HRP (horseradish peroxidase) is an enzyme frequently used as an indicator in biochemical assays. It is used to amplify signals and enhance a target’s detectability. HRP acts on various chromogenic substrates to produce many different colors and acts on chemiluminescent substrates to produce light.

HRP is often conjugated to an antibody to detect a target molecule: the antibody specifically locates the target, and HRP, in company with a substrate, produces a detectable signal. HRP is also regularly used in double labeling techniques when researchers want to simultaneously visualize different targets in the same sample. Invitrogenanti-HRP antibodies are designed to reliably detect HRP, and each antibody is validated for use in western blot, immunocytochemistry, and ELISA.

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Western blot analysis of HRP using an anti-HRP monoclonal antibody (Cat. No. MA5-15367) that recognizes full-length HRP recombinant protein.