Let your colors show with iBright Imaging Systems

iBright Imaging Systems feature up to four imaging modes, for capture of data from protein gels, nucleic acid gels, chemiluminescent western blots, and fluorescent western blots. Get the most from your investment by having the freedom to choose from multiple detection strategies to meet your experimental needs (Table 1).

Table 1. Overview of the types of data that can be captured from gels and blots.

Imaging capability What kind of signal can be captured?
Protein gel Colorimetric staining of gels (e.g., Coomassie dye, silver) and membranes (e.g., Ponceau S, Thermo Scientific MemCode stain)
Nucleic acid gel Ethidium bromide and Invitrogen SYBR dye staining
Chemiluminescent blot Chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates)
Fluorescent blot* Fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor, Alexa Fluor Plus, Thermo Scientific DyLight dyes)
*FL1000 model only.

With up to five fluorescent channels, many fluorophore options are available so that you can work with your preferred reagents. Up to 4-channel multiplexing is possible, with fluorophores in both the visible (RGB) fluorescent range and the near-infrared (near-IR) range. Table 2 shows the available fluorescence channels on iBright Imaging Systems and some examples of fluorophores that can be detected in each.

Table 2. Fluorescence channels on the iBright FL1000 Imaging System.

Excitation channel Filter range (nm) Emission channel Filter range (nm) Examples of compatible fluorophores
EX1 455–485 EM1 510–555 Alexa Fluor Plus 488, Alexa Fluor 488, DyLight 488
EX2 515–545 EM2 565–615 Alexa Fluor Plus 555, Alexa Fluor 546, DyLight 550
EX3 610–635 EM3 675–720 Alexa Fluor Plus 647, Alexa Fluor 594, DyLight 650
EX4 655–680 EM4 725–750 Alexa Fluor Plus 680, Alexa Fluor 680, DyLight 680
EX5 745–765 EM5 810–850 Alexa Fluor Plus 800, Alexa Fluor 790, DyLight 800

Table 3. Examples of reagents that can be detected using the iBright FL1000 and CL1000 Imaging Systems.

  FL1000 CL1000
Chemiluminescent detection reagents
SuperSignal West Pico Plus    
SuperSignal West Dura    
SuperSignal West Femto    
Pierce ECL Plus    
Pierce ECL     
WesternBreeze    
Colorimetric detection reagents
1-Step Ultra TMB Blotting Solution    
1-Step NBT/BCIP    
Protein gel stains
Coomassie stains    
Silver stains    
SYPRO Ruby    
SYPRO Orange    
SYPRO Tangerine    
SYPRO Red    
Coomassie Fluor Orange    
Nucleic acid dyes/stains
Ethidium bromide    
SYBR Green I    
SYBR Green II    
SYBR Safe    
SYBR Gold    
Visible fluorophores
Alexa Fluor 488, Alexa Fluor Plus 488    
Alexa Fluor 546    
Alexa Fluor 555, Alexa Fluor Plus 555    
Alexa Fluor 568    
Alexa Fluor 594    
Alexa Fluor 633    
DyLight 488    
DyLight 550    
DyLight 633    
WesternDot 585    
WesternDot 625    
NIR fluorophores
Alexa Fluor 647, Alexa Fluor Plus 647    
Alexa Fluor 660    
Alexa Fluor 680, Alexa Fluor Plus 680    
Alexa Fluor 790    
Alexa Fluor Plus 800    
DyLight 650    
DyLight 680    
DyLight 800    
WesternDot 655    
WesternDot 800