Your choice for optimal scale-up

Start your scale-up with a T-25 flask that has the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes straight-neck or angled-neck options and surface treatment alternatives that help to ensure your healthy tissue cells expand and grow in the flask of your choice.

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What makes our Nunc EasYFlasks so easy to use?

Angled neck
 

Angled neck

EasYFlasks

  • Ergonomic design for easy access to the growth surface by pipettes or cell scrapers
  • Low profile to maximize incubator space
  • Slightly angled side walls for complete viewing under a microscope
  • Volume graduations printed and molded on both sides
  • Sterilized by gamma irradiation and certified to be non-pyrogenic

Filter or vent/close cap
 

Filter caps

  • Effective barrier against micro-organisms
  • Continuous venting with consistent gas exchange

Vent/close caps

  • “Y” mark allows visual verification of vent position: open or closed

Find your T25 cell culture flasks by surface

To help ensure flexible, reproducible and reliable results across every stage of cell culture, we offer a range of cell culture flasks spanning a variety of formats, sizes and surfaces. Let us help guide your product selection with specific cell types and desired cell culture ranges in mind.

For additional guidance on usage by cell type, view our Surface Selection Guide (PDF).

Adherent cell culture vs. suspension cell culture
There are two basic systems for growing cells in culture, as monolayers on an artificial substrate (i.e., adherent culture) or free-floating in the culture medium (suspension culture). Visit our technical reference library for an introduction to cell culture and to take a closer look at adherent cell culture vs. suspension cell culture.

The Thermo Scientific™ Nunclon™ Delta cell culture surface
The Nunclon Delta surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. The Nunclon Delta certificate ensures high quality standards and lot-to-lot consistency. Look for it on a variety of Thermo Scientific™ cell culture plastics. 

T25 flasks for adherent cells

The proprietary Nunclon Delta cell culture surface supports optimal growth and attachment of cells to the growth surface.

  Neck Surface Material Cap Cap feature
EasYFlask Angled Nunclon Delta Cell culture-treated polystyrene Filter Constant airflow
EasYFlask Angled Nunclon Delta Cell culture-treated polystyrene Vent/Close Sufficient gas exchange

     


We also have standard Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with angled necks and filtered caps or vent/close caps.


T25 flasks for spheroid culture, organoid culture, and 3D culture

The ultra low-binding surface is designed to grow consistent, reproducible spheroid and organoid cultures; ideally suited for cancer and stem cells.

Make your most valuable work your best work with Thermo Scientific™ Nunclon™ Sphera

Elevate your stem cell and cancer research with a surface that supports many different cell types and their ability to generate spheroids. Studies show that the Nunclon Sphera surface enables consistent, repeatable spheroid formation and growth. 

Request a sample

  Neck Surface Description Cap
Nunclon Sphera Flask Angled Nunclon Sphera Set in the EasYFlask format, the Nunclon Sphera Flasks enable 3D scaffold-free spheroid culture (e.g. tumor spheroids, embryoid body formation of pluripotent stem cells) Filter

T25 flasks with poly-d-lysine coating or collagen I coating

Poly-D-Lysine (PDL) and Collagen I coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface. Selection of one surface over another depends on tissue type; we suggest reviewing previous references for your specific cell line or testing both, if necessary.

  Neck Surface Description Cap
PDL-Coated EasYFlask Angled Poly-D-lysine Animal-free synthetic surface for
adherent cultures or primary cells
that are finicky and hard to attach
Filter
Collagen I-Coated EasYFlask Angled Collagen I Surface of animal origin; for
adherent cultures of primary cells
that are finicky and hard to attach
Filter

T25 flasks for suspension culture of non-adherent cells

Made with high quality, optically clear virgin polystyrene, the Thermo Scientific™ Nunc™ Non-treated Flasks, with high level sterility assurance, are ideal for suspension culture of non-adherent cells.

  Neck Surface Description Cap
Non-treated EasYFlask Angled Not cell culture treated Untreated polystyrene for suspension
growth of non adherent cells
Filter

Related flask and cell culture resources

Surface selection: Achieving optimal cell growth and harvesting

Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.

  • Surface modifications to improve cell adhesion
  • Characterization of Nunc cell culture surfaces by adhesion properties
  • Alternative methods for advanced cell harvesting

Watch now

Harnessing new dimensions in your research: Growing 3D cultures consistently and repeatedly

Learn about a novel surface technology that enables consistent, repeatable growth of spheroid cultures across different cell types.

  • Closely simulate tumor progression in vitro
  • Assess efficacy of anti-cancer agents
  • Generate embryoid bodies for germ layer differentiation
  • Form 3D cellular structures for many cell types

Watch now


Watch more cell culture webinars

Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.

Visit the café

Featured article

Cell adhesion and growth on coated or modified glass or plastic surfaces

Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge. 

Download bulletin

Are you on the front lines of cancer research?
 

Surface selection: Achieving optimal cell growth and harvesting

Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.

  • Surface modifications to improve cell adhesion
  • Characterization of Nunc cell culture surfaces by adhesion properties
  • Alternative methods for advanced cell harvesting

Watch now

Harnessing new dimensions in your research: Growing 3D cultures consistently and repeatedly

Learn about a novel surface technology that enables consistent, repeatable growth of spheroid cultures across different cell types.

  • Closely simulate tumor progression in vitro
  • Assess efficacy of anti-cancer agents
  • Generate embryoid bodies for germ layer differentiation
  • Form 3D cellular structures for many cell types

Watch now


Watch more cell culture webinars

Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.

Visit the café

Featured article

Cell adhesion and growth on coated or modified glass or plastic surfaces

Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge. 

Download bulletin

Are you on the front lines of cancer research?