Gamma tubulin is ubiquitously expressed in eukaryotic cells, so it is often used as a loading control. It is an essential protein for cell growth and division, as it organizes microtubule arrays in the nucleus and cytoplasm.

Gamma tubulin is found primarily in centrosomes and spindle pole bodies where the most abundant microtubule nucleation occurs. In these microtubule organizing centers, gamma tubulin associates with several other proteins to form the gamma tubulin ring complex which provides scaffolding for alpha/beta tubulin dimers to begin polymerization. It also provides a cap that allows the microtubule to grow in the (+) direction.

Quality Invitrogen gamma tubulin antibodies are available for a variety of research  needs, and most have been extensively validated for western blotting and immunostaining controls.

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Immunohistochemistry on normal deparaffinized human colon tissue. To expose target proteins, heat-induced antigen retrieval was performed using 10 mM sodium citrate (pH 6.0) buffer, microwaved for 8–15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a gamma tubulin mouse monoclonal antibody (Cat. No. MA1-850) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.



Western blot analysis of gamma tubulin using various whole cell extracts (30 µg). Samples were electrophoresed in a 10% SDS-PAGE gel, transferred to membrane, and probed with a gamma tubulin polyclonal antibody (Cat. No. PA5-34815) at a dilution of 1:1,000.

Annotated product references

Cat. No. MA1-850 was used in immunohistochemistry to investigate the neuroprotective effect of synphilin-1 in the A53T alpha-synuclein transgenic mouse model. Human molecular genetics (Jun 2010; 19: 2087) "Synphilin-1 attenuates neuronal degeneration in the A53T alpha-synuclein transgenic mouse model." Smith WW,Liu Z,Liang Y,Masuda N,Swing DA,Jenkins NA,Copeland NG,Troncoso JC,Pletnikov M,Dawson TM,Martin LJ,Moran TH,Lee MK,Borchelt DR,Ross CA

Cat. No. MA1-850 was used in immunocytochemistry and western blot to identify the role of TaSP in microtubule attachment of microbial pathogen. Parasitology research (Apr 2010; 106: 1085) "Schizonts of Theileria annulata interact with the microtubuli network of their host cell via the membrane protein TaSP." Seitzer U,Gerber S,Beyer D,Dobschanski J,Kullmann B,Haller D,Ahmed JS