Dynabeads® Protein G for Immunoprecipitation

Catalog number: 10004D

Novex™  Related applications: Antibody Purification | Chromatin Biology | Protein Assays and Analysis | Protein Purification & Isolation

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Description

Dynabeads® Protein G are uniform, 2.8 µm superparamagnetic beads with recombinant Protein G (approximately 17 kDa) covalently coupled to the surface. Dynabeads® Protein G provide a superior alternative to Sepharose® or agarose slurry for immunoprecipitation (IP):

• Simple, short ~30 minute procedure
• Significantly reduces background caused by nonspecific binding
• No columns, centrifugations, or time-consuming pre-treatment of your samples
• Gentle separation technology preserves native protein conformations and large-protein complexes

Magnetic bead-based separation is rapid and easy to perform
Antibody is added to the Dynabeads® Protein G. During a short incubation, the antibody binds to the Dynabeads® via their Fc-region. The tube is then placed on a magnet, where the beads migrate to the side of the tube facing the magnet. The supernatant is then removed by aspiration. The bead-bound antibody can now be used for a variety of downstream applications including immunoprecipitation, co-IP, small-scale IgG purification and antibody labeling, chromatin immunoprecipitation (ChIP), RNA IP (RIP), and protein isolation. Bound material is easily collected on a DynaMag™ magnet, utilizing the unique magnetic properties of the Dynabeads®. The recombinant protein G on the beads contains no albumin binding sites, thus albumin is not co-purified during the procedure.

Gentle separation causes minimal physical stress to proteins
The magnetic separation technology utilized by Dynabeads® Protein G is rapid and gentle, causing minimal physical stress to your target proteins. This permits the isolation and concentration of labile composites that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and large protein complexes are preserved.

Binding strength and capacity
Dynabeads® Protein G allow for isolation of most mammalian immunoglobulins (Ig). (The Binding Strengths table below gives the binding strengths for different species and subclasses.) The amount of Ig captured depends on the concentration of Ig in the starting sample and on the type and source of the Ig. 100 µL Dynabeads® Protein G will isolate approximately 25–30 µg human IgG from a sample containing 20–200 µg IgG/mL. Predominant Fc binding allows optimal Ig orientation

Useful for many sample types
Using Dynabeads® Protein G, antibodies can be purified from saliva, plasma, ascites, serum and tissue culture or hybridoma supernatants.

Learn more about Dynabeads®
• Dynabeads® Protein G are also available as a "ready-to-go" kit with buffers included.
• Find selection guides, data, and references that support the utility of Dynabeads® for immunoprecipitation.
• Find magnets for Dynabeads® separations.
• Find Dynabeads® products for other applications.

OEM purchase
To purchase Dynabeads® Protein A and Protein G on an OEM basis, contact our Out-Licensing and OEM Sales department.

*Sepharose® is a trademark of GE Healthcare companies.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Ligand Type: Protein G
Form: Beads in Suspension
Shelf Life: 24 months from date of manufacture
Product Size: 5 mL
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Room Temperature

Contents & storage

Contains: 5 mL Dynabeads® Protein G
Storage: 2°C to 8°C.

Documents

Manuals & protocols

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