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Next-Generation Sequencing and Liquid Biopsy Applications


Ion Torrent Ion S5 and Ion S5 XL NGS Systems for Targeted Sequencing

Adopting next-generation sequencing (NGS) in your lab is now simpler than ever. The Ion S5™ and Ion S5™ XL Systems enable the simplest targeted sequencing workflow for your lab with industry-leading speed and affordability.

  • DNA to data with less than 45 minutes of hands-on time—with simple cartridge-loaded reagents and a straightforward user interface, the Ion S5 and Ion S5 XL Systems make NGS fast and easy—ideal for any.
  • Single sequencer—multiple applications—each Ion S5 and Ion S5 XL System currently supports three different chip types, so you can run multiple applications on a single sequencer. 
  • Because every hour counts—get results in as little as 24 hours (with the Ion Chef™ and 2.5-4 hour sequencer run times) using the Ion S5 XL System—the fastest turnaround of any benchtop sequencer. 

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Oncomine Assays

The Ion Torrent Oncomine Comprehensive Assay is a targeted assay for the detection of cancer gene variants to inform clinical trial research programs.

The Ion Torrent™ Oncomine™ Focus Assay enables detection of variants in 52 solid tumor genes that are associated with current oncology drugs and published evidence. Designed using the Oncomine™ Knowledgebase, a rich cancer genomics data resource for target selection, the Oncomine assays offer:

  • NGS sequencing method requiring minimal tumor sample input per run
  • Analysis of thousands of relevant biomarkers using a single workflow for DNA and RNA
  • Workflow with fast turnaround time

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Oncomine Knowledgebase Reporter

Sample-specific reports based on curated, evidence-based content from around the world

The Oncomine™ Knowledgebase Reporter is a curated set of published evidence from clinical trials that supports the matching of driver genetic variants with relevant potential clinical therapeutic options, such as published therapeutic interventions and clinical trials.

  • One curated resource of published evidence from global data sources
  • Streamlined access to a sample-specific variant report in only three steps
  • Customizable templates for your laboratory

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LiquidBiopsy Platform

Accurately profile mutations of interest in cell-free DNA and circulating tumor cells

The LiquidBiopsy™ Platform enables the molecular analysis of CTC, cfDNA, and germline nucleic acids from a single blood sample. Achieving a high recovery rate for both CTC and cell-free genomic DNA, and exceptional purity, the platform enables downstream molecular characterization of genomic content using the Ion S5 System; or Ion PGM System. The LiquidBiopsy Platform:

  • Offers a comprehensive, cost-effective sample-to-sequence workflow
  • Is uniquely suited to low-input DNA sequencing without the need for whole-genome amplification
  • Enables highly sensitive multiplexing
  • Achieves 1% limit of detection, critical to liquid biopsy research
  • Enables rapid turnaround time: sample-to-sequence workflow typically completed in 2.5 days

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Ion AmpliSeq Technology

Delivers simple and fast library construction

Ion AmpliSeq™ technology delivers simple and fast library construction for affordable targeted sequencing of specific genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq technology requires minimal input DNA or RNA to target sets of genes, enabling sequencing of FFPE samples. Choose from among a wide variety of ready-to-use, custom, and community Ion AmpliSeq™ panels to transform your cancer research using FFPE tissue samples.

  • Single-day workflow from DNA to results
  • Multiple applications—predesigned and custom panels targeting up to thousands of genes for germline and somatic variant detection, gene expression analysis and gene fusion identification

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Ion Chef System

Minimize hands-on time with the Ion Chef System for template prep

The Ion Chef™ System simplifies the sequencing workflow and provides flexibility for any project type and size by supporting all Ion semiconductor chips and template preparation methods for a broad range of DNA and RNA libraries. With minimal hands-on time, walk-away automation, and up to two sequencing-ready chips per run, the Ion Chef System not only helps save time and labor; but also enables overnight runs to help maximize your lab's productivity.

  • Simple—enables walk-away automation of template preparation and chip loading in a single system for the Ion PGM, Ion Proton™, Ion S5, and Ion S5 XL systems
  • Reproducible—automated workflow and on-board sample and reagent tracking help reduce user-introduced variability
  • Efficient—helps save time and labor; setup in just15 minutes, and load up to two Ion semiconductor sequencing chips per run

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Ion PGM System

Enables a simple and fast workflow for targeted sequencing

The Ion PGM™ System, coupled with Ion AmpliSeq technology and Ion Reporter™ Software, provides a complete end-to-end solution, from base calls to variants, and from low-input and degraded sample types such as FFPE tissue.

  • Powerful next-generation sequencing technology to get answers faster, as part of a single-day workflow from input DNA to variant calls
  • Offers a range of fast and simple library solutions and kits with low sample input requirements for multiple research applications such as gene panel sequencing and RNA sequencing

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Ion Reporter Software

Accelerate time-to-results with Ion Reporter Software

Accelerate your time-to-results with optimized and automatable workflows for Ion AmpliSeq™ Targeted Gene Panels, and go from sample to meaningful variants in a single day. Simple results filtering with annotations from >20 public databases makes it easy to focus on the variants important to you. Ion Reporter Software provides an optimized suite of simple data analysis tools that streamline Ion PGM and Ion Proton system data analysis, so you can focus on finding the biological meaning of your data.

  • Simple—straightforward data analysis with easy-to-use preconfigured and customizable workflows provides flexibility for users of any experience level
  • Streamlined—quickly focus on your variants of interest, then save your filter settings for standardized workflows
  • Secure—analyze and securely share data in the cloud, or analyze and store data on-site

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Sanger Sequencing, Real-Time PCR, and Digital PCRTop

Applied Biosystems Minor Variant Finder Software

5% somatic variant detection using Sanger sequencing

Minor Variant Finder is developed for the detection and reporting of minor mutations by Sanger sequencing. Minor variants are single nucleotide polymorphisms (SNPs), which present as a minor component with a contribution of less than 25% at a given allele. The ability to detect low-level somatic mutations is critical in research areas such as oncology, infectious disease, and inherited disease.

  • Call minor variants at detection level as low as 5%
  • Sequence a moderate number of targets at a low cost
  • Provide an easy and flexible way to augment fixed gene panels
  • Confirm NGS findings in alignment view and Venn diagram

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Applied Biosystems 3500 Series Genetic Analyzers

Innovative. Intelligent. Intuitive.

The 3500 Series Genetic aAnalyzers are specifically designed to support the demanding performance needs of validated and regulated environments while retaining the unsurpassed application versatility that life science researchers expect from Applied Biosystems™ instruments. Designed with you in mind, the 3500 Series Genetic Analyzers offer:

  • Automated operation
  • Easy-to-install consumables
  • Electronic tracking of instrument performance and maintenance tasks
  • Integrated analysis software

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Applied Biosystems QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems

Real-time PCR systems designed around easy access and data sharing

The QuantStudio™ 3 and QuantStudio™ 5 Real-Time PCR systems are the first real-time PCR systems enabling collaboration anywhere, anytime. Easily store, retrieve, and share data using the QuantStudio 3 and 5 systems integrated with a powerful cloud platform: Thermo Fisher Cloud. With an interactive instrument touchscreen for when you're in the lab, and remote monitoring when you're not, QuantStudio 3 and 5 take real-time PCR to places you never thought possible.

  • Flexible instrument control and data management—choose a completely standalone instrument or co-locate with a PC or Mac laptop computer and Thermo Fisher Cloud
  • Access experiment runs from any location, anytime—has WiFi connectivity and is enabled for remote monitoring using Thermo Fisher Cloud
  • Easy and fast on-the-fly analysis—use portable devices like tablets to quickly analyze data when you need it through Thermo Fisher Cloud
  • Analyze sophisticated data sets in minutes—uses Applied Biosystems™ qPCR analysis modules for advanced analysis functionality and integrated analysis of multiple data sets online
  • Securely share data sets and protocols online—collaborate with colleagues across the campus and around the world in a secure cloud environment

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Applied Biosystems QuantStudio 3D Digital PCR System

Quantify rare mutations at a prevalence as low as 0.1%

The QuantStudio™ 3D rare mutation analysis solution includes a subset of wet lab - (lab-validated) validated TaqMan™ SNP Genotyping Assays to detect and quantify the most common cancer-related mutations. These assays were designed using the full bioinformatics power of the TaqMan Assay design pipeline and were validated specifically for the QuantStudio™ 3D Digital PCR System. Together, they offer researchers the ability to achieve the high performance required to detect and quantify mutations present at very low frequencies.

  • Optimized digital PCR performance—wet lab - (lab-validated) validated TaqMan Assays targeting 60 somatic mutations for the QuantStudio 3D Digital PCR System
  • High sensitivity—detect and quantify rare mutant prevalence as low as 0.1%
  • Cost-effective and convenient—single-tube format includes both wild type and mutant alleles small and large reaction options
  • Streamlined analysis—advanced algorithms for better quantification of rare mutations
  • Guaranteed performance—wet lab - (lab-validated) validated assay replaced or account credited if the assay does not perform to the standards of the guarantee*

*Terms and conditions apply. For complete details, click here. 

