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Within the Cell Culture & Transfection Learning Center, the Resource Library is where you can connect to all our different educational, technical, application, and other learning materials by resource collection. This includes the application notes, case studies, videos, webinars, and white papers.

To browse learning content by cell culture & transfection application subtopic, visit the main Cell Culture & Transfection Learning Center home.

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Gibco Education virtual labs

The Gibco Education virtual labs represent a unique interactive cell culture training experience. Learn everything from the fundamentals of cell culture to key techniques for primary and stem cell cultures and successful protein expression. As you progress through each lab, you can certify your knowledge by taking in-lab quizzes.


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Cell Culture & Transfection Application Notes

Cell Culture & Transfection application notes present research applications and proof-of-concept experiments involving various products from our cell biology portfolio, including Thermo Scientific™, Invitrogen™ and Gibco™ products.

Blog: Advancing Cell Culture

Advancing Cell Culture is your your source for cell culture news and best practices. We're here to help—with informative resources for everything from cell isolation and growth to cell analysis, harvesting and storage so you can stay confidently on track.

Cell Culture & Transfection Support Center

Browse the support centers by application for product and general technical support. Includes resources and tips for getting started, and troubleshooting help all in one location.


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Type Name Category
Application note 5 Reasons to enhance your cell culture using variable oxygen control  CO2 incubation
Application note Application properties of materials used for porous membranes in cell culture inserts  Cultureware
Application note Aspects of cryo preservation  Cryopreservation
Application note Back to basics: Proper care and maintenance for your cell culture incubator  CO2 incubation
Application note Biological safety cabinet filter testing—Lessons from overseas  Biological safety cabinets
Application note Cell culture contamination: Evaluating contamination prevention solutions for the CO2 incubator  CO2 incubation
Application note Cell culture contamination: The role of the CO2 incubator in reducing contamination  CO2 incubation
Application note Cell culture contamination: Understanding the causes and managing the risks  General cell culture
Application note Cell harvesting by temperature reduction—Nunc UpCell Surface  Cultureware
Application note Choosing the right orbital shaker for your application  Orbital shakers
Application note Compatibility of mounting media on Nunc Permanox Slides  Cultureware
Application note Cryopreservation of mammalian cells  Cryopreservation
Application note Culturing HEL 299 Cell Line on Nunclon Delta Surface  Cultureware
Application note Culturing L929 Cell Line on Nunclon Delta Surface  Cultureware
Application note Culturing primary chick embryo cells on Nunclon Delta Surface  Cultureware
Application note Culturing V79-4 cell line on Nunclon Delta Surface  Cultureware
Application note Culturing cells under hypoxic conditions for biologically relevant results  Filtration
Application note Culturing embryonic stem cells using media filtered with Thermo Scientific Nalgene Rapid-Flow PES filter units  Filtration
Application note Decontamination cycles in BBD 6220 and Heracell incubators completely eliminate Mycoplasma
CO2 incubation
Application note Delivery solutions for immune cells Transfection
Application note Does a larger fluid reservoir give me more cooling capacity and improved temperature stability?  ENESDE  Baths, circulators, chillers
Application note Does laboratory filtration of culture media affect cell growth?  Filtration
Application note Effective heat sterilization in CO2 incubators  CO2 incubation
Application note Eliminate incubator contamination with Thermo Scientific Heracell incubators  CO2 incubation
Application note Ergonomics and safety in biological research cabinets   Biological safety cabinets
Application note Feeder cell and extracellular matrix-free cultivation of hPSC on Nunclon Vita with Rho-kinase inhibitor  Cultureware
Application note Flow rate and throughput of cell culture media and serum using Nalgene Rapid-Flow filters with 0.2 micron PES membrane  Cultureware
Application note Generation and transfection of induced pluripotent stem cells (iPSCs) Transfection
