Single Letter Code

B = C or G or T
D = A or G or T
H = A or C or T
K = G or T
M = A or C
N = A or C or G or T

R = A or G
S = C or G
V = A or C or G
W = A or T
Y = C or T

Catalog #ProductIso-
schizomers
Sequence & Cut SiteOverhangAnza Restriction Enzymes Resulting in Compatible EndsOptimal Reaction Tempe-
rature
Thermal Inactiva-
tion
Anza Alkaline Phos ProtocolMethylation Effects
IVGN0014Anza 1 NotICciNIGCGGCCGC

5'...G C^G G C C G C...3'
3'...C G C C G G^C G...5'
5’ protruding endsAnza 47 Eco52I, Anza 121 Bsp120I
IVGN0026Anza 2 NcoIBsp19ICCATGG

5'...C^C A T G G...3'
3'...G G T A C^C...5'
5’ protruding endsAnza 58 PagI
IVGN0034Anza 3 BcuIAhlI, SpeIACTAGT

5'...A^C T A G T...3'
3'...T G A T C^A...5'
5’ protruding endsAnza 6 NheI, Anza 12 XbaI, Anza 15 XmaJI
IVGN0044Anza 4 BpiIBbsI, BstV2IGAAGAC(2/6)

5'...G A A G A C (N)2^...3'
3'...C T T C T G (N)6^...5'
5’ protruding ends
IVGN0056Anza 5 BamHIGGATCC

5'...G^G A T C C...3'
3'...C C T A G^G...5'
5’ protruding endsAnza 19 BglII, Anza 55 MboI, Anza 73 BclI, Anza 97 Bsp143I
IVGN0066Anza 6 NheIAsuNHIGCTAGC

5'...G^C T A G C...3'
3'...C G A T C^G...5'
5’ protruding endsAnza 3 BcuI, Anza 12 XbaI, Anza 15 XmaJI
IVGN0074Anza 7 BshTIAsiGI, CspAI, PinAI, AgeIACCGGT

5'...A^C C G G T...3'
3'...T G G C C^A...5'
5’ protruding endsAnza 60 Kpn2I, Anza 85 MreI, Anza 90 Eco88I* (*if recognized sequence is 5'-CCCGGG-3')
IVGN0086Anza 8 XhoIPaeR7I, Sfr274I, SlaICTCGAG

5'...C^T C G A G...3'
3'...G A G C T^C...5'
5’ protruding endsAnza 14 SalI, Anza 90 Eco88I* (*if recognized sequnece is 5'-CTCGAG-3')
IVGN0096Anza 9 NdeIFauNDICATATG

5'...C A^T A T G...3'
3'...G T A T^A C...5'
5’ protruding endsAnza 64 SaqAI, Anza 76 VspI, Anza 80 FspBI, Anza 122 Csp6I
IVGN0106Anza 10 DpnIMalIGm6ATC

5'...G m6A^T C...3'
3'...C T^m6A G...5'
blunt
IVGN0116Anza 11 EcoRIGAATTC

5'...G^A A T T C...3'
3'...C T T A A^G...5'
5’ protruding endsAnza 31 MunI, Anza 111 XapI
IVGN0126Anza 12 XbaITCTAGA

5'...T^C T A G A...3'
3'...A G A T C^T...5'
5’ protruding endsAnza 3 BcuI, Anza 6 NheI, Anza 15 XmaJI
IVGN0136Anza 13 Esp3IBsmBI CGTCTC(1/5)

5'...C G T C T C (N)1^...3'
3'...G C A G A G (N)5^...5'
5’ protruding ends
IVGN0146Anza 14 SalIGTCGAC

5'...G^T C G A C...3'
3'...C A G C T^G...5'
5’ protruding endsAnza 8 XhoI, Anza 90 Eco88I* (*if recognized sequnece is 5'-CTCGAG-3')
IVGN0154Anza15 XmaJIAspA2I, AvrII, BlnI CCTAGG

5'...C^C T A G G...3'
3'...G G A T C^C...5'
5’ protruding endsAnza 3 BcuI, Anza 6 NheI, Anza 12 XbaI
IVGN0166Anza 16 HindIIIAAGCTT

5'...A^A G C T T...3'
3'...T T C G A^A...5'
5’ protruding ends
IVGN0176Anza 17 KpnIGGTACC

5'...G G T A C^C...3'
3'...C^C A T G G...5'
3’ protruding ends
IVGN0184Anza 18 PacITTAATTAA

5'…T T A A T^T A A...3'
3'…A A T^T A A T T...5'
3’ protruding endsAnza 27 PvuI, Anza 40 SfaAI
IVGN0196Anza 19 BglIIAGATCT

5'...A^G A T C T...3'
3'...T C T A G^A...5'
5’ protruding endsAnza 5 BamHI, Anza 55 MboI, Anza 73 BclI, Anza 97 Bsp143I
IVGN0206Anza 20 SacI Psp124BI, SstIGAGCTC

5'...G A G C T^C...3'
3'...C^T C G A G...5'
3’ protruding endsAnza 126 Alw21I* (*if recognized sequence is 5'-GAGCTC-3')
IVGN0214Anza 21 SgsIAscI, PalAI GGCGCGCC

5'...G G^C G C G C C...3'
3'...C C G C G C^G G...5'
5’ protruding endsAnza 28 MluI, Anza 45 PteI, Anza 95 MauBI
IVGN0226Anza 22 SmaICCCGGG

5'...C C C^G G G...3'
3'...G G G^C C C...5'
blunt
IVGN0236Anza 23 PstICTGCAG

5'...C T G C A^G...3'
3'...G^A C G T C...5'
3’ protruding endsAnza 37 Mph1103I, Anza 126 Alw21I* (*if recognized sequence is 5'-GTGCAC-3')
IVGN0244Anza 24 MssIPmeIGTTTAAAC

5'...G T T T^A A A C...3'
3'...C A A A^T T T G...5'
blunt
IVGN0254Anza 25 PaeISphI GCATGC

5'...G C A T G^C...3'
3'...C^G T A C G...5'
3’ protruding endsAnza 56 Hin1II, Anza 98 XceI
IVGN0266Anza 26 Eco32IEcoRV GATATC

5'...G A T^A T C...3'
3'...C T A^T A G...5'
blunt
IVGN0274Anza 27 PvuI Ple19I CGATCG

5'...C G A T^C G...3'
3'...G C^T A G C...5'
3’ protruding endsAnza 18 PacI, Anza 40 SfaAI
IVGN0286Anza 28 MluIACGCGT

5'...A^C G C G T...3'
3'...T G C G C^A...5'
5’ protruding endsAnza 21 SgsI, Anza 45 PteI, Anza 95 MauBI
IVGN0294Anza 29 KflIGGGWCCC

5'…G G^G W C C C...3'
3'…C C C W G^G G...5'
5’ protruding endsAnza 63 CpoI, Anza 87 Eco47I
IVGN0306Anza 30 Bsu15I Bsa29I, BseCI, BshVI, BspDI, BsuTUI, ClaIATCGAT

5'...A T^C G A T...3'
3'...T A G C^T A...5'
5’ protruding endsAnza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3')
IVGN0314Anza 31 MunIMfeI CAATTG

5'...C^A A T T G...3'
3'...G T T A A^C...5'
5’ protruding endsAnza 11 EcoRI, Anza 111 XapI
IVGN0326Anza 32 ApaIGGGCCC

5'...G G G C C^C...3'
3'...C^C C G G G...5'
3’ protruding ends
IVGN0334Anza 33 LguIBspQI, PciSI, SapI GCTCTTC(1/4)

5'...G C T C T T C (N)1^...3'
3'...C G A G A A G (N)4^...5'
5’ protruding ends
IVGN0344Anza 34 Pfl23IIBsiWI, PspLI CGTACG

5'...C^G T A C G...3'
3'...G C A T G^C...5'
5’ protruding endsAnza 39 Bsp1407I, Anza 91 Acc65I, Anza 129 BshNI* (*if recognized sequence is 5'-GGTACC-3')
IVGN0354Anza 35 Eco47IIIAfeI, Aor51HI AGCGCT

