Validated antibody panels for flow cytometry

Coined in 2010, the term “optimized multicolor immunofluorescence panel”, or OMIP, refers to a thoroughly tested and validated set of antibodies and reagents that can be used together for the multicolor characterization of a specific cell state or response. Published in the journal Cytometry Part A (Wiley Online Library), these OMIPs are designed for flow cytometry, but OMIPs may potentially be defined for image cytometry, fluorescence microscopy, and other polychromatic fluorescence–based methods.

Learn more about Optimized Multicolor Immunofluorescence Panels (OMIPS) in BioProbes 74.

Quick links to help you with your panel design

Quick links to flow cytometry training courses aimed at helping you learn more about panel design

Articles in Cytometry Part A about OMIPs are listed in the table below.

Locate the OMIP you’re interested in, using the table’s search and sort functions. Clicking on the linked text will take you to the article.

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OMIP ID OMIP name Target cell type
OMIP-001 Quality and phenotype of Ag-responsive human T cells  Human T cells
OMIP-002 Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes  Human T cells
OMIP-003 Phenotypic analysis of human memory B cells  Human B cells
OMIP-004 In-depth characterization of human T regulatory cells  Human T cells
OMIP-005 Quality and phenotype of antigen-responsive rhesus macaque T cells  Rhesus macaque T cells
OMIP-006 Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry  Human T cells
OMIP-007 A phenotypic analysis of human natural killer cells  Human NK cells
OMIP-008 Measurement of Th1 and Th2 cytokine polyfunctionality of human T cells  Human T cells
OMIP-009 Characterization of antigen-specific human T cells  Human T cells
OMIP-010 A new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples  Human hematopoietic cells
OMIP-011 Characterization of circulating endothelial cells (CECs) in peripheral blood  Human endothelial cells
OMIP-012 Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice  Human leukocytes in humanized mouse
OMIP-013 Differentiation of human T-cells  Human T cells
OMIP-014 Validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining  Human T cells
OMIP-015 Human regulatory and activated T-cells without intracellular staining  Human T cells
OMIP-016 Characterization of antigen-responsive macaque and human T-cells  Cynomolgus macaque or Human T Cells
OMIP-017 Human CD4+ helper T-cell subsets including follicular helper cells  Human T cells
OMIP-018 Chemokine receptor expression on human T helper cells  Human T cells
OMIP-019 Quantification of human γδ T-cells, iNKT-cells, and hematopoietic precursors  Human T cells, NK cells
OMIP-020 Phenotypic characterization of human γδ T-cells by multicolor flow cytometry  Human T cells
OMIP-021 Simultaneous quantification of human conventional and innate-like T-cell subsets  Human T cells
OMIP-022 Comprehensive assessment of antigen-specific human T-cell functionality and memory  Human T cells
OMIP-023 10-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes  Human leukocytes
OMIP-024 Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples Human leukocytes
OMIP-025 Evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining  Human T cells, NK cells
OMIP-026 Phenotypic analysis of B and plasma cells in rhesus macaques  Rhesus macaque B cells, plasma cells
OMIP-027 Functional analysis of human natural killer cells  Human NK cells
OMIP-028 Activation panel for rhesus macaque NK cell subsets  Rhesus macaque NK cells
OMIP-029 Human NK-cell phenotypization  Human NK cells
OMIP-030* Characterization of human T cell subsets via surface markers  Human T cells
OMIP-031* Immunologic checkpoint expression on murine effector and memory T-cell subsets  Mouse T cells
OMIP-032* Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland  Mouse leukocytes
OMIP-033* A comprehensive single step staining protocol for human T- and B-cell subsets  Human T cells, B cells
OMIP-034* Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies  Human leukocytes
OMIP-035* Functional analysis of natural killer cell subsets in macaques  Rhesus macaque NK cells
OMIP-036* Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets  Human T cells
*At the present time this article is only available by purchase.