The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. The TAP tag historically consists of a calmodulin binding peptide (CPB), a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP method including the combination of FLAG tags and HA tags.

The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. The TAP tag has been especially useful and deployed with Yeast Tap-tagged ORF clones. These clones contain genomic fusions of the TAP construct and are extremely useful for determining natural protein interactions and expression level variations based on physiological changes.

Invitrogen TAP tag antibodies dependably detect TAP tagged proteins, and each antibody is validated for use in a variety of applications.

See all anti-TAP tag antibodies

Annotated product references

Cat. No. CAB1001 was used in western blot to examine Set1/COMPASS-mediated histone H3 Lys4 trimethylation. Genes & development (Jan 2014; 28: 115) "Context dependency of Set1/COMPASS-mediated histone H3 Lys4 trimethylation." Thornton JL,Westfield GH,Takahashi YH,Cook M,Gao X,Woodfin AR,Lee JS,Morgan MA,Jackson J,Smith ER,Couture JF,Skiniotis G,Shilatifard A

Cat. No. CAB1001 was used in immunoprecipitation and western blot to study the functions of GPN small GTPases in Saccharomyces cerevisiae. Genetics (Mar 2013; 193: 853) "Biogenesis of RNA polymerases II and III requires the conserved GPN small GTPases in Saccharomyces cerevisiae." Minaker SW,Filiatrault MC,Ben-Aroya S,Hieter P,Stirling PC

Cat. No. CAB1001 was used in western blot to develop a systematic approach for studying protein complexes in living cells. Cell reports (Jun 2013; 3: 2155) "A systematic approach for the genetic dissection of protein complexes in living cells." Diss G,Dubé AK,Boutin J,Gagnon-Arsenault I,Landry CR