Research applications and techniques for cell culture

Cell Culture & Transfection application notes present research applications and proof-of-concept experiments involving various products from our cell biology portfolio, including Thermo Scientific, Invitrogen and Gibco products.

If you're looking to learn about methods and techniques to achieve improved transfection and cell culture results, tested protocols, or descriptions of various techniques (and their tradeoffs), you are likely to find such answers among these application notes.


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Title Application
 Harnessing new dimensions in your research: coming ’round to spheroid culture 3D culture
 I want to keep my samples at a temperature of 37°C. Which type of water bath is ideal for this requirement: general purpose, circulating or shaking? Baths, circulators, chillers
 I want to use a bath circulator to immerse my vessels. How do I select the right size bath for my application? EN | ES | DE Baths, circulators, chillers
 How do I select an energy efficient refrigerated bath circulator? Baths, circulators, chillers
 How do I ensure that my heated bath circulator will be able to control to that temperature rate? Baths, circulators, chillers
 How do I achieve the best (fastest) time-to-temperature from a heated bath circulator? Baths, circulators, chillers
 What steps can I take to ensure that my heated bath circulator can actually bring my application to setpoint temperature? Baths, circulators, chillers
 Does a larger fluid reservoir give me more cooling capacity and improved temperature stability? EN | ES | DE Baths, circulators, chillers
 How will the cost of purchasing and running a recirculating chiller or bath circulator affect my bottom line? Baths, circulators, chillers
 I plan on using my bath circulator for external circulation only. Should I get a large reservoir that can absorb the heat for more stability? EN | ES | DE Baths, circulators, chillers
 Thermo Scientific Controlled Environment Biological Safety Cabinets: Controlling contamination Biological safety cabinets
 University of Michigan field study of Class II Biological Safety Cabinet energy consumption costs Biological safety cabinets
 Ergonomics and safety in biological research cabinets Biological safety cabinets
 Why is a dual blower system better than a single blower system? Biological safety cabinets
 Thermo Scientific Biosafety: A European view Biological safety cabinets
 How can you extend the filter life of your BSC? Biological safety cabinets
 Why should you select a Class II Biological Safety Cabinet (BSC) with a DC motor over one with an AC motor? Biological safety cabinets
 Where is the highest risk of contamination in your biological safety cabinet (BSC): the HEPA filters or the airflow balance at the front opening? Biological safety cabinets
 Why are airflow alarms an important consideration for biological safety cabinets (BSCs) Biological safety cabinets
 Biological safety cabinet filter testing—Lessons from overseas Biological safety cabinets
 How is it possible to reduce energy consumption and environmental impact with production-level centrifuges? Centrifugation
 How can I help improve safety in my laboratory when spinning pathogens or patient samples in swing-out rotors? Centrifugation
 How can blood banks eliminate potential variables to ensure consistent product yield and reproducibility in centrifugation? Centrifugation
 How can instant rotor identification enhance productivity and safety during your centrifuge set-up and run? Centrifugation
 Separation of PBMCs from blood samples using the new Thermo Scientific benchtop 1L centrifuge Centrifugation
 How do carbon fiber rotors ensure greater sample integrity than metal rotors during the sample preparation process? Centrifugation
 How can blood banking and bioprocessing production facilities enhance ergonomics, while simplifying operation for greater productivity during their separations? Centrifugation
 Large volume precipitation of proteins with ammonium sulfate using Thermo Scientific Fiberlite Carbon Fiber Rotors Centrifugation
 Large-scale recombinant protein production using the Thermo Scientific Sorvall LYNX 6000 Superspeed Centrifuge and Fiberlite Large Capacity Rotors Centrifugation
 Isolation of mitochondria using the Thermo Scientific Sorvall LYNX 6000 superspeed centrifuge Centrifugation
 How does Thermo Scientific Auto-Lock rotor exchange facilitate rotor placement and improve safety, compared with traditional rotor tie-down systems? EN | DE Centrifugation
 Pelleting efficiency of patented tiltied adapters using the new Thermo Scientific benchtop 1L centrifuges: a comparison of pelleting in Thermo Scientific benchtop and superspeed floor-model centrifuges Centrifugation
 What technologies make it possible to reduce energy consumption and environmental impact with high performance floor model centrifuges? Centrifugation
 Why does the location of sensors in my CO2 incubator affect responses from my cultured cells? CO2 incubation
 Eliminate incubator contamination with Thermo Scientific Heracell incubators CO2 incubation
 Importance of Class 100 air in a CO2 incubator CO2 incubation
 Effective heat sterilization in CO2 incubators CO2 incubation
 5 Reasons to enhance your cell culture using variable oxygen control CO2 incubation
 Which incubation parameters are most important for proper cell growth and expression? CO2 incubation
 Proper care and maintenance of your cell culture incubator CO2 incubation
 Decontamination cycles in BBD 6220 and Heracell incubators completely eliminate Mycoplasma CO2 incubation
 How can using the wrong type of water to provide in-chamber humidity cause corrosion in my CO2 incubator? CO2 incubation
 Why is automated heat decontamination a better solution for sterilizing your CO2 incubator than an automated chemical disinfection? CO2 incubation
 Cell culture contamination: The role of the CO2 incubator in reducing contamination CO2 incubation
 Cell culture contamination: Evaluating contamination prevention solutions for the CO2 incubator CO2 incubation
 Total thermal disinfection of CO2 incubators CO2 incubation
 Why is a circulating fan essential to superior growth conditions in a CO2 cell culture incubator? CO2 incubation
 Oxygen: Too much of a good thing? CO2 incubation
 Why is a circulating fan essential to superior growth conditions in a cell culture incubator? CO2 incubation
 Back to basics: Proper care and maintenance for your cell culture incubator CO2 incubation
 I’m hearing a lot about SNAP in my lab. What is SNAP? Cold storage
 I’ve heard that switching to −70°C for ultra-low freezers can reduce energy, but is that safe for my sample? Cold storage
 I need an ultra-low freezer to store my samples. Does the initial pull down time of a freezer indicate how well the unit can protect my samples after a door opening? Cold storage
 What do I need to consider when preparing samples in bags using a controlled-rate freezer? Cold storage
 What kind of storage tube should I use to prevent the breakdown of RNA in tissue during cold storage? Cold storage
 How can a water-cooled condenser option significantly reduce energy consumption and heat output without compromising sample protection? Cold storage
 Is my -80°C freezer designed to prepare samples for long-term storage? Cold storage
 Is the use of hydrocarbon refrigerants in laboratory refrigerators and freezers safe? Cold storage
 Is there a safer way to store potentially flammable, pharmaceutical-grade materials that require refrigerated or frozen temperatures? Cold storage
 Cryopreservation of mammalian cells Cryopreservation
 Aspects of cryo preservation Cryopreservation
 Edge 2.0 Plate Application Note Cultureware
 pH and pressure issues in closed cell culture vessels Cultureware
 Intrawell cell distribution in MicroWell Edge Plates Cultureware
 Application properties of materials used for porous membranes in cell culture inserts Cultureware
 How does a low-binding culture surface compare to a non-treated surface in supporting cancer spheroid cultures? Cultureware
 Uneven cell growth during incubation Cultureware
 Cell harvesting by temperature reduction—Nunc UpCell Surface Cultureware
 Feeder cell and extracellular matrix-free cultivation of hPSC on Nunclon Vita with Rho-kinase inhibitor Cultureware
 Compatibility of mounting media on Nunc Permanox Slides Cultureware
 Culturing HEL 299 Cell Line on Nunclon Delta Surface Cultureware
 Reduced evaporation in Nunc Edge plate with solid reservoir Cultureware
 Culturing L929 Cell Line on Nunclon Delta Surface Cultureware
 Superior imaging quality of Nunc Optical Bottom Plate Cultureware
 The Thermo Scientific Carrier Plate enables precise adjustment of height for Cell Culture Inserts in multiwell dishes Cultureware
 The Thermo Scientific Nunclon Sphera surface supports effective formation of three dimensional cancer spheroids in suspension Cultureware
 Culturing V79-4 cell line on Nunclon Delta Surface Cultureware
 Thermo Scientific Nunc TripleFlask culturing technique Cultureware
 Culturing primary chick embryo cells on Nunclon Delta Surface Cultureware
 Flow rate and throughput of cell culture media and serum using Nalgene Rapid-Flow filters with 0.2 micron PES membrane Cultureware
 Culturing embryonic stem cells using media filtered with Thermo Scientific Nalgene Rapid-Flow PES filter units Filtration
 Culturing cells under hypoxic conditions for biologically relevant results Filtration
 Does laboratory filtration of culture media affect cell growth? Filtration
 Optimizing neuron adhesion and growth by choosing the right surface of Thermo Scientific Chamber Slide General cell culture
 Cell culture contamination: Understanding the causes and managing the risks General cell culture
 Choosing the right orbital shaker for your application Orbital shakers
 How does a CO2 resistant shaker provide greater reliability, flexibility and ease of use for varied cell culture applications, compared to a dedicated incubated CO2 shaker? Orbital shakers
Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemFlex Medium Transfection
Transfection of pluripotent stem cells with Lipofectamine Stem reagent in mTeSR1 Medium on Geltrex matrix Transfection
Transfection of neural stem cells with Lipofectamine Stem Transfection Reagent in StemPro medium Transfection
 iPSC generation using Lipofectamine transfection reagent Transfection
Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in Essential 8 Medium Transfection
Delivery solutions for immune cells Transfection
Neurobiology transfection guide Transfection
Improve genome editing outcomes in biologically relevant cell models Transfection
Generation and transfection of induced pluripotent stem cells (iPSCs) Transfection
Improve lentiviral production using Lipofectamine 3000 reagent Transfection