Sanger Sequencing

Sanger sequencing instruments & accessories

Our Applied Biosystems genetic analysis systems are trusted for Sanger sequencing and fragment analysis, and proven through decades of results. Our Applied Biosystems genetic analysis systems can help you obtain excellent performance, reliability, and world-class support.

Which genetic analyzer is right for you?

  Easy to use, flexible system Meets the needs of validated and process controlled environments Maximum throughput and scalability
 
  SeqStudio Genetic Analyzer 3500 Series Genetic Analyzer 3730 Series Genetic Analyzer
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Number of capillaries 4 8 (3500), 24 (3500xL) 48 (3730), 96 (3730xl)
Ease of use Simple Easy Moderate
Number of dyes 6 6 5
Capillary array length (cm) Integrated into click-in cartridge 36, 50 36, 50
RFID Yes Yes No
Polymer type Integrated into click-in cartridge POP-6, POP-7, POP-4 POP-6, POP-7
Sample capacity

12 standard 8-strip tubes
1 standard 96-well  plate 

2 sample plates (96- or 384-well) 16 sample plates (96- or 384- well)
Integrated plate stacker No No Yes
Applications Sequencing + fragment analysis on same run Sequencing, fragment analysis Sequencing, fragment analysis
Minimum run time 30 min 30 min 20 min
Sequencing read length (bp) 800 At least 850 900
Maximum sequencing throughput (base pair reads/day)  67k

138k (3500), 403k
(3500xL)

1.38 M (3730), 2.76 M
(3730xL)

Maximum fragment throughput (samples/day)**  192 384 (3500), 1,152
(3500xL)
3,456 (3730), 6,912
(3730xl)
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** Based on 20-plex microsatellites per well with optimal polymer, capillary, and run module combination

We provide a wide range of high-quality, end-to-end solutions to meet your Sanger sequencing and fragment analysis needs. We know that your results matter and are committed to manufacturing under strict quality-control standards and providing award-winning service to help you receive credible and accurate results—from sequencing reaction, to purification, capillary electrophoresis, and analysis.

The widest range of applications

Applied Biosystems genetic analyzers support the widest range of applications for sequencing and fragment analysis.
View publications

  SeqStudio
SeqStudio
3730xl
3730xL
3730
3730
3500xl
3500xL
3500
3500
3130xl
3130xL
3130
3130
310
310
  Instrument
Number of Capillaries   96 48 24 8 16 4 1
Compatible applications:
(S) Supported; (A) AB demonstrated; (C) Customer demonstrated; (N) Not supported
BAC end sequencing   S S S S S S S
Checking clone constructs S S S S S S S S
De novo sequencing S S S S S S S S
Heterozygote detection & resequenicng (SNP-based) S S S S S S S A
HLA typing   S S S S S S C
Methylation   S S S S S S C
mtDNA sequencing S S S S S S S S
Resequencing
- Comparative genomics S S S S S S S S
- Indels S S S S S S S S
SAGE method N C C C C C C A
SNP analysis S S S S S S S S
General sizing of PCR products S S S S S S S S
Fragment length polymorphism
- AFLP N S S S S S S S
- RFLP N A A A A A A A
Large fragment analysis
- BAC fingerprinting N A A A A A A N
- VNTR C C C C C C C C
Microsatellite
- Genotyping S S S S S S S S
- Analysis for forensics/HID S N N S S S S S
- Instability/RER A A A A A A A A
Conformation analysis
- SSCP N N N N N A A S
Relative fluorescent quantitation
- Loss of heterozygosity (LOH) S (MLPA) S S S S S S S
SNP genotyping
- SNaPshot system S A A S S S S S
- SNPlex system N S S S S S N N
DNA protein binding assays & DNA fingerprinting N C C C C C C C

No One Else Offers the Full Sequence

Applied Biosystems® equips all of its genetic analyzers with the same proven capillary electrophoresis technology, application-specific software, industry-leading BigDye® Terminator chemistry, and world-class support. Whether you need a single-capillary system for a few reactions a day, or a multicapillary system for a few million, we have your needs covered. And with our value-added upgrade program, you won’t lose a beat when you decide to increase your throughput.

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