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IL1R1 Recombinant Human Protein, hIgG1-Fc Tag

Catalog number: 10126H02H50

 Related applications: Protein Assays and Analysis

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Description

Interleukin 1 receptor, type I (IL1R1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in cell biology research applications. It is suitable for use as protein standard or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 332) of human IL1R1 (NP_000868.1) fused to the human IgG1 Fc region at the C-terminus.

Activity:
Measured by its binding ability in a functional ELISA:
1. Immobilized human IL1B at 20 µg/ml(100 µl/well) can bind human IL1R1-Fc with a linear range of 6.4-160 ng/ml.
2. Immobilized human IL1B-His at 10 µg/ml(100 µl/well) can bind human IL1R1-Fc. The ED50 of human IL1R1-Fc is 0.198 µg/ml.
3. Immobilized human IL1B at 10 µg/ml(100 µl/well) can bind human IL1R1-Fc. The ED50 of human IL1R1-Fc is 0.48 µg/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Lyophilized
Purity: ≥ 85% by SDS-PAGE
Gene ID: 3554
Species: Human
Expression: Mammalian
Gene Symbol: IL1R1
Gene Aliases: CD121A, D2S1473, IL-1R-alpha, IL1R, IL1RA, P80, IL1R1
Product Size: 50 µg
Protein Form: Truncated, Recombinant
Protein Family: Cytokines & Receptors, CD Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: Interleukins, Cytokine Receptors
Molecular Weight: 63 kDa
Research Category: Immunology, Inflammation, Signal Transduction
Protein Tag or Fusion: IgG1 Fc Tag
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols

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