Plant DNA Isolation kits

Plant DNA Isolation has unique challenges that require kits specifically designed to deal with carbohydrates, phenolics, and other compounds abundant in plant tissues. Plant cell walls can be very difficult to disrupt, and lysates often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA and RNA quality and inhibit downstream reactions. Life Technologies offers an expansive portfolio of kits, reagents, and devices for the isolation, purification, analysis, and quantitation not only for plant genomic DNA, but also for total RNA, mRNA, and microRNA or transcriptome RNA.

Jump to plant RNA Isolation

Which Plant DNA Isolation Kit is Right for You?

 

DNA extraction and TaqMan® chemistry-based genotyping combined, <1h to results

Magnetic beads designed to handle low-abundance plant DNA samples

Most suitable for screening and GMO testing due to automation capabilities

Slica-based method for low abundance plant DNA samples

Bulk buffers for crude extraction of DNA and RNA from plant cells (*)

The most efficient lysis for the largest amount of tissue

 

TaqMan® Sample-to-SNP™ Kit

MagMAX™ DNA Multi-Sample Kit

ChargeSwitch® gDNA Plant

PureLink™ Genomic Plant

Total RNA Lysis Solution

Plant DNAzol®

Starting Material

2-3mm punch

5-10 mg

50-100 mg

100 mg

≥ 100 mg

≥ 100 mg

Isolation technology

Lysis solution

Magnetic beads

ChargeSwitch® Chemistry, magnetic beads

Silica spin column

Bulk lysis buffer

Organic extraction

Downstream applications

Real-time PCR

All

All

All

PCR, real-time PCR

All

Automatable

yes

yes

yes

no

no

no

Yield

varies based on starting material

up to 14.6 µg

up to 7 µg

up to 14.6 µg

varies based on starting material

varies based on starting material

Protocol time

5 mins

<40 mins

<15 mins

<40 mins

<15 mins

<60 mins

Plant sample types

Works with broad rangeof plant tissues and starting material (leaf punch, needle, seed chips)

Chloroplasts and broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass &Arabidopsis thalianaleaves)

Some of the many species and tissues are canola, corn, cotton, sunflower, barley, rice (seeds); tomato, cucumber, lettuce, banana (leaves); rose, magnolia (flower); Acer, sycamore, grass, etc.

Chloroplasts and broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass andArabidopsis thalianaleaves)

Customer-validated for DNA on a broad range of plant tissues for SNP genotyping as well as corn flour for GMO testing

Broad range of plant tissues (alfalfa and sunflower sprouts, corn husks, soybeans, mushroom, tomato leaves, wheat grass andArabidopsis thalianaleaves)

High throughput compatibile

yes

yes

yes

no

yes

yes

High throughput/ 
automation options

yes

50 preps 
96 preps

ChargeSwitch® gDNA Plant 960 Kit

96-well plate

yes

yes

Kit sizes available

100 preps 
400 preps 
4000 preps

50 preps 
96 preps

96 preps 
960 preps

50 preps 
4 X 96 preps

250 mL

100 mL

 

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(*)The Total RNA Lysis solution was part of the discontinued ABI PRISM® 6100 Nucleic Acid PrepStation and is our cheapest and quickest methodology for plant DNA extraction without the use of organic solvents.


Which Plant RNA Isolation Kit is Right for You?

  Great for difficult samples (conifer tissue & seeds) Rapid & fully automated Quick & easy to use Efficient recovery of microRNA & small RNA
   Plant RNA Reagent  MagMAX™-96 Total RNA Isolation Kit  PureLink® RNA Mini Kit  mirVana™ miRNA Isolation Kit
RNA types isolated large RNA molecules only large RNA molecules only large RNA molecules only Small & large RNA molecules
Prep time 60 minutes <45 minutes <20 minutes 30 minutes
Amount of starting material Up to 1 g Up to 10 mg <250 mg 0.5 to 250 mg
Isolation method High purity organic extraction
(requires alcohol precipitation)
Scalable, flexible format with magnetic beads Fast, convenient silica column Highest purity & convenience; includes organic extraction & silica column
(no precipitation required)
High throughput compatible   yes    
Compatible products   Ambion® Plant RNA Isolation Aid
Removes common plant contaminants
Ambion® Plant RNA Isolation Aid
Removes common plant contaminants
Ambion® Plant RNA Isolation Aid
Removes common plant contaminants
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Related products

In addition to DNA and RNA isolation products, Thermo Fisher offers an expansive portfolio of solutions for analysis and quantitation of plant genomic DNA.

Resources

DNA

RNA