5'...A G C^G C T...3'
3'...T C G^C G A...5'
blunt
IVGN0366Anza 36 Eco31IBsaI, Bso31I, BspTNIGGTCTC(1/5)

5'...G G T C T C (N)1^...3'
3'...C C A G A G (N)5^...5'
5’ protruding ends
IVGN0376Anza 37 Mph1103IEcoT22I, NsiI, Zsp2I ATGCAT

5'...A T G C A^T...3'
3'...T^A C G T A...5'
3’ protruding endsAnza 23 PstI, Anza 126 Alw21I* (*if recognized sequence is 5'-GTGCAC-3')
IVGN0386Anza 38 ScaIBmcAI, ZrmI AGTACT

5'...A G T^A C T...3'
3'...T C A^T G A...5'
blunt
IVGN0396Anza 39 Bsp1407IBsrGI, BstAUI TGTACA

5'...T^G T A C A...3'
3'...A C A T G^T...5'
5’ protruding endsAnza 34 Pfl23II, Anza 91 Acc65I, Anza 129 BshNI* (*if recognized sequence is 5'-GGTACC-3')
IVGN0404Anza 40 SfaAIAsiSI, RgaI, SgfI GCGATCGC

5'...G C G A T^C G C...3'
3'...C G C^T A G C G...5'
3’ protruding endsAnza 18 PacI, Anza 27 PvuI
IVGN0416Anza 41 HpyF3IBstDEI, DdeI CTNAG

5'...C^T N A G...3'
3'...G A N T^C...5'
5’ protruding ends
IVGN0426Anza 42 RsaIAfaIGTAC

5'...G T^A C...3'
3'...C A^T G...5'
blunt
IVGN0434Anza 43 Eco105IBstSNI, SnaBI TACGTA

5'...T A C^G T A...3'
3'...A T G^C A T...5'
blunt
IVGN0446Anza 44 AluIAluBI AGCT

5'...A G^C T...3'
3'...T C^G A...5'
blunt
IVGN0454Anza 45 PteIBsePI, BssHII, PauIGCGCGC

5'...G^C G C G C...3'
3'...C G C G C^G...5'
5’ protruding endsAnza 21 SgsI, Anza 28 MluI, Anza 95 MauBI
IVGN0464Anza 46 AatIIGACGTC

5'...G A C G T^C...3'
3'...C^T G C A G...5'
3’ protruding ends
IVGN0474Anza 47 Eco52IBseX3I, BstZI, EagI, EclXI CGGCCG

5'...C^G G C C G...3'
3'...G C C G G^C...5'
5’ protruding endsAnza 1 NotI, Anza 121 Bsp120I
IVGN0484Anza 48 MnlICCTC(7/6)

5'...C C T C (N)7^...3'
3'...G G A G (N)6^...5'
3’ protruding ends
IVGN0496Anza 49 SmiISwaI ATTTAAAT

5'...A T T T^A A A T...3'
3'...T A A A^T T T A...5'
blunt
IVGN0504Anza 50 KspAIHpaI GTTAAC

5'...G T T^A A C...3'
3'...C A A^T T G...5'
blunt
IVGN0516Anza 51 BspTIAflII, BfrI, BstAFI, MspCI, Vha464I CTTAAG

5'...C^T T A A G...3'
3'...G A A T T^C...5'
5’ protruding ends
IVGN0526Anza 52 PvuIICAGCTG

5'...C A G^C T G...3'
3'...G T C^G A C...5'
blunt
IVGN0534Anza 53 AanIPsiI TTATAA

5'...T T A^T A A...3'
3'...A A T^A T T...5'
blunt
IVGN0546Anza 54 Eco147IPceI, SseBI, StuI AGGCCT

5'...A G G^C C T...3'
3'...T C C^G G A...5'
blunt
IVGN0554Anza 55 MboIBfuCI, Bsp143I, BssMI, BstMBI, DpnII, Kzo9I, NdeII, Sau3AIGATC

5'...^G A T C ...3'
3'... C T A G^...5'
5’ protruding endsAnza 5 BamHI, Anza 19 BglII, Anza 73 BclI, Anza 97 Bsp143I
IVGN0566Anza 56 Hin1IIFaeI, Hsp92II, NlaIII CATG

5'... C A T G^...3'
3'...^G T A C ...5'
3’ protruding endsAnza 25 PaeI, Anza 98 XceI
IVGN0574Anza 57 Bpu1102IBlpI, Bsp1720I GCTNAGC

5'...G C^T N A G C...3'
3'...C G A N T^C G...5'
5’ protruding ends
IVGN0584Anza 58 PagICciI, BspHI TCATGA

5'...T^C A T G A...3'
3'...A G T A C^T...5'
5’ protruding endsAnza 2 NcoI
IVGN0596Anza 59 HhaIAspLEI, BstHHI, CfoI GCGC

5'...G C G^C...3'
3'...C^G C G...5'
3’ protruding ends
IVGN0606Anza 60 Kpn2IAccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI TCCGGA

5'...T^C C G G A...3'
3'...A G G C C^T...5'
5’ protruding endsAnza 7 BshTI, Anza 85 MreI, Anza 90 Eco88I* (*If recognized sequence is 5'-CCCGGG-3')
IVGN0614Anza 61 PfoITCCNGGA

5'...T^C C N G G A...3'
3'...A G G N C C^T...5'
5’ protruding ends
IVGN0624Anza 62 MlsIBalI, MluNI, MscI, Msp20I, BspTNITGGCCA

5'...T G G^C C A...3'
3'...A C C^G G T...5'
blunt
IVGN0634Anza 63 CpoICspI, Rsr2I, RsrII CGGWCCG

5'...C G^G W C C G...3'
3'...G C C W G^G C...5'
5’ protruding endsAnza 28 KflI, Anza 87 Eco47I
IVGN0644Anza 64 SaqAIMseI, Tru1I, Tru9ITTAA

5'...T^T A A...3'
3'...A A T^T...5'
5’ protruding endsAnza 9 NdeI, Anza 76 VspI, Anza 80 FspBI, Anza 122 Csp6I
IVGN0656Anza 65 MspIBsiSI, HapII, Hpall CCGG

5'...C^C G G...3'
3'...G G C^C...5'
5’ protruding endsAnza 30 Bsu15I, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3')
IVGN0666Anza 66 BstXICCANNNNNNTGG

5'...C C A N N N N N^N T G G...3'
3'...G G T N^N N N N N A C C...5'
3’ protruding ends
IVGN0676Anza 67 RruIBsp68I, BtuMI, NruITCGCGA

5'...T C G^C G A...3'
3'...A G C^G C T...5'
blunt
IVGN0686Anza 68 BsuRIBshFI, BsnI, HaeIII, BspANIGGCC

5'...G G^C C...3'
3'...C C^G G...5'
blunt
IVGN0696Anza 69 BglIGCCNNNNNGGC

5'...G C C N N N N^N G G C...3'
3'...C G G N^N N N N C C G...5'
3’ protruding ends
IVGN0704Anza 70 NsbIAcc16I, FspITGCGCA

5'...T G C^G C A...3'
3'...A C G^C G T...5'
blunt
IVGN0716Anza 71 HinfIGANTC

5'...G^A N T C...3'
3'...C T N A^G...5'
5’ protruding ends
IVGN0726Anza 72 HincIIHindII GTYRAC

5'...G T Y^R A C...3'
3'...C A R^Y T G...5'
blunt
IVGN0736Anza 73 BclIFbaI, Ksp22I TGATCA

5'...T^G A T C A...3'
3'...A C T A G^T...5'
5’ protruding endsAnza 5 BamHI, Anza 19 BglII, Anza 55 MboI, Anza 97 Bsp143I
IVGN0744Anza 74 CsiIACCWGGT

5'…A^C C W G G T...3'
3'…T G G W C C^A...5'
5’ protruding ends
IVGN0756Anza 75 Alw44IApaLI, VneI GTGCAC

