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Content for all—Ion AmpliSeq Panels

From off-the-shelf pre-designed panels to fully customizable panels, Ion AmpliSeq™ Panels are designed to meet the needs of any targeted sequencing research application. Not working with human research samples? Not a problem. Our Ion AmpliSeq™ Designer tool offers the ability to design custom panels from 12 predefined genomes, or from your own reference genome after uploading a reference file.

Ion AmpliSeq Technology

Ion AmpliSeq technology enables robust targeted sequencing from as little as 1 ng of FFPE DNA or RNA to simultaneously target tens to hundreds of genes. Additionally, targeted sequencing with Ion AmpliSeq Panels enables the detection of various mutation types, including SNPs, indels, and copy number variants, with a single technology.

We have also developed a database of Ion AmpliSeq predesigned fusion assays that work with limited sample input, just 1 ng of FFPE RNA, to help deliver sensitive, reliable research results of these fusion events.

Learn more about cancer research panels using the Ion AmpliSeq technology ›
Learn more about customized Ion AmpliSeq panels including gene fusions ›

Our Ion AmpliSeq inherited disease panels provide coverage of up to 751 genes, employing a straightforward and rapid PCR-based workflow requiring as little as 1 ng of input DNA.

Should a gene panel approach fail on the first pass or simply not be appropriate to your research goal, the Ion AmpliSeq Exome RDY Panel offers an exhaustive search option, covering typically >97% of protein-coding sequences. Whether you choose a focused panel or require complete exome coverage, all panels deliver exceptional coverage (>90% on-target bases) and outstanding uniformity (typically >90% bases within 20% of the mean).

Table 1.  Ion AmpliSeq Panels for inherited disease research

Ion AmpliSeq Panel Genes covered Average gene coverage Panel uniformity Percentage reads on target
Inherited Disease Panel 325 97% 91% 96%
Cardiovascular Research Panel 404 99% 90% 97%
Hematology Research Panel 394 99% 94% 97%
Neurological Research Panel 751 99% 92% 97%
Ophthalmic Research Panel 316 99% 89% 98%
Deafness Research Panel v2 128 99% 94% 95%
Dermatology Research Panel v2 222 99% 96% 96%
Dysmorphia-Dysplasia Research Panel v2 519 99% 96% 96%
Endocrine Research Panel v2 340 99% 93% 96%
Gastrointestinal Research Panel v2 194 99% 97% 95%
Inborn Errors of Metabolism Research Panel v2 594 99% 96% 97%
Primary Immune Deficiency Research Panel v2 264 99% 95% 98%
Pulmonary Research Panel v2 131 98% 96% 95%
Renal Research Panel v2 155 99% 96% 95%
Epilepsy Research Panel 386 99% 91% 98%
Autism Research Panel v2 236 99% 94% 97%
Cardiac Arrhythmias and Cardiomyopathy Research Panel 92 99% 97% 98%
Inherited Cancer Research Panel 134 99% 95% 96%
Hearing Loss Research Panel v1 63 96% 96% 91%
Dementia Research Gene Panel 17 99% 95% 87%
Noonan Research Panel 14 100% 93% 98%
TP53 Research Panel 1 100% 88% 97%
BRCA 1 & 2 Research Panel 2 100% 97% 98%
Comprehensive Ovarian Cancer Research Panel 41 99% 99% 97%
CFTR Research Panel 1 100% 98% 94%
Pharmacogenomics Research Panel 40 100% 98% 82%
Exome RDY Panel 19,072 96% 92% 90%

For full details, including gene lists and relevant publications, go to ampliseq.com.

The use of next-generation sequencing (NGS) has rapidly increased our knowledgebase on the identification and evolution of infectious disease agents. Through the use of targeted sequencing of specific genes, we are now able to efficiently identify the microbes within a mixed population, perform research on retrospective outbreak samples, study potential virulence factors and transmission patterns, and discover mutations that may be associated with antibiotic resistance.

Learn more about our family of Ion AmpliSeq Infectious Disease Research Community Panels for TB research and Ebola virus research

For research in nonhuman models and other non-model organisms, the online Ion AmpliSeq Designer tool enables custom Ion AmpliSeq Panel designs for 11 nonhuman species, using the latest reference genomes.

Should you not find your particular species of interest, upload your own custom reference genome to design a custom panel. Custom Ion AmpliSeq panels can be designed for any genome using this simple-to-use online tool.

Video: Fast Turn-Around Genotyping without a Reference Genome

Dr. James Schnable from the University of Nebraska-Lincoln has developed a technology called "tunable genotyping by sequencing" (tGBS) that provides much better read-depth compared to conventional genotyping-by-sequencing approaches.

Whether you are looking to crack tough cases involving DNA mixture samples, generate more leads for investigations, or analyze highly degraded or trace DNA, Ion AmpliSeq technology offers rapid and accurate answers in a simple and cost-effective manner with our forensically relevant Applied Biosystems™ Precision ID panels.

Learn more about Precision ID panels using Ion AmpliSeq technology