Application note How can a water-cooled condenser option significantly reduce energy consumption and heat output without compromising sample protection?  Cold storage
Application note How can blood banks eliminate potential variables to ensure consistent product yield and reproducibility in centrifugation?  Centrifugation
Application note How can blood banking and bioprocessing production facilities enhance ergonomics, while simplifying operation for greater productivity during their separations?  Centrifugation
Application note How can I help improve safety in my laboratory when spinning pathogens or patient samples in swing-out rotors?  Centrifugation
Application note How can instant rotor identification enhance productivity and safety during your centrifuge set-up and run?  Centrifugation
Application note How can using the wrong type of water to provide in-chamber humidity cause corrosion in my CO2 incubator?  CO2 incubation
Application note How can you extend the filter life of your BSC?  Biological safety cabinets
Application note How do carbon fiber rotors ensure greater sample integrity than metal rotors during the sample preparation process?  Centrifugation
Application note How do I achieve the best (fastest) time-to-temperature from a heated bath circulator?  Baths, circulators, chillers
Application note How do I ensure that my heated bath circulator will be able to control to that temperature rate?  Baths, circulators, chillers
Application note How do I select an energy efficient refrigerated bath circulator?  Baths, circulators, chillers
Application note How does a CO2 resistant shaker provide greater reliability, flexibility and ease of use for varied cell culture applications, compared to a dedicated incubated CO2 shaker?  Orbital shakers
Application note How does a low-binding culture surface compare to a non-treated surface in supporting cancer spheroid cultures?  Cultureware
Application note How does Thermo Scientific Auto-Lock rotor exchange facilitate rotor placement and improve safety, compared with traditional rotor tie-down systems?  EN DE Centrifugation
Application note How is it possible to reduce energy consumption and environmental impact with production-level centrifuges?  Centrifugation
Application note How will the cost of purchasing and running a recirculating chiller or bath circulator affect my bottom line?  Baths, circulators, chillers
Application note I need an ultra-low freezer to store my samples. Does the initial pull down time of a freezer indicate how well the unit can protect my samples after a door opening?  Cold storage
Application note I plan on using my bath circulator for external circulation only. Should I get a large reservoir that can absorb the heat for more stability?  EN ES DE  Baths, circulators, chillers
Application note I want to keep my samples at a temperature of 37°C. Which type of water bath is ideal for this requirement: general purpose, circulating or shaking?  Baths, circulators, chillers
Application note I want to use a bath circulator to immerse my vessels. How do I select the right size bath for my application?  EN ES DE  Baths, circulators, chillers
Application note I’m hearing a lot about SNAP in my lab. What is SNAP?  Cold storage
Application note Importance of Class 100 air in a CO2 incubator  CO2 incubation
Application note Improve genome editing outcomes in biologically relevant cell models Transfection
Application note Improve lentiviral production using Lipofectamine 3000 reagent Transfection
Application note Intrawell cell distribution in MicroWell Edge Plates  Cultureware
Application note iPSC generation using Lipofectamine transfection reagent  Transfection
Application note Is my -80°C freezer designed to prepare samples for long-term storage?  Cold storage
Application note Is the use of hydrocarbon refrigerants in laboratory refrigerators and freezers safe?  Cold storage
Application note Is there a safer way to store potentially flammable, pharmaceutical-grade materials that require refrigerated or frozen temperatures?  Cold storage
Application note Isolation of mitochondria using the Thermo Scientific Sorvall LYNX 6000 superspeed centrifuge  Centrifugation
Application note I’ve heard that switching to −70°C for ultra-low freezers can reduce energy, but is that safe for my sample?  Cold storage
Application note Large-scale recombinant protein production using the Thermo Scientific Sorvall LYNX 6000 Superspeed Centrifuge and Fiberlite Large Capacity Rotors  Centrifugation
Application note Large volume precipitation of proteins with ammonium sulfate using Thermo Scientific Fiberlite Carbon Fiber Rotors  Centrifugation
Application note Neurobiology transfection guide Transfection
Application note Optimizing neuron adhesion and growth by choosing the right surface of Thermo Scientific Chamber Slide  General cell culture