5'...G^T G C A C...3'
3'...C A C G T^G...5'
5’ protruding endsAnza 94 BfmI* (* if recognized sequence is 5'-CTGCAG-3')
IVGN0766Anza 76 VspIAseI, PshBI ATTAAT

5'...A T^T A A T...3'
3'...T A A T^T A...5'
5’ protruding endsAnza 9 NdeI, Anza 64 SaqAI, Anza 80 FspBI, Anza 122 Csp6I
IVGN0776Anza 77 DraITTTAAA

5'...T T T^A A A...3'
3'...A A A^T T T...5'
blunt
IVGN0786Anza 78 AdeIDraIII CACNNNGTG

5'...C A C N N N^G T G...3'
3'...G T G^N N N C A C...5'
3’ protruding ends
IVGN0794Anza 79 PdiINaeIGCCGGC

5'...G C C^G G C...3'
3'...C G G^C C G...5'
blunt
IVGN0804Anza 80 FspBIBfaI, MaeI, XspI, SspMl CTAG

5'...C^T A G...3'
3'...G A T^C...5'
5’ protruding endsAnza 9 NdeI, Anza 64 SaqAI, Anza 76 VspI, Anza 122 Csp6I
IVGN0816Anza 81 Eco91IBstEII, BstPI, EcoO65I, PspEI GGTNACC

5'...G^G T N A C C...3'
3'...C C A N T G^G...5'
5’ protruding ends
IVGN0826Anza 82 Eco72IAcvI, BbrPI, PmaCI, Pm1I, PspCI CACGTG

5'...C A C^G T G...3'
3'...G T G^C A C...5'
blunt
IVGN0836Anza 83 Eco81IAxyI, Bse21I, Bsu36I CCTNAGG

5'...C C^T N A G G...3'
3'...G G A N T^C C...5'
5’ protruding ends
IVGN0844Anza 84 FspAIRTGCGCAY

5'...R T G C^G C A Y...3'
3'...Y A C G^C G T R...5'
blunt
IVGN0854Anza 85 MreICGCCGGCG

5'...C G^C C G G C G...3'
3'...G C G G C C^G C...5'
5’ protruding endsAnza 7 BshTI, Anza 60 Kpn2I, Anza 90 Eco88I* (*If recognized sequence is 5'-CCCGGG-3')
IVGN0866Anza 86 PdmIAsp700I, MroXI, XmnI GAANNNNTTC

5'...G A A N N^N N T T C...3'
3'...C T T N N^N N A A G...5'
blunt
IVGN0876Anza 87 Eco47IAvaII, Bme18I, SinI, VpaK11BI GGWCC

5'...G^G W C C...3'
3'...C C W G^G...5'
5’ protruding endsAnza 28, KflI, Anza 63 CpoI
IVGN0886Anza 88 Bsp119IAsuII, Bpu14I, BspT104I, BstBI, NspV, SfuITTCGAA

5'...T T^C G A A...3'
3'...A A G C^T T...5'
5’ protruding endsAnza 30 Bsu15I, Anza 65 MspI, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3')
IVGN0894Anza 89 Mva1269IBsmI, PctI GAATGC(1/-1)

5'...G A A T G C N^...3'
3'...C T T A C^G N ...5'
3’ protruding ends
IVGN0906Anza 90 Eco88IAma87I, AvaI, BsiHKCI, BsoBI, BmeT110lCYCGRG

5'...C^Y C G R G...3'
3'...G R G C Y^C...5'
5’ protruding endsIf recognized sequence is 5'-CTCGAG-3', then generate compatible ends for Anza 8 XhoI, Anza 14 SalI. If recognized sequence is 5'-CCCGGG-3', then generate compatible ends for Anza 7 BshTI, Anza 60 Kpn2I, Anza 85 MreI
IVGN0916Anza 91 Acc65IAsp718IGGTACC

5'...G^G T A C C...3'
3'...C C A T G^G...5'
5’ protruding endsAnza 34 Pfl23II, Anza 39 Bsp1407I, Anza 129 BshNI* (*if recognized sequence is 5'-GGTACC-3')
IVGN0924Anza 92 EheIDinI, EgeI, SfoIGGCGCC

