Your choice for optimal scale-up

Start your scale-up with a T225 flask that has the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes an angled neck and wide opening, allowing easy access to the growth surface by pipettes or cell scrapers.

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What makes our Nunc EasYFlasks so easy to use?

Angled neck


  • Ergonomic design for easy access to the growth surface by pipettes or cell scrapers
  • Low profile to maximize incubator space
  • Slightly angled side walls for complete viewing under a microscope
  • Volume graduations printed and molded on both sides
  • Sterilized by gamma irradiation and certified to be non-pyrogenic

Filter or vent/close cap

Filter caps

  • Effective barrier against micro-organisms
  • Continuous venting with consistent gas exchange

Vent/close Caps

  • “Y” mark allows visual verification of vent position: open or closed

Understand your T225 cell culture flask surface for adherent cells

To help ensure flexible, reproducible and reliable results across every stage of cell culture, we offer a range of cell culture flasks spanning a variety of formats, sizes and surfaces. We can help guide your product selection with specific cell types and desired cell culture ranges in mind.

For additional guidance on usage by cell type, view our Surface Selection Guide .

Surface technology designed for adherent cell cultures

The Thermo Scientific Nunclon Delta cell culture surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. The Nunclon Delta certificate ensures high quality standards and lot-to-lot consistency. Look for it on a variety of Thermo Scientific cell culture plastics. 

T225 flasks for adherent cells

The proprietary Nunclon Delta cell culture surface supports optimal growth and attachment of cells to the growth surface.

  Neck Surface Material Cap Cap feature
EasYFlask Angled Nunclon Delta Cell culture-treated polystyrene Filter constant airflow
EasYFlask Angled Nunclon Delta Cell culture-treated polystyrene Vent/close sufficient gas exchange

Related flask and cell culture resources

Surface selection: Achieving optimal cell growth and harvesting

Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.

  • Surface modifications to improve cell adhesion
  • Characterization of Nunc cell culture surfaces by adhesion properties
  • Alternative methods for enzyme-free cell harvesting

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Featured article

Cell adhesion and growth on coated or modified glass or plastic surfaces

Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type–specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge. 

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