Rat Cortex and Hippocampus Neuronal Cells   Gibco Primary Rat Cortex and Hippocampus Neurons are isolated from day-18 Fisher 344 rat embryos and cryopreserved in liquid nitrogen. These cells offer neuroscience researchers a flexible, ready-to-use, high-quality alternative to freshly isolated neurons.

Rat cortex and hippocampus neuronal cells

These products are developed through vigorous internal research to afford comparable properties, such as viability, purity, and cell function, but minimize the inherent lot-to-lot variability with cells that are acutely isolated. Unlike the other commercially available primary rat neurons that generally yield about 30% or lower post-thaw viability, Gibco neuron cells consistently give post-thaw viability in the range of 50–80% and cells grown in Neurobasal medium plus B-27 supplements show minimum or absence of glial cell growth.

Graph showing post-thaw viability for rat cortical neurons


Figure 1. Comparison of post thaw viability of primary rat cortex neurons with competitors' products.

Graph showing post-thaw viability of rat hippocampus neurons


Figure 2. Comparison of post thaw viability of primary hippocampus neurons with competitors' products.

Graph showing post-thaw viability for rat cortical neurons


Figure 1. Comparison of post thaw viability of primary rat cortex neurons with competitors' products.

Graph showing post-thaw viability of rat hippocampus neurons


Figure 2. Comparison of post thaw viability of primary hippocampus neurons with competitors' products.

Primary rat cortical neurons were maintained for 3 weeks in Gibco B-27 Plus Neuronal Culture System  containing B-27 Plus Supplement and Neurobasal Plus Medium.

Figure 3. Primary rat cortical neurons were maintained for 3 weeks in Gibco B-27 Plus Neuronal Culture System  containing B-27 Plus Supplement and Neurobasal Plus Medium. Neuronal survival was measured by immunofluorescent labeling of MAP2 (green), performed on days 7, 14 and 21.

Long-term viability of neurons using the LIVE/DEAD Viability/Cytotoxicity assay

Rat cortical neurons cultured in Neurobasal medium supplemented with B-27 supplement and GlutaMAX I day seven
Rat cortical neurons cultured in Neurobasal medium supplemented with B-27 supplement and GlutaMAX I day fourteen
Rat cortical neurons cultured in Neurobasal medium supplemented with B-27 supplement and GlutaMAX I day twentyone

Figure 4. Long-term viability of neurons using the LIVE/DEAD Viability/Cytotoxicity assay. Rat cortical neurons cultured in Neurobasal medium supplemented with B-27 supplement and GlutaMAX-I. Live cells are stained with calcein-AM (green) and dead cells are stained with EthD-1 (red); (LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells).
 

Long-term viability of neurons using the B27 Plus Neuronal Culture System

Cryopreserved Rat Cortical Neurons (A1084002) was thawed in classic Neurobasal B-27 medium and then plated onto poly-D-lysine coated 96-well plates into B-27 Plus Neuronal Culture System consisting of Neurobasal Plus medium and B-27 Plus Supplement.
Rat Cortical Neurons (4 Weeks)
Cryopreserved Rat Hippocampal Neurons (A1084101) was thawed in classic Neurobasal B-27 medium and then plated onto poly-D-lysine coated 96-well plates into B-27 Plus Neuronal Culture System consisting of Neurobasal Plus medium and B-27 Plus Supplement.

Rat Hippocampal Neurons (4 Weeks)

Figure 5. Cryopreserved Primary Rat Cortical Neurons and Primary Rat Hippocampal Neurons were thawed in classic Neurobasal and B-27 medium and then plated onto poly-D-lysine coated 96-well plates into Gibco B-27 Plus Neuronal Culture System. Neurons were cultured 4 weeks in Neurobasal Plus medium supplemented with B-27 Plus and GlutaMAX were stained with neuronal dendritic marker, MAP2 (green) and neuronal cell body marker, HuC/D (red), and nuclei were counter-stained with DAPI (blue). Immunostaining was performed at indicated time points.