5'...G G C^G C C...3'
3'...C C G^C G G...5'
blunt
IVGN0936Anza 93 HpaIIBsiSI, HapII, MsplCCGG

5'...C^C G G...3'
3'...G G C^C...5'
5’ protruding endsAnza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3')
IVGN0944Anza 94 BfmIBstSFI, SfcI CTRYAG

5'...C^T R Y A G...3'
3'...G A Y R T^C...5'
5’ protruding endsIf recognized sequnce is 5'-CTGCAG-3', then generate compatible ends for Anza 75 Alw44I
IVGN0954Anza 95 MauBICGCGCGCG

5'...C G^C G C G C G...3'
3'...G C G C G C^G C...5'
5’ protruding endsAnza 21 SgsI, Anza 28 MluI, Anza 45 PteI
IVGN0966Anza 96 XmiIAccI, FblI GTMKAC

5'...G T^M K A C...3'
3'...C A K M^T G...5'
5’ protruding ends If recognized sequence is 5'-GTCGAC-3', then generate compatible ends for Anza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 123 Hin6I
IVGN0976Anza 97 Bsp143IBfuCI, BssMI, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AIGATC

5'...^G A T C ...3'
3'... C T A G^...5'
5’ protruding endsAnza 5 BamHI, Anza 19 BglII, Anza 55 MboI, Anza 73 BclI
IVGN0984Anza 98 XceIBstNSI, NspI RCATGY

5'...R C A T G^Y...3'
3'...Y^G T A C R...5'
3’ protruding endsAnza 25 PaeI, Anza 56 Hin1II
IVGN0996Anza 99 XagIBstENI, EcoNI CCTNNNNNAGG

5'...C C T N N^N N N A G G...3'
3'...G G A N N N^N N T C C...5'
5’ protruding ends
IVGN1006Anza 100 Bsh1236IAccII, BspFNI, BstFNI, BstUI, MvnI CGCG

5'...C G^C G...3'
3'...G C^G C...5'
blunt
IVGN1016Anza 101 BoxIBstPAI, PshAI GACNNNNGTC

5'...G A C N N^N N G T C...3'
3'...C T G N N^N N C A G...5'
blunt
IVGN1024Anza 102 CaiIAlwNI, PstNICAGNNNCTG

5'...C A G N N N^C T G...3'
3'...G T C^N N N G A C...5'
3’ protruding ends
IVGN1034Anza 103 Psp1406IAclI AACGTT

5'...A A^C G T T...3'
3'...T T G C^A A...5'
5’ protruding endsAnza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 105 Hin1I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3')
IVGN1044Anza 104 MboIIGAAGA(8/7)

5'...G A A G A (N)8^...3'
3'...C T T C T (N)7^...5'
3’ protruding ends
IVGN1056Anza 105 Hin1IAcyI, BsaHI, BssNI, BstACI, Hsp92I GRCGYC

5'...G R^C G Y C...3'
3'...C Y G C^R G...5'
5’ protruding endsAnza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 123 Hin6I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3')
IVGN1066Anza 106 Van91IAccB7I, PflMICCANNNNNTGG

5'...C C A N N N N^N T G G...3'
3'...G G T N^N N N N A C C...5'
3’ protruding ends
IVGN1074Anza 107 BspLIBmiI, NlaIV, PspN4I GGNNCC

5'...G G N^N C C...3'
3'...C C N^N G G...5'
blunt
IVGN1084Anza 108 SatIFnu4HI, Fsp4HI GCNGC

5'...G C^N G C...3'
3'...C G N^C G...5'
5’ protruding ends
IVGN1096Anza 109 Alw26IBcoDI, BsmAI, BstMAI GTCTC(1/5)

5'...G T C T C (N)1^...3'
3'...C A G A G (N)5^...5'
5’ protruding ends
IVGN1116Anza 111 XapIAcsI, ApoI RAATTY

5'...R^A A T T Y...3'
3'...Y T T A A^R...5'
5’ protruding endsAnza 11 EcoRI, Anza 31 MunI
IVGN1126Anza 112 BseGIBstF5I, BtsCI GGATG(2/0)

5'...G G A T G N N^...3'
3'...C C T A C^N N ...5'
3’ protruding ends
IVGN1136Anza 113 BcnIAsuC2I, BpuMI, NciI CCSGG

5'...C C^S G G...3'
3'...G G S^C C...5'
5’ protruding ends
IVGN1146Anza 114 Hpy8IHpy166II GTNNAC

5'...G T N^N A C...3'
3'...C A N^N T G...5'
blunt
IVGN1156Anza 115 MbiIAccBSI, BsrBI CCGCTC (-3/-3)

5'...C C G^C T C...3'
3'...G G C^G A G...5'
blunt
IVGN1166Anza 116 Cfr13IAspS9I, BmgT120I, PspPI, Sau96I GGNCC

5'...G^G N C C...3'
3'...C C N G^G...5'
5’ protruding ends
IVGN1176Anza 117 EcoO109IRGGNCCY

5'...R G^G N C C Y...3'
3'...Y C C N G^G R...5'
5’ protruding ends
IVGN1186Anza 118 BseDIBsaJI, BssECI CCNNGG

5'...C^C N N G G...3'
3'...G G N N C^C...5'
5’ protruding ends
IVGN1194Anza 119 BmsILweI, SfaNIGCATC (5/9)

5'…G C A T C (N)5^...3'
3'…C G T A G (N)9^...5'
5’ protruding ends
IVGN1204Anza 120 NmuCITseFI, Tsp45I GTSAC

5'...^G T S A C ...3'
3'... C A S T G^...5'
5’ protruding ends
IVGN1216Anza 121 Bsp120IPspOMI GGGCCC

5'...G^G G C C C...3'
3'...C C C G G^G...5'
5’ protruding endsAnza 1 NotI, Anza 47 Eco52I
IVGN1226Anza 122 Csp6ICviQI, RsaNIGTAC

5'...G^T A C...3'
3'...C A T^G...5'
5’ protruding endsAnza 9 NdeI, Anza 64 SaqAI, Anza 76 VspI, Anza 80 FspBI
IVGN1236Anza 123 Hin6IHinP1I, HspAI GCGC

5'...G^C G C...3'
3'...C G C^G...5'
5’ protruding endsAnza 30 Bsu15I, Anza 65 MspI, Anza 88 Bsp119I, Anza 93 HpaII, Anza 103 Psp1406I, Anza 105 Hin1I, Anza 96 XmiI* (*if recognized sequence is 5'-GTCGAC-3')
IVGN1244Anza 124 PfeITfiI GAWTC

5'...G^A W T C...3'
3'...C T W A^G...5'
5’ protruding ends
IVGN1254Anza 125 HpyF10VIBstMWI, MwoIGCNNNNNNNGC

5'...G C N N N N N^N N G C...3'
3'...C G N N^N N N N N C G...5'
3’ protruding ends
IVGN1266Anza 126 Alw21IBbv12I, BsiHKAI GWGCWC

5'...G W G C W^C...3'
3'...C^W C G W G...5'
3’ protruding endsIf recognized sequence is 5'-GAGCTC-3', then generate compatible ends for Anza 20 SacI. If recognized sequnece is 5'-GTGCAC-3', then generate compatible ends for Anza 23 PstI, Anza 37 Mph1103I
IVGN1274Anza 127 RseIMslI, SmiMI CAYNNNNRTG

5'...C A Y N N^N N R T G...3'
3'...G T R N N^N N Y A C...5'
blunt
IVGN1284Anza 128 PspFIGsaICCCAGC(-1/-5)

5'…C C C A G^C...3'
3'…G^G G T C G...5'
3’ protruding ends
IVGN1294Anza 129 BshNIAccB1I, BanI, BspT107I GGYRCC

5'...G^G Y R C C...3'
3'...C C R Y G^G...5'
5’ protruding endsIf recognized sequence is 5'-GGTACC-3', then generate compatible ends for Anza 34 Pfl23II, Anza 39 Bsp1407I, Anza 91 Acc65I