Express Five® SFM

Catalog number: 10486025

Gibco™  Related applications: Insect Cell Culture

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Description

Express Five® SFM is a protein-free, serum-free medium specifically developed to support the long-term suspension growth of High Five™ (BTI-TN-5B1-4) cells in suspension for use in the Baculovirus Expression Vector System (BEVS). This medium requires the addition of 16 mM L-glutamine before use. Gibco® Express Five® SFM features:

• Superior long-term suspension growth of High Five™ cells
• Protein-free, serum-free formulation
• Optimized for recombinant protein production
• Scalable in the CelliGen® bioreactor and WAVE Bioreactor® System

Superior growth of High Five™ cells
Gibco® Express Five® SFM provides peak cell densities of up to 6 × 106 viable cells per mL in shaker cultures. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® Express Five® SFM, usually without any further adaptation (see figure). Cells usually require adaptation from serum-containing formulations.

Protein-free, serum-free formulation
Gibco® Express Five® SFM is a protein-free and serum-free formulation, allowing for easier purification of your protein of interest.

Optimized for recombinant protein expression
Gibco® Express Five® SFM is optimized for high protein production using the Baculovirus Expression Vector System (BEVS). Using BEVS, we routinely achieve rβ-galactosidase protein expression of up to 1.7 U/cell, a significant improvement over other media (see figure)

Scalable in the CelliGen® bioreactor and WAVE Bioreactor® System
Protein production using Gibco® Express Five® SFM can be scaled up in bioreactors such as the 5 L CelliGen® bioreactor and the WAVE Bioreactor® System. The appropriate impeller or rocking speed and seeding density should be optimized for each system. Depending on the bioreactor design, it may be necessary to supplement Gibco® Express Five® SFM with additional Pluronic® F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco® Express Five® SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Cell Line: High Five™
Cell Type: Insect Cells
Glutamine: No Glutamine
Serum Level: Serum-Free
Product Size: 1,000 mL
Classification: Serum-Free, Protein-Free
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture

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Manuals & protocols

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