In this seminar, the robust and sensitive quantification of peptides using automated immunoaffinity enrichment, stable isotope labeled peptides and nanoLC-MRM-MS is outlined.
Targeted mass spectrometry is routinely employed to identify and quantify peptides and proteins in complex matrices such as tissues and plasma. Immunoaffinity enrichment of peptides coupled with analysis by stable isotope dilution multiple reaction mass spectrometry has been shown to have analytical performance and detection limits suitable for many biomarker verification studies and biological applications.
Described in this presentation is the development and performance characterization of an automated immunoaffinity workflow up to multiplex levels of 172. Also detailed is the application of a 120-plex iMRM assay to probe for protein concentration trends in plasma from patients undergoing a therapeutic planned myocardial infarction to identify biomarkers of cardiovascular disease.
Research Scientist, Broad Institute
Eric has designed and implemented enrichment strategies, such as immunodepletion, multidimensional chromatography and peptide immunoprecipitation. The application of these assays has been an integral part of biomarker discovery programs in ovarian cancer, breast cancer, rheumatoid arthritis and cardiovascular disease for more than 15 years. Eric has a BS in Biochemistry from University of New Hampshire.
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