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MCAM (CD146) Recombinant Human Protein, hIgG1-Fc Tag

Catalog number: 10115H02H250

 Related applications: Protein Assays and Analysis

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Description

Melanoma cell adhesion molecule (MCAM/CD146), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gly 559) of human MCAM precursor (NP_006491.2) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line . When 3 x 104 cells/well are added to human CD146 coated plates (0.8 µg/ml, 100 µl/well), approximately >30 % will adhere after one hour at 37°C.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Gene ID: 4162
Species: Human
Expression: Mammalian
Gene Symbol: MCAM
Gene Aliases: MCAM, CD146, MUC18
Product Size: 5 x 50 µg
Protein Form: Truncated, Recombinant
Protein Family: CD Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: Other Proteins
Molecular Weight: 86.4 kDa
Research Category: Immunology, Angiogenesis, Development, Extracellular Matrix & Cell Adhesion, Cardiovascular Research, Oncology, Signal Transduction
Protein Tag or Fusion: IgG1 Fc Tag
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols