tyr 02 Peptide Protocol
LTK (TYK1) Z'LYTE Kit-Tyr 06 Peptide Protocol
LYN A Z'LYTE Kit-Tyr 02 Peptide Protocol
LYN B Z'LYTE Kit-Tyr 02 Peptide Protocol
MATK (HYL) Z'LYTE Kit-Tyr 01 Peptide Protocol
MERTK (CMER) Z'LYTE Kit-Tyr 02 Peptide Protocol
MET (CMET) Z'LYTE Kit-Tyr 06 Peptide Protocol
MET M1250T Z'LYTE Kit-Tyr 06 Peptide Protocol
MST1R (RON) Z'LYTE Kit-Tyr 06 Peptide Protocol
MUSK Z'LYTE Kit-Tyr 04 Peptide Protocol
NTRK1 (TRKA) Z'LYTE Kit-Tyr 01 Peptide Protocol
NTRK2 (TRKB) Z'LYTE Kit-Tyr 01 Peptide Protocol
NTRK3 (TRKC) Z'LYTE Kit-Tyr 01 Peptide Protocol
PDGFRA (PDGFR ALPHA) Z'LYTE Kit-Tyr 04 Peptide Protocol
PDGFRA D842V Z'LYTE Kit-Tyr 04 Peptide Protocol
PDGFRA T674I Z'LYTE Kit-Tyr 04 Peptide Protocol
PDGFRA V561D Z'LYTE Kit-Tyr 04 Peptide Protocol
PDGFRB (PDGFR BETA) Z'LYTE Kit-Tyr 04 Peptide Protocol
PTK2 (FAK) Z'LYTE Kit-Tyr 07 Peptide Protocol
PTK2B (FAK2) Z'LYTE Kit-Tyr 01 Peptide Protocol
PTK6 (BRK) Z'LYTE Kit-Tyr 01 Peptide Protocol
RET Z'LYTE Kit-Tyr 02 Peptide Protocol
RET V804L Z'LYTE Kit-Tyr 02 Peptide Protocol
RET Y791F Z'LYTE Kit-Tyr 02 Peptide Protocol
ROS1 Z'LYTE Kit-Tyr 01 Peptide Protocol
SRC Z'LYTE Kit-Tyr 02 Peptide Protocol
SRC N1 Z'LYTE Kit-Tyr 02 Peptide Protocol
SRMS (SRM) Z'LYTE Kit-Tyr 01 Peptide Protocol
SYK Z'LYTE Kit-Tyr 02 Peptide Protocol
TEK (TIE2) Z'LYTE Kit-Tyr 05 Peptide Protocol
TXK Z'LYTE Kit-Tyr 06 Peptide Protocol
TYK2 Z'LYTE Kit-Tyr 03 Peptide Protocol
TYRO3 (RSE) Z'LYTE Kit-Tyr 02 Peptide Protocol
YES1 Z'LYTE Kit-Tyr 02 Peptide Protocol
ZAP70 Z'LYTE Kit-Tyr 07 Peptide Protocol
Ser/Thr Kinase Z'-LYTE Assay Kit Assay Protocol
ACVR1B (ALK4) Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
ADRBK1 (GRK2) Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
ADRBK2 (GRK3) Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
AKT1 (PKB ALPHA) Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
AKT2 (PKB BETA) Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
AKT3 (PKB GAMMA) Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
AMPK A1/B1/G1 Z'LYTE Kit-Ser/Thr 23 Peptide Protocol
AMPK A2/B1/G1 Z'LYTE Kit-Ser/Thr 23 Peptide Protocol
AURKA (AURORA A) Z'LYTE Kit-Ser/Thr 01 Peptide Protocol
AURKB (AURORA B) Z'LYTE Kit-Ser/Thr 01 Peptide Protocol
AURKC (AURORA C) Z'LYTE Kit-Ser/Thr 19 Peptide Protocol
BRAF Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
BRAF V559E Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
BRSK1 (SAD1) Z'LYTE Kit-Ser/Thr 21 Peptide Protocol
CAMK1D (CAMKI DELTA) Z'LYTE Kit-Ser/Thr 25 Peptide Protocol
CAMK2A (CAMKII ALPHA) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
CAMK2B (CAMKII BETA) Z'LYTE Kit-Ser/Thr 17 Peptide Protocol
CAMK2D (CAMKII DELTA) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
CAMK4 (CAMKIV) Z'LYTE Kit-Ser/Thr 13 Peptide Protocol
CDC42 BPB (MRCKB) Z'LYTE Kit-Ser/Thr 13 Peptide Protocol
CDC42BPA (MRCKA) Z'LYTE Kit-Ser/Thr 13 Peptide Protocol
CDK1/CYCLIN B Z'LYTE Kit-Ser/Thr 18 Peptide Protocol
CDK2/CYCLIN A Z'LYTE Kit-Ser/Thr 12 Peptide Protocol
CDK5/P25 Z'LYTE Kit-Ser/Thr 12 Peptide Protocol
CDK5/P35 Z'LYTE Kit-Ser/Thr 12 Peptide Protocol
CHEK1 (CHK1) Z'LYTE Kit-Ser/Thr 19 Peptide Protocol
CHEK2 (CHK2) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
CLK1 Z'LYTE Kit-Ser/Thr 09 Peptide Protocol
CLK2 Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
CLK3 Z'LYTE Kit-Ser/Thr 18 Peptide Protocol
CSNK1A1 (CK1 ALPHA1) Z'LYTE Kit-Ser/Thr 11 Peptide Protocol
CSNK1D (CK1 DELTA) Z'LYTE Kit-Ser/Thr 11 Peptide Protocol
CSNK1E (CK1 EPSILON) Z'LYTE Kit-Ser/Thr 11 Peptide Protocol
CSNK1G1 (CK1 GAMMA 1) Z'LYTE Kit-Ser/Thr 05 Peptide Protocol
CSNK1G2 (CK1 GAMMA 2) Z'LYTE Kit-Ser/Thr 05 Peptide Protocol
CSNK1G3 (CK1 GAMMA 3) Z'LYTE Kit-Ser/Thr 05 Peptide Protocol
CSNK2A1 (CK2 ALPHA 1) Z'LYTE Kit-Ser/Thr 11 Peptide Protocol
CSNK2A2 (CK2 ALPHA 2) Z'LYTE Kit-Ser/Thr 11 Peptide Protocol
DAPK3 (ZIPK) Z'LYTE Kit-Ser/Thr 13 Peptide Protocol
DCAMKL2 (DCK2) Z'LYTE Kit-Ser/Thr 17 Peptide Protocol
DYRK1A Z'LYTE Kit-Ser/Thr 18 Peptide Protocol
DYRK1B Z'LYTE Kit-Ser/Thr 18 Peptide Protocol
DYRK3 Z'LYTE Kit-Ser/Thr 09 Peptide Protocol
DYRK4 Z'LYTE Kit-Ser/Thr 09 Peptide Protocol
EEF2K Z'LYTE Kit-Ser/Thr 24 Peptide Protocol
FRAP1 (MTOR) Z'LYTE Kit-Ser/Thr 11 Peptide Protocol
GRK4 Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
GRK5 Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
GRK6 Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
GRK7 Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
GSK3A Z'LYTE Kit-Ser/Thr 09 Peptide Protocol
GSK3B (GSK3 BETA) Z'LYTE Kit-Ser/Thr 09 Peptide Protocol
HIPK1 (MYAK) Z'LYTE Kit-Ser/Thr 09 Peptide Protocol
HIPK2 Z'LYTE Kit-Ser/Thr 09 Peptide Protocol
HIPK3 (YAK1) Z'LYTE Kit-Ser/Thr 18 Peptide Protocol
HIPK4 Z'LYTE Kit-Ser/Thr 18 Peptide Protocol
IKBKB (IKK BETA) Z'LYTE Kit-Ser/Thr 05 Peptide Protocol
IKBKE (IKK EPSILON) Z'LYTE Kit-Ser/Thr 11 Peptide Protocol
IRAK4 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
MAP2K1 (MEK1) Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
MAP2K2 (MEK2) Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
MAP2K6 (MKK6) Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
MAP3K8 (COT) Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
MAP3K9 (MLK1) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
MAP4K2 (GCK) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
MAP4K4 (HGK) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
MAP4K5 (KHS1) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
MAPK1 (ERK2) Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
MAPK10 (JNK3) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
MAPK11 (P38 BETA) Z'LYTE Kit-Ser/Thr 15 Peptide Protocol
MAPK12 (P38 GAMMA) Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
MAPK13 (P38 DELTA) Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
MAPK14 (P38 ALPHA) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
MAPK3 (ERK1) Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
MAPK8 (JNK1) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
MAPK9 (JNK2) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
MAPKAPK2 Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
MAPKAPK3 Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
MAPKAPK5 (PRAK) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
MARK1 (MARK) Z'LYTE Kit-Ser/Thr 21 Peptide Protocol
MARK2 Z'LYTE Kit-Ser/Thr 21 Peptide Protocol
MARK3 Z'LYTE Kit-Ser/Thr 25 Peptide Protocol
MARK4 Z'LYTE Kit-Ser/Thr 25 Peptide Protocol
MELK Z'LYTE Kit-Ser/Thr 17 Peptide Protocol
MINK1 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
MKNK1 (MNK1) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
MST4 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
MYLK2 (SKMLCK) Z'LYTE Kit-Ser/Thr 13 Peptide Protocol
NEK1 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
NEK2 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
NEK4 Z'LYTE Kit-Ser/Thr 17 Peptide Protocol
NEK6 Z'LYTE Kit-Ser/Thr 22 Peptide Protocol
NEK7 Z'LYTE Kit-Ser/Thr 22 Peptide Protocol
NEK9 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PAK1 Z'LYTE Kit-Ser/Thr 19 Peptide Protocol
PAK2 (PAK65) Z'LYTE Kit-Ser/Thr 20 Peptide Protocol
PAK3 Z'LYTE Kit-Ser/Thr 20 Peptide Protocol
PAK4 Z'LYTE Kit-Ser/Thr 20 Peptide Protocol
PAK6 Z'LYTE Kit-Ser/Thr 20 Peptide Protocol
PAK7 (KIAA1264) Z'LYTE Kit-Ser/Thr 20 Peptide Protocol
PASK Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PDK1 Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
PHKG1 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PHKG2 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PIM1 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PIM2 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PKN1 (PRK1) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PLK1 Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
PLK2 Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
PLK3 Z'LYTE Kit-Ser/Thr 16 Peptide Protocol
PRKACA (PKA) Z'LYTE Kit-Ser/Thr 01 Peptide Protocol
PRKCB2 (PKC BETA II) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PRKCN (PKD3) Z'LYTE Kit-Ser/Thr 17 Peptide Protocol
PRKCQ (PKC THETA) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
PRKD1 (PKC MU) Z'LYTE Kit-Ser/Thr 17 Peptide Protocol
PRKD2 (PKD2) Z'LYTE Kit-Ser/Thr 17 Peptide Protocol
PRKG1 Z'LYTE Kit-Ser/Thr 01 Peptide Protocol
PRKG2 (PKG2) Z'LYTE Kit-Ser/Thr 01 Peptide Protocol
PRKX Z'LYTE Kit-Ser/Thr 01 Peptide Protocol
RAF1 (CRAF) Y340D Y341D Z'LYTE Kit-Ser/Thr 03 Peptide Protocol
ROCK1 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
ROCK2 Z'LYTE Kit-Ser/Thr 13 Peptide Protocol
RPS6KA1 (RSK1) Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
RPS6KA2 (RSK3) Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
RPS6KA3 (RSK2) Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
RPS6KA4 (MSK2) Z'LYTE Kit-Ser/Thr 01 Peptide Protocol
RPS6KA5 (MSK1) Z'LYTE Kit-Ser/Thr 01 Peptide Protocol
RPS6KA6 (RSK4) Z'LYTE Kit-Ser/Thr 20 Peptide Protocol
RPS6KB1 (P70S6K) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
SGK (SGK1) Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
SGK2 Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
SGK3 (SGKL) Z'LYTE Kit-Ser/Thr 06 Peptide Protocol
SNF1LK2 (QIK) Z'LYTE Kit-Ser/Thr 25 Peptide Protocol
SRPK1 Z'LYTE Kit-Ser/Thr 18 Peptide Protocol
SRPK2 Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
STK22B (TSSK2) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
STK22D (TSSK1) Z'LYTE Kit-Ser/Thr 04 Peptide Protocol
STK23 (MSSK1) Z'LYTE Kit-Ser/Thr 18 Peptide Protocol
STK24 (MST3) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
STK25 (YSK1) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
STK3 (MST2) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
STK4 (MST1) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
TAOK2 (TAO1) Z'LYTE Kit-Ser/Thr 07 Peptide Protocol
TBK1 Z'LYTE Kit-Ser/Thr 05 Peptide Protocol