Application note Oxygen: Too much of a good thing?  CO2 incubation
Application note Pelleting efficiency of patented tiltied adapters using the new Thermo Scientific benchtop 1L centrifuges: a comparison of pelleting in Thermo Scientific benchtop and superspeed floor-model centrifuges  Centrifugation
Application note pH and pressure issues in closed cell culture vessels  Cultureware
Application note Proper care and maintenance of your cell culture incubator  CO2 incubation
Application note Reduced evaporation in Nunc Edge plate with solid reservoir  Cultureware
Application note Separation of PBMCs from blood samples using the new Thermo Scientific benchtop 1L centrifuge  Centrifugation
Application note Stem cell transfection guide Transfection
Application note Superior imaging quality of Nunc Optical Bottom Plate  Cultureware
Application note The Thermo Scientific Carrier Plate enables precise adjustment of height for Cell Culture Inserts in multiwell dishes  Cultureware
Application note The Thermo Scientific Nunclon Sphera surface supports effective formation of three dimensional cancer spheroids in suspension  Cultureware
Application note Thermo Scientific Biosafety: A European view  Biological safety cabinets
Application note Thermo Scientific Controlled Environment Biological Safety Cabinets: Controlling contamination  Biological safety cabinets
Application note Thermo Scientific Nunc TripleFlask culturing technique  Cultureware
Application note Total thermal disinfection of CO2 incubators  CO2 incubation
Application note Uneven cell growth during incubation  Cultureware
Application note University of Michigan field study of Class II Biological Safety Cabinet energy consumption costs  Biological safety cabinets
Application note What steps can I take to ensure that my heated bath circulator can actually bring my application to setpoint temperature?  Baths, circulators, chillers
Application note What do I need to consider when preparing samples in bags using a controlled-rate freezer?  Cold storage
Application note What kind of storage tube should I use to prevent the breakdown of RNA in tissue during cold storage?  Cold storage
Application note What technologies make it possible to reduce energy consumption and environmental impact with high performance floor model centrifuges?  Centrifugation
Application note Why should you select a Class II Biological Safety Cabinet (BSC) with a DC motor over one with an AC motor?  Biological safety cabinets
Application note Where is the highest risk of contamination in your biological safety cabinet (BSC): the HEPA filters or the airflow balance at the front opening?  Biological safety cabinets
Application note Which incubation parameters are most important for proper cell growth and expression?  CO2 incubation
Application note Why are airflow alarms an important consideration for biological safety cabinets (BSCs)  Biological safety cabinets
Application note Why does the location of sensors in my CO2 incubator affect responses from my cultured cells?  CO2 incubation
Application note Why is a circulating fan essential to superior growth conditions in a CO2 cell culture incubator?  CO2 incubation
Application note Why is a circulating fan essential to superior growth conditions in a cell culture incubator?  CO2 incubation
Application note Why is a dual blower system better than a single blower system?  Biological safety cabinets
Application note Why is automated heat decontamination a better solution for sterilizing your CO2 incubator than an automated chemical disinfection?  CO2 incubation
Article Innovations in cell culture technology drive drug discovery studies  Cultureware
Blog Advancing Cell Culture General cell culture
Citation Cytotoxicity testing of burn wound dressings, ointments and creams: a method using polycarbonate cell culture inserts on a cell culture system  Cultureware
Citation Optimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate  Cultureware
Citation Pro-apoptotic molecule suppression using siRNA delivery  Transfection
Handbook A guide for proper cryogenic preservation  Cryopreservation
Handbook Gibco Cell Culture Basics Handbook General cell culture
Handbook Pluripotent Stem Cell Handbook General cell culture
Handbook Primary Cell Culture Sourcebook  General cell culture
Handbook Protein Expression Handbook General cell culture
Handbook Skin and Eye Primary Cell Sourcebook  General cell culture
Handbook Transfection and Genome Engineering Handbook  Transfection
Mobile app Gibco Cell Culture Companion  General cell culture
Protocol 3D cell culture protocols General cell culture
Protocol Cell culture protocols General cell culture
Protocol Neural cell culture protocols General cell culture
Protocol Primary cell protocols General cell culture
