Amine Biotinylation Reagents
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
NHS-esters of biotin are water-insoluble and membrane-permeable because they do not possess a charged group. They may be used for biotinylating internal as well as external cellular components.
Sulfo-NHS-esters are water soluble and useful for applications that cannot tolerate organic solvents. Sulfo-NHS-esters of biotin are recommended for use as cell surface biotinylation reagents. Because of the charged sulfonate group, these compounds do not penetrate the plasma membrane, thus sulfo-NHS-esters biotinylation is restricted to the cell surface.
Choose the best amine-reactive biotinylation kit for your experiment
|Kit categories||Product||Product features||Spacer arm (Å)||Water soluble?||Cleavable?|
|NHS-PEG4-Biotin and Biotinylation Kits||PEG4-Biotin transfer and label amines, and maximize the solubility of antibodies and other proteins||29||Yes||No|
|Sulfo-NHS-Biotin and Biotinylation Kits||Complete kit with a fast and efficient procedure for labeling a protein and determining how much biotin has been attached||13.5||Yes||No|
|Sulfo-NHS-LC-Biotin and Biotinylation Kits||Complete kit with an optimized procedure for labeling a protein and determining how much biotin has been attached||22.4||Yes||No|
|Sulfo-NHS-SS-Biotin and Biotinylation Kits||
Cleavable biotinylation reagent allows for later removal of the biotin label
|NHS-PEG Solid Phase Biotinylation Kits||A column-based approach to antibody biotinylation and clean-up||N/A||N/A||N/A|
For Research Use Only. Not for use in diagnostic procedures.