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PECAM1 Recombinant Human Protein, hIgG1-Fc Tag

Catalog number: 10148H02H50

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Description

Platelet/Endothelial Cell Adhesion Molecule 1 (PECAM1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 601) of human PECAM1 (EAW94208.1) fused to the fused Fc region of human IgG1 at the C-terminus.

Activity: Measured by its ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells.
When cells are added to recombinant human PECAM1 coated plates (3.3 µg/ml, 100 µl/well) in the presence of PMA, approximately 50%-65% will adhere specifically after 30 minutes at 37°C.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Species: Human
Expression: Mammalian
Gene Symbol: PECAM1
Gene Aliases: PECAM1, CD31, PECAM-1
Product Size: 50 µg
Protein Family: CD Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: Intercellular adhesion Molecule
Molecular Weight: 91.2 kDa
Research Category: Cardiovascular Research, Inflammation, Microbiology
Protein Tag or Fusion: IgG1 Fc Tag
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols