Request a demo Learn more about EVOS FL Auto

Application guide: Setting up hypoxic conditions with the EVOS onstage incubator Download now

Create stunning time-lapse images in your lab

Designed specifically for the EVOS™ FL Auto Imaging System, the EVOS™ Onstage Incubator is an environmental chamber that enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and non-physiological conditions.

See it in action


EVOS FL Auto Virtual Tour

Explore the innovative design of the EVOS FL Auto Imaging System with this virtual demo.  Spin the unit around and explore the key features of the system. Try it now

Superb flexibility, ease of use, and optical performance for live cell imaging

Environmental settings and image acquisition parameters are all seamlessly integrated into the EVOS FL Auto interface, creating a high-performance inverted imaging system with superb flexibility, ease of use and optical performance for demanding live cell time-lapse imaging experiments. Features include:

  • Fully integrated environmental chamber for live cell time-lapse imaging
  • Intuitively set environmental and image acquisition parameters
  • Easily maintain physiological or non-physiological conditions with precision control
  • Small footprint, robust build, and sleek design
  • Choice of a range of vessel holders

Time-lapse videos using the EVOS FL Auto with Onstage Incubator

Cell division in HeLa cells

Cells: HeLa
Reagents: CellLight™ H2B GFP, CellLight™ Mito RFP
Time Lapse: 24 hours with images taken every 12 minutes

Imaged on EVOS FL Auto Imaging System using the Onstage Incubator

Disruption of microfilament organization in HeLa cells

Cells: HeLa treated with Latrunculin A
Reagents: CellLight™ Actin GFP, MitoTracker™ Red, NucBlue™ Live
Time Lapse: 14 hours with images taken every 5 minutes

Imaged on EVOS FL Auto Imaging System using the Onstage Incubator


Attribute Details
Compatible Vessels Well plates, 35 mm dishes, T25 Flasks
Temperature Range Ambient - 40oC
CO2 % Range 0-20%
O2 % Range Ambient - 0%
Humidity Range >80% RH @ 37oC
  • 25 x 19 x 3.7 cm (Environment chamber)
  • 37 x 16 x 20 cm (Control unit)
  • 1.5 kg (Environment chamber)
  • 10 kg (Control unit)

EVOS Onstage Incubator resources