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MET (c-MET) Recombinant Human Protein (without Catalytic Activity Domain), His Tag

Catalog number: 10692H08H50

 Related applications: Protein Assays and Analysis

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Description

Met proto-oncogene/hepatocyte growth factor receptor (MET/c-MET), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies..

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human MET (NP_000236) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 10 µg/ml (100 µl/well) can bind biotinylated MET. The ED50 of biotinylated Met is 5.28 µg/ml.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Lyophilized
Purity: ≥ 85% by SDS-PAGE
Gene ID: 4233
Species: Human
Expression: Mammalian
Gene Symbol: MET
Gene Aliases: MET, AUTS9, HGFR, RCCP2, c-Met
Product Size: 50 µg
Protein Form: Truncated, Recombinant
Kinase Family: MET (HGFR) Kinase Family
Protein Family: Growth Factors & Receptors
Endotoxin Level: < 1 EU/µg
Protein Subtype: Growth Factor Receptors, Receptor Tyrosine Kinases
Molecular Weight: 103.0 kDa
Research Category: Signal Transduction, Oncology, Stem Cell Research
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols