wl,ex,wl,em,Data copyright Molecular Probes 
300,21.22061966,400,0.034380823
301,20.48435068,401,0.039999268
302,19.60805602,402,0.046532821
303,19.28339859,403,0.049233903
304,18.86464035,404,0.051987424
305,18.61534868,405,0.055576071
306,18.29597753,406,0.060085778
307,18.08639989,407,0.066218696
308,17.94039101,408,0.06866071
309,17.98200254,409,0.07559997
310,18.08582621,410,0.080381988
311,18.293566,411,0.090822525
312,18.60472013,412,0.106325324
313,18.95348079,413,0.117846425
314,19.49430199,414,0.133533866
315,19.97485127,415,0.16069189
316,20.80684713,416,0.181888039
317,21.65301893,417,0.218107443
318,22.62679661,418,0.24817256
319,23.4814334,419,0.287677483
320,24.8081006,420,0.330179271
321,25.88043935,421,0.374717086
322,27.38774786,422,0.423426777
323,28.94036799,423,0.461739107
324,30.4493391,424,0.489562601
325,32.34275696,425,0.532837193
326,34.23035862,426,0.575199264
327,36.11588776,427,0.620323778
328,37.97121008,428,0.678795267
329,40.02091289,429,0.736295052
330,42.14146412,430,0.820784479
331,44.41476733,431,0.935240077
332,46.48722597,432,1.041010251
333,48.78945223,433,1.163214659
334,51.14038816,434,1.292796324
335,53.42388689,435,1.479920778
336,55.63052931,436,1.64256154
337,57.86352181,437,1.853880474
338,60.06933718,438,2.084145496
339,62.2838322,439,2.339407374
340,64.46846996,440,2.648622552
341,66.90770062,441,2.973435314
342,69.0148375,442,3.329249868
343,71.27843203,443,3.729791427
344,73.38446214,444,4.177194446
345,75.23883403,445,4.648042538
346,77.44893881,446,5.229081837
347,79.7556387,447,5.930403046
348,81.61652092,448,6.457381568
349,83.74083996,449,6.763803265
350,85.37070165,450,7.450949081
351,87.35042735,451,8.184288544
352,88.62085926,452,8.860770384
353,90.41948289,453,9.760088608
354,91.79802145,454,10.64201755
355,93.02292315,455,11.59263952
356,94.19575222,456,12.53766133
357,95.14621388,457,13.46238297
358,95.98618781,458,14.48514187
359,96.83518114,459,15.70819134
360,97.39035135,460,16.97443418
361,98.15159356,461,18.06405378
362,98.28346389,462,19.05518972
363,98.60068576,463,20.29344838
364,99.18239801,464,21.84995304
365,99.48145764,465,23.54155652
366,99.64823293,466,24.50099325
367,100,467,25.33643021
368,99.91654436,468,26.92030807
369,99.88367977,469,28.64082893
370,99.73195772,470,30.51558846
371,99.2089317,471,32.67989023
372,98.93831824,472,33.36561571
373,98.12776367,473,34.40262191
374,97.48229341,474,37.07299051
375,96.38562944,475,38.85903258
376,95.52368535,476,40.58131115
377,94.21939008,477,42.56265635
378,93.39417645,478,44.66442386
379,91.99794467,479,46.43321248
380,90.40160817,480,47.60283905
381,88.45612896,481,49.03958707
382,86.86330561,482,50.83840491
383,85.16664138,483,52.23989051
384,83.81138635,484,53.78912523
385,82.01058201,485,55.88532717
386,80.19748549,486,57.59788464
387,78.71164475,487,59.40316246
388,77.05570292,488,61.55581078
389,75.16187516,489,63.58235037
390,73.7403622,490,64.91607294
391,71.94233779,491,65.10650002
392,70.29648859,492,66.69032288
393,68.60541565,493,68.6119579
394,66.90910214,494,70.38813768
395,65.27797238,495,72.05997323
396,63.45173685,496,74.10808021
397,61.76438062,497,75.57938442
398,59.60664697,498,77.31448001
399,57.75826917,499,79.02725638
400,55.54430949,500,80.59062978
401,53.35039516,501,81.65105119
402,51.16825258,502,82.91540337
403,48.89614578,503,84.12167777
404,46.67795517,504,85.21216555
405,44.56757931,505,86.31446501
406,42.69644853,506,87.47807096
407,40.4478151,507,88.77333008
408,38.08780691,508,89.75468326
409,36.07603011,509,90.89292691
410,33.89385808,510,91.6514673
411,32.09013209,511,92.7681843
412,30.16818307,512,93.23430381
413,28.34875562,513,93.91490571
414,26.52074976,514,94.84326295
415,24.73261408,515,95.5594125
416,23.18917998,516,96.2661632
417,21.52878568,517,96.87300288
418,20.07168459,518,97.8010528
419,18.63065202,519,98.90355301
420,17.29690445,520,99.00535981
