10 bp DNA Ladder This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 10821015

Invitrogen™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting

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Description

The 10 bp DNA Ladder is suitable for sizing double-stranded DNA or single-stranded DNA from 10 bp to 330 bp. It consists of 33 ten base pair repeats.

Product use
The double-stranded DNA ladder can be visualized on 4% to 5% agarose gels (for 10 bp to 150 bp fragments), 2% to 3% agarose gels (for 150 bp to 330 bp fragments), 2% to 3% Agarose 1000 gels, or 6% to 10% polyacrylamide gels after ethidium bromide staining. Because both DNA strands are of the same nucleotide composition, this product can be denatured to produce a set of single-stranded oligonucleotides increasing in length by 10-nucleotide increments. The single-stranded ladder can be visualized on a 6% urea-polyacrylamide gel after electrophoresis. For easy reference on agarose gels, the 100 bp band is two to three times brighter than the other bands in the ladder. This ladder can be stained with ethidium bromide or SYBR® Green dyes, or radiolabeled using T4 polynucleotide kinase.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Compatibility: Agarose Gels
Ready to Load: No
Mass: 50 µg
Size Range: 10 to 330 bp
Product Size: 50 µg
Concentration: 1 µg⁄µl
Volume (Metric): 50 µl
Green Features: Less waste, Sustainable packaging
Shipping Condition: Approved for shipment at Room Temperature or on Dry Ice

Contents & storage

10 bp DNA Ladder is supplied at 1 µg/µl in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA. Store at -20°C.

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