E-Gel® 1 Kb Plus DNA Ladder

Catalog number: 10488090

Invitrogen™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting

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Description

The E-Gel® 1 Kb Plus DNA ladder is a powerful tool for estimating the molecular weight of linear, double-stranded DNA fragments. The E-Gel® 1 Kb Plus DNA ladder is composed of 20 highly purified, double-stranded DNA bands spanning 100 base pairs to 12,000 base pairs. The ladder has 12 evenly spaced bands ranging from 1 kb to 12 kb, a quick orientation band at 1,650 base pairs that forms a distinct doublet with the 2 kb band, and seven bands of round sizes below 1 kb. The E-Gel® 1 Kb Plus DNA Ladder is specifically formulated with minimal salts to perform exceptionally well on E-Gel® EX pre-cast agarose gels. Features of the E-Gel® 1 Kb Plus DNA Ladder:

• Round, easy-to-remember band sizes
• Broad size range (100 bp–12 kb)
• Formulated specifically for E-Gel® EX gels
• Room temperature stable for easy storageOur most popular and easiest-to-use ladder
The E-Gel® 1 Kb Plus DNA ladder is our most popular E-Gel® DNA ladder used to estimate molecular mass of double-stranded DNA bands from PCR, restriction digests, and other molecular biology protocols. Unlike some other DNA ladders, the E-Gel® 1 Kb Plus DNA Ladder has been specially designed to have bands of round, easy-to-remember sizes. The logical band layout, and quick identification doublet, allow you to quickly and easily estimate the size of your band of interest at a glance. The migration pattern of E-Gel® 1 Kb Plus DNA Ladder is shown (see figures).

A single ladder for most of your needs
The 20 bands comprising E-Gel® 1 Kb Plus DNA Ladder range in size from 100 bp up to 12 kb, making it one of the broadest DNA ladders available. This wide breadth is perfect for downstream cloning applications as well as end-point PCR and other molecular biology applications.

Ready-to-use formulation for E-Gel® and E-Gel® EX gels
The E-Gel® 1 Kb Plus DNA Ladder is compatible with all E-Gel® products, and is specifically formulated to be compatible with salt-sensitive E-Gel® EX gels. The ladder is provided premixed with loading and tracking buffers that also provide room temperature stability for easy storage. You’ll receive 500 µL of the E-Gel® 1 Kb Plus DNA Ladder, which is enough for 100 loads.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Compatibility: E-Gel®
Ready to Load: Yes
Mass: 17.5 µg
Includes: Tartrazine, Xylene Cyanol
Size Range: 0.1 to 12 kb
Product Size: 100 applications
Concentration: 35.0 ng⁄µl
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & storage

E-Gel® 1 Kb Plus DNA Ladder is supplied at 0.035 µg⁄µl in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA (pH 8.0), 0.0075% XCFF, 0.05% tartrazine, and 5% glycerol. Store E-Gel® 1 Kb Plus DNA Ladder at room temperature. This ladder is guaranteed stable for 6 months when properly stored.

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