421,15.99845973,521,99.24608992
422,14.71258147,522,99.19029669
423,13.53998566,523,99.39806967
424,12.5328874,524,99.41194411
425,11.40733846,525,99.58126447
426,10.5408206,526,100
427,9.649353496,527,99.93620359
428,8.910819191,528,99.44767226
429,8.100167475,529,98.68103867
430,7.359325999,530,98.13380151
431,6.715811966,531,97.59592434
432,6.141788133,532,97.05198854
433,5.562966257,533,96.75723854
434,5.064348168,534,96.61266177
435,4.574531867,535,96.04303598
436,4.139105159,536,95.22762821
437,3.705930563,537,94.43729908
438,3.344377221,538,93.63363234
439,2.993844241,539,92.88527909
440,2.688737939,540,91.60154338
441,2.393162393,541,90.6107872
442,2.126835718,542,89.53306202
443,1.872950599,543,88.87934468
444,1.612437601,544,88.04739649
445,1.435710616,545,86.48303778
446,1.262593057,546,84.5160031
447,1.149810802,547,83.14465333
448,1.019446586,548,81.63237581
449,0.937935569,549,80.66202564
450,0.84957265,550,79.75538197
451,0.77035104,551,78.49271991
452,0.705723585,552,76.91546022
453,0.646172441,553,75.37331964
454,0.577772448,554,74.00930174
455,0.516217999,555,72.84595444
456,0.473218929,556,71.48877526
457,0.432825855,557,70.15418696
458,0.407869951,558,68.83182936
459,0.367454068,559,67.75526879
460,0.331466203,560,66.43466487
461,0.308524677,561,65.18932993
462,0.278964296,562,63.82508056
463,0.269995358,563,62.76712438
464,0.268225641,564,61.517167
465,0.258413675,565,60.43484052
466,0.252201761,566,59.44154514
467,0.25724093,567,58.25300805
468,0.266725849,568,56.93474359
469,0.26204838,569,55.85655803
470,0.264063052,570,54.52446918
,,571,53.47160004
,,572,52.48221012
,,573,51.33502615
,,574,50.32943594
,,575,49.33993482
,,576,48.24339263
,,577,47.00091363
,,578,46.03031868
,,579,44.95952419
,,580,44.07055097
,,581,43.22057346
,,582,42.44332486
,,583,41.40528314
,,584,40.23218045
,,585,39.32118335
,,586,38.52277974
,,587,37.6357539
,,588,36.57617116
,,589,35.80742616
,,590,34.99134866
,,591,34.1861104
,,592,33.14620038
,,593,32.29417526
,,594,31.38712907
,,595,30.85132907
,,596,30.23844452
,,597,29.57141508
,,598,28.77540809
,,599,28.11039416
,,600,27.42574955
,,601,26.77258092
,,602,25.8963796
,,603,25.14478308
,,604,24.62169946
,,605,24.14177451
,,606,23.66504688
,,607,23.10644066
,,608,22.46631385
,,609,21.71755919
,,610,21.1165572
,,611,20.53340324
,,612,20.04323352
,,613,19.5877442
,,614,19.17699292
,,615,18.70319828
,,616,18.20556126
,,617,17.70031323
,,618,16.95464804
,,619,16.62696823
,,620,16.19057573
,,621,15.64174816
,,622,15.24135881
,,623,14.83757208
,,624,14.46395323
,,625,14.06881306
,,626,13.70636258
,,627,13.45077776
,,628,13.14295486
,,629,12.89288796
,,630,12.45850236
,,631,12.10772006
,,632,11.71702593
,,633,11.39505111
,,634,10.97879857
,,635,10.68506429
,,636,10.56661309
,,637,10.37331266
,,638,9.995118017
,,639,9.75908984
,,640,9.63949283
,,641,9.255773776
,,642,8.963417194
,,643,8.821827401
,,644,8.500754603
,,645,8.28571579
,,646,8.088176474
,,647,7.81740548
,,648,7.617499538
,,649,7.484033174
,,650,7.174478636
,,651,6.980691861
,,652,6.918570761
,,653,6.746082939
,,654,6.523379372
,,655,6.466191654
,,656,6.258752163
,,657,6.012741374
,,658,5.911652179
,,659,5.677046001
,,660,5.653428245
,,661,5.542421241
,,662,5.292336646
,,663,5.172423372
,,664,5.129817576
,,665,4.863013865
,,666,4.716428261
,,667,4.500188049
,,668,4.399824318
,,669,4.239556375
,,670,4.197438245
,,671,4.080477029
,,672,3.96785498
,,673,3.875865954
,,674,3.753702472
,,675,3.6488731
,,676,3.549910207
,,677,3.433046447
,,678,3.310308915
,,679,3.300106585
,,680,3.246917259
,,681,3.157519804
,,682,3.062568986
,,683,3.029025616
,,684,2.968493971
,,685,2.951762695
,,686,2.820688563
,,687,2.746792774
,,688,2.66365585
,,689,2.65790983
,,690,2.567337071
,,691,2.439216021
,,692,2.392564043
,,693,2.428650666
,,694,2.313124211
,,695,2.277848711
,,696,2.190314665
,,697,2.108000263
,,698,2.12594147
,,699,2.06119618
,,700,1.999371801