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TNF (TNFa) Recombinant Human Protein

Catalog number: 10602HNAE5

 Related applications: Protein Assays and Analysis

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Description

Manufactured by Sino Biological (China); Distributed by Life Technologies.

Tumor necrosis factor alpha (TNF-α) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein is expressed from a DNA sequence encoding the soluble form of human TNF (NP_000585.2) (Val 77-Leu 233) retaining the initial Met at the N-terminus.

N-terminal Sequence Analysis: Met.

Activity: measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is typically 25-200 pg/mL, corresponding to a specific activity of 5 x 106 to 4 x 107 units/ mg.

Formulation: lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Background
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-α is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is primarily produced as a type I I transmembrane protein arranged in stable homotrimers, and a soluble trimeric form of TNF-α is released via proteolytic cleavage by the metalloprotease TACE/ ADAM17. Two receptors mediating the TNF-α function have been identified, the ubiquitous TNF RI from which most signaling are derived, and the hematopoietic cell-restricted TNF RII. Upon the TNF-α trimers binding, TNF receptors also form trimers, undergo a conformational change, and enable the adaptor protein TRADD to bind to the death domain, and thus initiates the signal pathways such as the NF-kB, Jak/STAT, and the MAPK pathways, as well as the death signaling. As a major mediator of apoptosis, inflammation and immunity, TNF-α has been implicated in the pathogenesis of a variety of human diseases including autoimmune diseases, insulin resistance, and cancer.

You may also be interested in the following TNF-α products:
ELISA Kits
Primary Antibodies
Pathway Analysis Kits
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Lyophilized
Purity: ≥ 95% by SDS-PAGE
Species: Human
Expression: E. coli
Gene Symbol: TNF
Gene Aliases: TNF, DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2
Product Size: 5 µg
Protein Form: Full Length, Recombinant
Protein Family: Cytokines & Receptors
Protein Subtype: TNF (Tumor Necrosis Factor)
Molecular Weight: 17.4 kDa
Research Category: Immunology, Inflammation, Signal Transduction, Oncology
Protein Tag or Fusion: Untagged
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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