ExpiSf Expression System

The only optimized insect expression system with superior protein yields: Gibco ExpiSf Expression System

Experience the first-ever chemically defined baculovirus expression system that can generate three times more protein compared to existing insect expression platforms. The Expisf Expression System enables greater consistency across multiple expression runs, all in less time. Put the newest member of the Expi system family to the test in your lab, from discovery to production.

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Discover the Gibco ExpiSf Expression System

“The new ExpiSf Expression System offers a convenient virus generation and protein expression cell line in only two steps, for both secreted and intracellular proteins,we predict that using the ExpiSf system will save us about 30 percent in the time taken for protein expression and make cell maintenance simpler.”  Xuan Droz, Ph.D., project leader, Redbiotec AG

The first-ever chemically defined insect expression system

Undefined insect media components like yeastolate make it very difficult to get consistent performance. The Gibco ExpiSf system is the first ever baculovirus expression system that includes both a chemically defined medium and protein expression enhancer for reliable results you can count on.

Gibco ExpiSf Expression System consists of multiple components

Included in the system:

  1. Two vials of frozen ExpiSf9 Cells
  2. 1 L ExpiSf CD Medium
  3. One Bac-to-Bac Vector Kit*
  4. 4 mL ExpiSf Enhancer
  5. 0.5 mL MAX Efficiency DH10Bac Competent Cells*
  6. 1 mL ExpiFectamine Sf Transfection Reagent
  7. 500 mL Opti-MEM I Medium, with phenol red*
  8. 24 Nalgene Single-Use PETG vented 125-mL Erlenmeyer Flasks**

**not included in China
** not included in all regions

ONE system with remarkably consistent results

The ExpiSf Expression System is designed to deliver consistent cell growth and protein expression, run after run. With our new Sf9 expression system, inconsistent results are a thing of the past. The superior performance of the chemically defined ExpiSf system delivers consistent results across expression runs and over multiple cell passages. One reliable system gives you one less variable to worry about.

ONE system for superior protein yields

It’s revolutionary—the ExpiSf system outperforms existing Sf9 expression platforms with 3x higher protein yield. The ExpiSf Expression System achieves protein yields that are vastly superior to traditional insect platforms. In performance tests against other suppliers’ media, the ExpiSf system demonstrated greater protein expression, delivering 3x more protein.

Figure 1. Recombinant protein titers in ExpiSf and other baculovirus expression systems. Expression levels of green fluorescent protein (GFP), Fc fusion protein, and tumor necrosis factor alpha (TNF-a) in ExpiSf and other baculovirus-based insect protein expression products are shown. ExpiSf protein titers are >3, >4, or >5 times those of other supplier’s insect products, respectively.

ExpiSf System cost effectiveness

Table 1. ExpiSf is cost effective compared to other products for baculovirus-based insect protein expression systems.

NOTE: Average cost to express 100 mg of protein with ExpiSf System or a combination of competitor products. Cost show is based on list price in USD.
  Thermo Fisher Thermo Fisher Thermo Fisher Expression Systems MilliporeSigma Oxford Expression Technologies Hyclone
Cells ExpiSf9 Sf9 Sf9 Sf9 Sf9 Sf9 Sf9
Medium ExpiSf CD Sf-900 II SFM Sf-900 III SFM ESF 921TM ExCellTM 420 ESF 921TM SFX-InsectTM
Transfection Reagent ExpiFectamine Sf Cellfectin II Cellfectin II
Expres2 TR Insect GeneJuiceTM Expres2 TR Cellfectin II
Baculovirus System Bac-to-Bac Bac-to-Bac Bac-to-Bac BestBacTM BacMagicTM flashBACTM Bac-to-Bac
Cost of 1L expression $334 $235 $260 $186 $332 $110 $243
Culture volume (mL) for equivalent yield* 300 1200 1700 3100 1700 3100 1500
Number of 2L shake flasks needed 1 2 3 6 3 6 3
Total cost for same amounf of protein $112 $294 $454 $579 $563 $343 $368
Savings   $65 $226 $351 $335 $115 $139


ONE optimized and fully integrated system

System components work synergistically for maximum performance. Other suppliers offer separate reagents for protein production using the baculovirus expression vector system, but there is only one system that’s fully optimized with a streamlined workflow. The ExpiSf system contains integrated components that work together to deliver maximum protein yields.

The ExpiSf system contains integrated components that work together to deliver maximum protein yields

ONE step from baculovirus production to protein expression

The ExpiSf Expression System delivers protein in half the time. Bacmid DNA transfection in suspension using the ExpiSf system allows for efficient baculovirus generation without the need for virus amplification. That means you can get your protein in as few as 6 days.

Standard protocol for baculovirus generation compared to shorter protocol with ExpiSf Expression System for baculovirus generation.

ONE system that scales from discovery to production

ExpiSf CD Media

The ExpiSf system accelerates development from small to large scale. From deep-well plates to shake flasks, the ExpiSf system delivers the same volumetric yield, making the system excellent for both discovering new therapies and producing them in large quantities. We manufacture ExpiSf CD Medium in cGMP-compliant facilities and offer custom packaging to scale with your development, so you can be confident that our product quality enhances yours.

The ExpiSf expression system delivers the same volumetric yield

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