Rabbit monoclonal antibodies are a useful and preferred tool for scientific discovery and therapeutic antibody development [1]. They are used in many applications, including immunofluorescence, immunohistochemistry, flow cytometry, western blots, and ELISAs.

Advantages of rabbit monoclonal antibodies include [2]:

  • Natural diversity
  • High affinity and specificity
  • Novel epitope recognition
  • Cross-reactivity to human and mouse targets
  • Ease of humanization

Compared to other animal models (e.g., mouse and rat), rabbits provide these advantages and a better system for monoclonal antibody production because the rabbit immune system responds to a broader range of antigens and because rabbits have larger spleens, which produce more antibodies [2].


Search for rabbit monoclonal antibodies

  • Step 1: Search for the target of interest using the search tool below.
  • Step 2: Filter results for “Rabbit” as the host (in side bar).
  • Step 3: Filter results for “Monoclonal” (in side bar).

(Search results will also include ABfinity™ Recombinant Rabbit Monoclonal Antibodies. See below.)


Rabbit monoclonal antibody production

Rabbit monoclonal antibodies are produced by injecting an antigen into an animal to initiate a humoral immune response. Spleen cells from the immunized rabbit are then fused with myeloma cells. Finally, single-cell hybridomas (each producing a single antibody) are screened, isolated, and cultured to produce the selected antibody.

Figure 1. Rabbit monoclonal antibody production.


Consider recombinant rabbit monoclonal antibodies

See our Invitrogen™ ABfinity™ Recombinant Rabbit Monoclonal Antibodies for even greater specificity, sensitivity, and optimal performance. ABfinity monoclonal antibodies resemble rabbit monoclonals produced by hybridomas. However, because ABfinity recombinant antibodies are derived from cloned DNA sequences, they are not susceptible to cell-line drift or lot-to-lot variation.

References

1. Seeber S, Ros F, Thorey I et al. (2014) A robust high throughput platform to generate functional recombinant monoclonal antibodies using rabbit B cells from peripheral blood. PLoS One 9(3): e86184.
2. Yu G (2010) RabMAbs as an alternative source of therapeutic leads. Innovations in Pharmaceutical Technology March 2010:54–58.