Bolt-Bis-Tris Plus gels

Bolt Bis-Tris Plus Gels are precast polyacrylamide gels developed to provide optimal separation of a wide range of protein sizes under denaturing conditions. Bolt Bis-Tris Plus Gels are designed to deliver consistent gel performance with a neutral pH environment that minimizes protein modifications. As such, Bolt gels are a good choice before downstream transfer and western blot analysis and any other technique where protein integrity is crucial.

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Available Bolt Bis-Tris Plus Gels
Migration charts

Bolt Bis-Tris Plus Gels are precast polyacrylamide gels developed to provide optimal separation of a wide range of protein sizes under denaturing conditions. Bolt Bis-Tris Plus Gels are designed to deliver consistent gel performance with a neutral pH environment that minimizes protein modifications. As such, Bolt gels are a good choice before downstream transfer and western blot analysis and any other technique where protein integrity is crucial.

Features of Bolt Bis-Tris Plus Gels include:

  • Better protein integrity— preserves your proteins with neutral pH sample preparation process
  • Optimized run conditions—separates your proteins in approximately 35 minutes using constant voltage
  • Wedge-shaped wells—helps you achieve easier sample loading and up to two times higher sample volume
  • Long shelf life—allows you to store gels for at least 16 months at 4–25°C

Bolt Bis-Tris Plus Gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use buffers containing SDS. The gels can be run using MES SDS running buffer to better resolve smaller proteins (0.3–260 kDa) and MOPS SDS running buffer to resolve medium- to large-size proteins (14–260 kDa). 

Bolt Bis-Tris Plus Gels use a bis-tris discontinuous buffer system involving three ions:

  • Chloride (–) is from the gel buffer and serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are bis-tris (+) and Cl (–) (pH 6.4).
  • MES or MOPS (–) serves as the trailing ion in the running buffer. The running buffer ions are tris (+), MOPS/MES (–) and dodecylsuflate (pH 7.3–7.7).
  • Bis-tris (+) is the common ion present in the gel buffer and running buffer. The combination of lower pH gel buffer (pH 6.4) and the running buffer (pH 7.3–7.7) results in a significantly lower operating pH of 7 during electrophoresis.
Available gel sizes Mini: 8 cm x 8 cm (1.0 mm thick)
Storage conditions Room temperature
Shelf life 16 months
Recommended sample buffer LDS sample buffer
Recommended running buffers MES or MOPs running buffer
Gel chemistry Bis-tris
Available polyacrylamide concentrations 8%, 10%, 12% and 4–12%
Separation range (denaturing) 0.3–260 kDa
For use with (equipment) Mini Gels Mini Gel Tank or XCell SureLock Mini-Cell
Mode of separation Molecular weight
Well type 1D, Invitrogen WedgeWell format

Bolt Bis-Tris Plus Gels are precast polyacrylamide gels developed to provide optimal separation of a wide range of protein sizes under denaturing conditions. Bolt Bis-Tris Plus Gels are designed to deliver consistent gel performance with a neutral pH environment that minimizes protein modifications. As such, Bolt gels are a good choice before downstream transfer and western blot analysis and any other technique where protein integrity is crucial.

Features of Bolt Bis-Tris Plus Gels include:

  • Better protein integrity— preserves your proteins with neutral pH sample preparation process
  • Optimized run conditions—separates your proteins in approximately 35 minutes using constant voltage
  • Wedge-shaped wells—helps you achieve easier sample loading and up to two times higher sample volume
  • Long shelf life—allows you to store gels for at least 16 months at 4–25°C

Bolt Bis-Tris Plus Gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use buffers containing SDS. The gels can be run using MES SDS running buffer to better resolve smaller proteins (0.3–260 kDa) and MOPS SDS running buffer to resolve medium- to large-size proteins (14–260 kDa). 

Bolt Bis-Tris Plus Gels use a bis-tris discontinuous buffer system involving three ions:

  • Chloride (–) is from the gel buffer and serves as a leading ion due to its high affinity to the anode relative to other anions in the system. The gel buffer ions are bis-tris (+) and Cl (–) (pH 6.4).
  • MES or MOPS (–) serves as the trailing ion in the running buffer. The running buffer ions are tris (+), MOPS/MES (–) and dodecylsuflate (pH 7.3–7.7).
  • Bis-tris (+) is the common ion present in the gel buffer and running buffer. The combination of lower pH gel buffer (pH 6.4) and the running buffer (pH 7.3–7.7) results in a significantly lower operating pH of 7 during electrophoresis.
Available gel sizes Mini: 8 cm x 8 cm (1.0 mm thick)
Storage conditions Room temperature
Shelf life 16 months
Recommended sample buffer LDS sample buffer
Recommended running buffers MES or MOPs running buffer
Gel chemistry Bis-tris
Available polyacrylamide concentrations 8%, 10%, 12% and 4–12%
Separation range (denaturing) 0.3–260 kDa
For use with (equipment) Mini Gels Mini Gel Tank or XCell SureLock Mini-Cell
Mode of separation Molecular weight
Well type 1D, Invitrogen WedgeWell format