The One Shot FBS 50 mL bottle

The One Shot FBS 50 mL bottle

An aliquot-free solution to minimize contamination and save time.

The Gibco One Shot FBS 50 mL bottle is designed to significantly improve your results and solve myriad challenges slowing down your research. 

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Take control of your results by putting an end to aliquoting.

FBS workflow

Researcher quote

“This bottle allows for convenience of speed while maintaining a sterile product. As we have lots of undergrads in and out of the lab, this ensures that one mishap with not-so-aged hands doesn't ruin a whole pack of FBS.” Bethany C. Prudner, PhD, Metabolomic Sarcoma Lab, Washington University in St Louis

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End aliquoting

By aliquoting your FBS into 50 mL conical tubes, you increase the chances of compromising your results. The One Shot FBS 50 mL bottle’s size and design eliminates that step, putting you in control of your time, contamination risk, and volume consistency.

Pour and pipet with ease

The bottle’s wide mouth and chamfered edge enable smooth, burp-free pouring. And, unlike with the current One Shot bottle, you can use a 25 and/or 50 mL pipette. Whichever way you go, you’ll get to every last drop. 

Stop the freeze-thaw-repeat cycle

Skipping the aliquot step means you only have to freeze then thaw your FBS once—saving considerable time and effort. And the bottles are engineered to withstand freezing, so they won’t crack and pop their lids like conical tubes often do.

End labeling mishaps

Missing SKU or lot numbers, expiration dates, origin—or even the entire label—can cause a chain reaction of problems. The One Shot bottle has an easy-to-read package label, so you know exactly what you’re using. 

Free up freezer space

The One Shot FBS package design is optimized to ease the burden on your freezer capacity.

In fact, the One Shot 10 x 50 mL carton allows you to store up to 35 L more FBS in an average 20 ft. freezer than our current 40 x 50 mL One Shot carton, and 57 L more than our 100 mL bottle.

Pack the rack

Your test tube racks aren’t just for conical tubes anymore. The One Shot FBS 50 mL bottle fits perfectly in most test tube racks—including strong, durable 30 mm Nalgene Unwire Racks. So you get the convenience of fit in an easy-pour, easy-store, aliquot-free bottle.

Create less waste

The One Shot FBS 50 mL bottle generates 33% less waste than aliquoting from 500 mL bottles (see table below). By using less plastic, less petroleum feedstock is consumed, and fewer greenhouse gas emissions are generated. In the lab, a typical customer using 20 L of FBS per year would get back almost 7 hours and generate 7 lb less plastic waste by choosing One Shot FBS. This is a significant benefit both to lab productivity and the environment.

Method Step in procedure Plastic required Weight (g) Total plastic weight (g)
Aliquot from 500 mL bottle Original container 1 x 500 mL bottle 79.4  
Aliquot to conicals 1 x 25 mL serological pipette 18.8  
10 conical tubes 140.3 238.5
One Shot FBS 50 mL Bottles Original container 10 x 50 mL bottles 159.0 159.0
  33% waste reduction


Pair for best performance

The 50 mL bottle size is a perfect match for use with a 500 mL bottle of Gibco RPMI, DMEM, or other Gibco media. The complete media pairing will help maximize performance and reproducibility.

For questions about the One Shot FBS 50 mL bottle's specifications, contact technical services at

Available in single 50 mL bottle or 10-bottle pack

The One Shot carton configuration is 10 x 50 mL.

Our Specialty FBS, qualified for specialty research and specific assays is now available in the Gibco One Shot FBS 50 mL bottle.

Learn more about our specialty FBS products at

For Research Use or Further Manufacturing Use only. Serum and blood proteins are not for direct administration into humans or animals.