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Flow Cytometry, Protein Analysis, and AntibodiesTop

Invitrogen Attune NxT Acoustic Focusing Cytometer

Pioneer new breakthroughs in rare-event detection

To give cancer researchers the precision and sensitivity needed to better identify rare circulating tumor cells or detect subtle aberrant changes in cell cycle.

  • Modular design
  • Fast detection speed
  • Distinctive acquisition and analysis software
  • Convenient size

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Invitrogen Mini Gels and Thermo Scientific Pierce Power Stainer

Save time with a comprehensive western workflow for protein separation, transfer, and detection

The combination of Bolt™ gels and the Thermo Scientific™ Pierce™ Power Stainer enables you to load twice the amount of sample and obtain protein separation results in as little as 10 minutes, versus 4–12 hours using manual methods. Additionally, iBind™ and Thermo Scientific™ SuperSignal™ substrates help you save precious primary antibodies and hands-on processing time, all with better sensitivity than manual methods.

  • Wedge well helps to enable superior resolution
  • Longer shelf life with the convenience of ambient storage
  • Mini Gel Tank versatility—one tank, now compatible with 181 of your favorite NuPAGE™, Bolt, and Novex™ mini gels
  • Pierce Power Stainer—high-quality protein staining with low background in about 10 minutes
  • Thermo Scientific™ Pierce™ Power Blotter—high-quality transfer of 100–300 kDa proteins in about 10 minutes
  • SuperSignal detection substrates—excellent performance with longer light emission and stronger signal intensity than other luminol-based detection systems
  • iBind system—automated device for blot processing

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Invitrogen™ Antibodies, ELISAs, and Luminex™ Assays

Singleplex, multiplex, and custom assays

  • Over 60,000 primary, secondary and custom antibodies
  • Over 1,000 ELISA and multiplex Luminex assay kits

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Cell Culture, Imaging, and AnalysisTop

Thermo Scientific CellInsight CX5 High Content Screening Platform

Compact, affordable screening to fit your lab

Fast scanning and quantitative analysis for high-content screening

  • Quantitative fluorescence and bright field imaging
  • Full automation of plate handling, scanning and analysis
  • Compact footprint for lab bench or robotic line
  • HCS Studio™ cell analysis software provides comprehensive analysis tools

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Invitrogen EVOS FL Auto Imaging System

Powerful. Brilliant. Flexible. Intuitive.

The EVOS™ FL Auto Imaging System and EVOS™ Onstage Incubator operate as one fully integrated unit, for an exceptional live-cell imaging experience. They enable live-cell imaging, area scanning, image stitching, and time-lapse imaging with a simple touch of the monitor.

  • Fully integrated environmental chamber for live-cell time-lapse imaging
  • Precise — maintain physiological or hypoxic conditions
  • Intuitive — set all acquisition parameters from the EVOS™ FL Auto interface
  • Small footprint, robust build and workflow-enhancing design elements
  • Choice from a range of vessel holders

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Thermo Scientific Nunclon Sphera Products

Grow cancer spheroid cultures with ease and consistency

Nunclon™ Sphera™ products outperform non-treated surfaces for cancer spheroid cultures while consistently and repeatedly generating favorable numbers of cancer spheroids for studying tumor cell progression and efficacy of anticancer agents in vitro.

  • Support consistent formation of cancer spheroids
  • Ultralow-binding surface available in flasks, dishes, and 96-well plates

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Thermo Scientific Nunc Edge 96-Well Plate

Take your cell-based assays to the edge

The one-of-a-kind design of Nunc™ Edge™ Plates edge effect and enables use of all 96 wells during long-term incubation. Enabling improved consistency in assay models for in vitro research, the unique design of the Edge Plate helps increase output per plate by more than 37% while maintaining cell health and viability in all wells.

  • Evaporation moat prohibits edge effect
  • Gain full capacity of your plate with consistent cell viability in all 96 wells 

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Invitrogen™ GeneArt™ Engineered CRISPR

Cell models for cancer research

  • Matched isogenic cell line pairs as disease models 
  • More than 350 different genotypes, with a focus on oncology models
  • Over 30 different cell line backgrounds (e.g., HCT116, DLD-1, SW48, RKO, MCF10A)
  • In-depth validation—cellular identity STR/SNP profiling and genomic characterization 

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HPLC Mass SpectrometryTop

Thermo Scientific Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer

Ideal for protein identification, intact protein analysis, shotgun lipidomics, metabolomics, and high-throughput proteomic and screening workflows, targeted quantification and unknown retrospective identification

  • Identify proteins in complex biological matrices; typically without digestion
  • Detect degradation products, sequence variants and combinations of post-translational modifications
  • Quantify in a high throughput workflow 

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Thermo Scientific TSQ Quantiva Triple Quadrupole Mass Spectrometer

Selected-reaction monitoring (SRM) Gold standard for targeted protein quantification

The TSQ Quantiva™ Triple Quadrupole Mass Spectrometer exceeds the most stringent analytical requirements for quantitative performance with attogram-level sensitivity, unprecedented usability and exceptional robustness.