Protocol Serum-free media protocols General cell culture
Protocol Stem cell protocols General cell culture
Protocol Transfecting neural cells using Neon Transfection System Transfection
Protocol Transfection protocols Transfection
Protocol Transfection protocols (by cell type) Transfection
Protocol Transfection protocols (by reagent) Transfection
Selection guide Lipofectamine transfection reagent selection guide Transfection
Selection guide Nunc cell culture surface selection guide  Cultureware
Selection guide Nunc microplate selection guide  Cultureware
Selection guide Primary cell media & supplement selection guide General cell culture
Selection guide Selection guide for CRISPR delivery systems Transfection
Selection guide iMATCH sera lot matching tool General cell culture
Video Advantages of FluoroBrite DMEM for cell imaging  General cell culture
Video Advantages of Neon Transfection System  Transfection
Video Freezing cells  Cryopreservation
Video Handling and storing your primary cells  General cell culture
Video How to adapt pluripotent stem cells from other media into Essential 8 Medium  General cell culture
Video How to passage pluripotent stem cells in Essential 8 Medium  General cell culture
Video How to prepare Essential 8 Medium for pluripotent stem cell culture  General cell culture
Video How to prepare vitronectin substrate plates for pluripotent stem cell culture  General cell culture
Video How to stain pluripotent stem cells with Alkaline Phosphatase Live Stain  Cell analysis
Video How to use the TaqMan® hPSC Scorecard Panel plates to evaluate pluripotent stem cells  General cell culture
Video Introduction to cell culture  General cell culture
Video Lipofectamine MessengerMAX for efficient mRNA transfection  Transfection
Video Passaging cells  General cell culture
Video Performing electroporation with the Neon Transfection System  Transfection
Video Sterile technique  General cell culture
Video Subculturing (or passaging) your primary cells  General cell culture
Video Thawing and plating your primary cells  General cell culture
Video Thawing cells  General cell culture
Video Why does the price of FBS fluctuate constantly?  General cell culture
Webinar Avoid the icebergs! Managing sample preparation for cryo storagenbsp; Cryopreservation
Webinar Best practices in cell counting for accurate and reproducible data  Cell analysis
Webinar Best practices for incubating sensitive cells  General cell culture
Webinar Cell culture contamination: What you don't know can cost you  General cell culture
Webinar Choosing the ideal lab water system for your cell culture applications  General cell culture
Webinar Choosing the right cold storage for cell culture applications  Cryopreservation
Webinar Culture your cells using less oxygen for biologically relevant results  General cell culture
Webinar Eliminate the threat: Cleaning and contamination in your biological safety cabinet  Biological safety cabinet
Webinar From the hood to the microscope—Revolutionizing cell-based imaging  Cell analysis
Webinar Get a handle on liquid handling. Increase pipetting efficiency for reproducible results  General cell culture
Webinar Harnessing new dimensions in your research: Growing 3D cultures consistently and repeatedly  General cell culture
Webinar How to boost your CRISPR editing efficiency  Transfection
Webinar Maintaining integrity: Selecting the right storage tube for downstream cell culture  Cryopreservation
Webinar No more separation anxiety! A research case study: Gentle, non-enzymatic detachment of sensitive cells  General cell culture
Webinar Nurture your incubator; nurture your cells. Proper care and “feeding” of your cell culture incubator  CO2 Incubation
Webinar Optimize your cell culture workflow with Thermo Scientific peristaltic pumps  General cell culture
Webinar ;Optimizing results in your downstream imaging applications  Cell analysis
Webinar Protect you and your science—with biological safety cabinets  Biological safety cabinets
Webinar Surface selection: Achieving optimal growth and harvesting of your cells  Cultureware
Webinar Take your samples for a spin: Fundamentals of cell culture centrifugation  General cell culture
Webinar Take your cell-based assays to the edge; reducing edge-effects for full-plate results  Cultureware
Webinar The last line of defense: cell culture contamination and using filtration as a final preventative measure  General cell culture
Webinar The power behind culturing cells on a porous membrane  Cultureware
Webinar The seven keys to safeguarding your cryopreserved cells  Cryopreservation
Webinar Understanding all incubation parameters for sensitive cell growth  CO2 Incubation
Webinar Versatile is smart! The power behind culturing cells on a porous membrane  Cultureware