The compact Thermo Scientific KingFisher Duo Prime purification system provides a flexible and user-friendly solution for automating nucleic acid and protein purification in labs with low- to medium-throughput. Combining the KingFisher Duo Prime system with MagMAX nucleic acid extraction kits allows a diverse range of sample inputs and sample types to be processed.

Either small-volume (10 µL to 50 µL) or large-volume (300 µL to 500 µL) samples can be processed on the KingFisher Duo Prime, thanks to its interchangeable magnetic rod design.

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Features of the KingFisher Duo Prime include:

  • Easy installation (ready to run in 10 minutes or less)
  • Flexible configuration includes both 6-pin and 12-pin magnetic heads to process different sample numbers and volumes
  • Preloaded programs to perform protocols with MagMAX nucleic acid isolation kits
  • Thermo Scientific BindIt Software, which allows programmable instrument control and protocol modification, creation, and upload (Windows 7 and 8 compatible)
  • New or edited protocols can be uploaded with a USB stick
  • UV lamp for decontamination and optional barcode reader provide enhanced usability

Video: Primed for performance

How It Works

The KingFisher Duo Prime magnetic particle processor utilizes permanent magnetic rods and disposable tip combs with independent movement control. When the magnetic rod—sheathed inside the tip comb—is lowered into the solution, magnetic beads collect at the bottom of the tip comb. The tip comb can then be positioned in a different well or plate, and the beads are then released by moving the magnetic rods out of the tip comb. The tip comb also facilitates the mixing of reagents with the beads as the magnetic head moves up and down.

MagMAX kits and the KingFisher Duo Prime system

When MagMAX kits are used on the KingFisher Duo Prime or KingFisher Flex system, labs will have a simple, fast system that is designed to provide pure nucleic acids ready for downstream applications such as real-time PCR and next-generation sequencing.

Top benefits of running the MagMAX kits on the KingFisher Flex processor with 24 Deep-Well Head:

  • Nucleic acid extraction from larger sample input volumes
  • Consistent, high-quality nucleic acid recovery from a wide variety of sample types
  • Cross-contamination control, thanks to bead transfer technology

Choose the right MagMAX kits for your needs

If your KingFisher instrument does not contain the required MagMAX program, please email

Dynabeads immunoprecipitation

Dynabeads products pioneered biomagnetic technology and ensure that you get the best balance of high yield and reproducibility with low nonspecific binding and cost. This balance is one reason why Dynabeads products have become the gold standard for immunoprecipitation using magnetic beads  and are well suited for automation. Here are many other reasons:

  • Low background—little to no nonspecific binding, and no preclearing and high signal to noise ratio
  • Highly reproducible—uniform beads help ensure consistent results
  • Flexible—products for IP, Co-IP, pull-down, and ChIP assays; ideal for manual and automated protocols
  • Antibody savings—all binding occurs on the smooth outer surface of the beads, which conserves precious antibodies and supports a cost-efficient solution per sample
  • Highly sensitive—Dynabeads technology is the most-cited method for sensitive applications, such as ChIP and IP of low-abundance proteins

Learn more about solutions for immunoprecipitation
Get the newest protocols for automated IP on the KingFisher platform