  • Quantify well characterized compounds in a high throughput workflow.
  • Obtain the sensitivity and speed ideally suited for complex biological matrices
  • Determine the protein and/or peptide expression levels of known targets in biological systems

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Thermo Scientific Vanquish UHPLC Systems

More results with better separations, without compromise

These easy-to-use, fully integrated and biocompatible systems feature high sample capacity for high-throughput workflows, industry-leading pumping performance, exceptionally high S/N and linearity, two-mode thermostatting, and active preheating.

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Sample PreparationTop

Applied Biosystems ArcturusXT Laser Capture Microdissection (LCM) System

Isolate pure cell populations from tumor tissues

Laser capture microdissection (LCM) is a powerful tool for separating and capturing selected populations of tumor cells from heterogeneous tumor samples, enabling you to consistently obtain clear and accurate results from downstream analysis.

  • Dual laser system—Infrared (IR) and ultraviolet (UV) lasers enable a wide range of applications from single-cell isolation to large biopsy extractions
  • Intuitive software—enables rapid isolation of specific cells in five simple steps and allows for visualization of the collected sample for quality control
  • Flexible sample prep—wide range of slide types and sample preparation formats supported to optimize workflows

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Applied Biosystems MagMAX Cell-Free DNA Isolation Kits

Optimize gDNA isolation from biological fluids and cell-free samples

The demands for DNA yield and purity have become more stringent with the development of new analytical methods and technologies, such as
high-throughput genotyping, and next-generation DNA sequencing platforms. MagMAX™ DNA Multi-Sample kits provide a consistent, reliable method for the rapid purification of high-quality genomic DNA.

  • Designed for downstream applications—the MagMAX kits help you obtain cleaner, more abundant nucleic acid that enable efficient downstream applications
  • Flexible, scalable format—by utilizing magnetic beads, these kits provide a flexible format that is easily scalable and enables better binding and washing efficiencies than filter-based technologies

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Sample Purification, Quantitation, and Gene ExpressionTop

ThermoScientific NanoDrop One Microvolume UV-Vis Spectrophotometers

Quantify and qualify DNA, RNA or protein samples in seconds

Built as standalone spectrophotometers with Thermo Scientific™ Acclaro™ Sample Intelligence technology, these next-generation NanoDrop™ instruments can:

  • Accurately measure 1-2 µL of sample without dilutions
  • Identify common contaminants and calculate corrected concentrations
  • Provide instant feedback and guided troubleshooting
  • Auto-Measure feature streamlines workflows and organizes data.
  • Export data to a PC via USB, Ethernet or Wi-Fi for electronic archiving or printing.

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Thermo Scientific KingFisher Duo Prime Magnetic Particle Processor

Isolation applications that process virtually any sample from any source

The Thermo Scientific™ KingFisher™ Duo delivers advanced functionality in a compact, mid-throughput–capacity instrument for isolation applications to help get results more efficiently enabling you to reap high yields of quality nucleic acids from a variety of samples.

  • Revolutionary proprietary separation technology lets you process virtually any sample from any source for the ultimate in isolation of nucleic acids, proteins, and cells
  • Process up to 24 samples on one protocol at a time
  • New UV decontamination, barcode reader, and elution strip cover provide an enhanced user experience

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Invitrogen Qubit 3.0 Fluorometer

Designed for your precious samples and high-value applications

Qubit™ fluorometric quantitation enables accurate data through the use of target-specific fluorescent dyes and assay reagents that perform even in the presence of common contaminants and degraded samples. 

  • Large, 5.7" touchscreen for easy workflow navigation and effective data management, with the ability to store up to 1,000 sample results
  • Rapid results that involve as little as 2 minutes assay incubation and ~5 seconds sample read time

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Invitrogen™ siRNA Tools

Still an easy and quick way to knock down a gene

  • Specific libraries for oncology research
  • Genome-wide, predesigned, and custom solutions
  • Effective delivery and superior knockdown with fewer off-target effects
  • Proprietary chemical modifications to maximize potency and specificity with guaranteed knockdown*

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* For details on the siRNA guarantee, visit the siRNA Guarantee page here.

Invitrogen™ Novex™ WedgeWell Tris-Glycine Gels

Discover a whole new depth in Tris-glycine gels

  • Wedge-shaped wells for loading up to 60 μL of sample
  • Excellent protein band resolution and sharpness
  • Store for up to 12 months at 4°C
  • Compatible with native and denatured protein samples

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