Sunday 22nd May, 15:00–16:30, Room 132
Speaker: Jason Carte, Staff Scientist Molecular Biology, Thermo Fisher Scientific, Carlsbad, US
CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of cell engineering. We have developed robust tools and protocols to successfully use CRISPR-Cas9 including:
- Tools for designing and making gRNA
- Delivery to a large variety of cells including iPSCs and ESCs using lipid-mediated transfection or electroporation
- Optimised protocols for efficient editing by knock-out and knock-in
- Lentiviral and gRNA libraries
- Cell Models
Taken together, we present a streamlined cell engineering workflow that enables gRNA design to analysis of edited cells in as little as four days and results in highly efficient genome modulation in hard-to-transfect cells. The reagent preparation and delivery to cells is amendable to high throughput, multiplexed genome-wide cell engineering.
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